HSP27 antibody

Catalog No. ABIN966314

There is 1 publication for this product available. The Rabbit Polyclonal anti-HSP27 antibody is suitable to detect HSP27 in samples from Human. It has been validated for IHC.

Quick Overview for HSP27 antibody (ABIN966314)

Target: HSP27 (HSPB1) (Heat Shock 27kDa Protein 1 (HSPB1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HSP27 antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Product Details anti-HSP27 Antibody

Specificity: HSP27 (Ab-82) antibody detects endogenous levels of total HSP27 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human HSP27 around the phosphorylation site of serine 82 (Q-L-SP-S-G).

Application Details

Application Notes: WB: 1:500~1:1000
IHC: 1:50~1:100.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100ug/100ul.

Buffer: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 50% glycerol

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

References for anti-HSP27 antibody (ABIN966314)

White, Hambly, Jeremy, Cordwell: "Ischemia-specific phosphorylation and myofilament translocation of heat shock protein 27 precedes alpha B-crystallin and occurs independently of reactive oxygen species in rabbit myocardium." in: Journal of molecular and cellular cardiology, Vol. 40, Issue 6, pp. 761-74, (2006) (PubMed).

Target Details for HSP27

Target: HSP27 (HSPB1) (Heat Shock 27kDa Protein 1 (HSPB1))

Alternative Name: HSP27

Pathways: MAPK Signaling, Regulation of Actin Filament Polymerization, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, VEGF Signaling