LCK antibody

Catalog No. ABIN966480

Cited in 3+ publications. The Monoclonal anti-LCK antibody (ABIN966480) specifically detects LCK in IHC. The antibody is reactive with Human samples.

Quick Overview for LCK antibody (ABIN966480)

Target: LCK (Lymphocyte-Specific Protein tyrosine Kinase (LCK))

Reactivity: Human

Host: Please inquire

Clonality: Monoclonal

Conjugate: This LCK antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Product Details anti-LCK Antibody

Specificity: Ni-NTA purified truncated recombinant Lck expressed in E. Coli strain BL21 (DE3)

Purification: Crude ascites.

Isotype: IgG2a

Application Details

Application Notes: Western Blot: 1: 500- 1: 2,000
ELISA: Propose dilution 1: 10,000.
Determining optimal working dilutions by titration test.

Restrictions: For Research Use only

Handling

Format: Liquid

Storage: -20 °C

References for anti-LCK antibody (ABIN966480)

Mingjian, Wheeler, Cheng, Yonghai, Chen, Fitzwarryne, Wang: "HIV/AIDS prevalence and behaviour in drug users and pregnant women in Kashgar Prefecture: case report." in: Harm reduction journal, Vol. 3, pp. 7, (2006) (PubMed).

Stachlewitz, Hart, Bettencourt, Kebede, Schwartz, Ratnofsky, Calderwood, Waegell, Hirst: "A-770041, a novel and selective small-molecule inhibitor of Lck, prevents heart allograft rejection." in: The Journal of pharmacology and experimental therapeutics, Vol. 315, Issue 1, pp. 36-41, (2005) (PubMed).

Hawash, Kesavan, Magee, Geahlen, Harrison: "The Lck SH3 domain negatively regulates localization to lipid rafts through an interaction with c-Cbl." in: The Journal of biological chemistry, Vol. 277, Issue 7, pp. 5683-91, (2002) (PubMed).

Target Details for LCK

Target: LCK (Lymphocyte-Specific Protein tyrosine Kinase (LCK))

Alternative Name: Lck

Background: Lck(lymphocyte-specific protein tyrosine kinase), with 509-amino acid protein (about 56kDa), belongs to the Src non-receptor tyrosine kinases family. By virtue of common structural motifs, the Src family is composed of nine members in vertebrates, including Src, Yes, Fgr, Frk, Fyn, Lyn, Hck, Lck and Blk. Lck is expressed predominantly in T cells and is localized to the inner surface of the plasma membrane. Lck is activated after T cell stimulation and is required for T-cell proliferation and IL-2 production. Aberrant expression or activation of Lck kinase has been reported in both lymphoid and nonlymphoid malignancies. In addition, inhibition of Lck has been a target to prevent lymphocyte activation and acute rejection.

Gene ID: 3932

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Transition Metal Ion Homeostasis, Positive Regulation of Endopeptidase Activity, CXCR4-mediated Signaling Events, Thromboxane A2 Receptor Signaling