LKB1 antibody (C-Term)

Catalog No. ABIN966488

There is 1 publication for this product available. The Rabbit Polyclonal anti-LKB1 antibody is suitable to detect LKB1 in samples from Human. It has been validated for WB and IHC (p).

Quick Overview for LKB1 antibody (C-Term) (ABIN966488)

Target: LKB1 (STK11) (serine/threonine Kinase 11 (STK11))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LKB1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-LKB1 Antibody

Binding Specificity: C-Term

Cross-Reactivity: Mouse (Murine), Rat (Rattus)

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Serine/threonine-protein kinase STK11 (STK11) detection. Tested with WB, IHC-P in Human, Mouse, Rat.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminal of human STK11, different from the mouse sequence by one amino acid.< br/>Immunogen was affinity purified.

Isotype: IgG

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 µg/mL.

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Sodium azide, Thimerosal (Merthiolate)

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: At -20 °C for one year. After reconstitution, at 4 °C for one month. It can also be aliquotted and stored frozen at -20 °C for a longer time.

References for anti-LKB1 antibody (ABIN966488)

Jenne, Reimann, Nezu, Friedel, Loff, Jeschke, Müller, Back, Zimmer: "Peutz-Jeghers syndrome is caused by mutations in a novel serine threonine kinase." in: Nature genetics, Vol. 18, Issue 1, pp. 38-43, (1998) (PubMed).

Target Details for LKB1

Target: LKB1 (STK11) (serine/threonine Kinase 11 (STK11))

Alternative Name: Serine/threonine-protein kinase STK11

Background: Serine/threonine kinase 11 or LKB1 is a protein kinase which in humans is encoded by the STK11 gene. The STK11/LKB1 gene, which encodes a member of the serine/threonine kinase, regulates cell polarity and functions as a tumour suppressor Smith et al. (1999) found that the mouse Lkb1 gene encodes a protein showing strong sequence similarity to human LKB1. Karuman et al. (2001) demonstrated that LKB1 physically associates with p53 (191170) and regulates specific p53-dependent apoptosis pathways. Jenne et al. (1998) determined that the STK11 gene extends over 23 kb of genomic DNA and is composed of 9 exons, which are transcribed in telomere-to-centromere direction. Smith et al. (1999) found that the mouse Lkb1 gene consists of 10 exons covering approximately 15 kb.

Pathways: AMPK Signaling, Carbohydrate Homeostasis, Regulation of Carbohydrate Metabolic Process, Warburg Effect