MCL-1 antibody

Catalog No. ABIN966543

Product Details anti-MCL-1 Antibody

Target: MCL-1 (MCL1) (Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1 (MCL1))

Reactivity: Human

Host: Please inquire

Clonality: Monoclonal

Conjugate: This MCL-1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Specificity: Ni-NTA purified recombinant protein MCL-1expressed in E. Coli strain M15.

Purification: Antibodies are purified by protein G affinity chromatography.

Isotype: IgG1

Application Details

Application Notes: Western Blot: Dilution 1: 200- 1: 1,000
IHC(P): Dilution 1: 200- 1: 1,000
IHC(F): Dilution 1: 200- 1: 1,000
ELISA: Propose dilution 1: 10,000.
Determining optimal working dilutions by titration test.

Restrictions: For Research Use only

Handling

Storage: -20 °C

References for anti-MCL-1 antibody (ABIN966543)

Marsh, Theodosopoulos, Howell, Richardson, Benn, Proos, Eng, Robinson: "Rapid mutation scanning of genes associated with familial cancer syndromes using denaturing high-performance liquid chromatography." in: Neoplasia (New York, N.Y.), Vol. 3, Issue 3, pp. 236-44, (2001) (PubMed).

Richert, Schwertfeger, Ryder, Anderson: "An atlas of mouse mammary gland development." in: Journal of mammary gland biology and neoplasia, Vol. 5, Issue 2, pp. 227-41, (2001) (PubMed).

MacLachlan, Hébert: "Statement concerning euthanasia and physician-assisted suicide. Ethics Committee of the College of Family Physicians of Canada." in: Canadian family physician Médecin de famille canadien, Vol. 46, pp. 254-6, 264-7, (2000) (PubMed).

Target Details for MCL-1

Target: MCL-1 (MCL1) (Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1 (MCL1))

Alternative Name: MCL-1 (MCL1 Products)

Background: Mcl-1 (Myeloid cell leukemia-1) is Bcl-2-related and was identified as an early-induction gene that increased in expression during the differentiation of human myeloblastic leukemia cell ML-1, or exposure to different DNA damaging agents. The level of Mcl1 is decreased in peripheral B lymphocytes undergoing apoptosis following treatment with apoptotic stimuli such as TGF-alpha 1 and forskolin. Expression of Mcl-1 is able to delay apoptosis induced by over-expression of c-myc in CHO 5AHSmyc cells. In hematopoietic FDC-P1 cells, Mcl-1 interacts with another Bcl-2-related protein, Bax, and prolongs cell viability after treatment with different apoptotic reagents.This monoclonal antibody detected a 37kd MCL1 in BCBL-1 cell lysate.

Gene ID: 4170

Pathways: MAPK Signaling