MSH2 antibody

Catalog No. ABIN966594

The Monoclonal anti-MSH2 antibody (ABIN966594) specifically detects MSH2 in WB, IHC and ELISA. The antibody is reactive with Human samples.

Quick Overview for MSH2 antibody (ABIN966594)

Target: MSH2 (Mismatch Repair Protein 2 (MSH2))

Reactivity: Human

Host: Please inquire

Clonality: Monoclonal

Conjugate: This MSH2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Product Details anti-MSH2 Antibody

Specificity: Ni-NTA purified recombinant human MSH2 expressed in E. Coli strain BL21 (DE3).

Purification: Antibodies are purified by protein G affinity chromatography.

Isotype: IgG1

Application Details

Application Notes: Western Blot: Dilution 1: 200- 1: 1,000
IHC(P): Dilution 1: 200- 1: 1,000
IHC(F): Dilution 1: 200- 1: 1,000
ELISA: Propose dilution 1: 10,000.
Determining optimal working dilutions by titration test.

Restrictions: For Research Use only

Handling

Storage: -20 °C

Target Details for MSH2

Target: MSH2 (Mismatch Repair Protein 2 (MSH2))

Alternative Name: MSH2

Background: MSH2 is a 100 kDa nuclear antigen and encodes a protein of 934 amino acids. The MSH2 gene is one of 4 known genes encoding proteins involved in the repair of mismatch nucleotides following DNA replication or repair. Mutations in the MSH2 gene contribute to the development of sporadic colorectal carcinoma. MSHS mutations are responsible for 50% of inherited non-polyposis colorectal (HNPCC). The repair of mismatch DNA is essential to maintaining the integrity of genetic information over time. An alteration of microsatellite repeats is the result of slippage owing to strand misalignment during DNA replication and is referred to as microsatellite instability (MSI). These defects in DNA repair pathways have been related to human carcinogenesis. MSH-2 is involved in the initial cognition of mismatch nucleotides during the replication mismatch repair process.

Gene ID: 4436

Pathways: DNA Damage Repair, Production of Molecular Mediator of Immune Response