NPM1 antibody

Catalog No. ABIN966704

The Mouse Monoclonal anti-NPM1 antibody has been validated for WB, IHC, IF and ELISA. It is suitable to detect NPM1 in samples from Human and Monkey. There are 2+ publications available.

Quick Overview for NPM1 antibody (ABIN966704)

Target: NPM1 (Nucleophosmin (Nucleolar phosphoprotein B23, Numatrin) (NPM1))

Reactivity: Human, Monkey

Host: Mouse

Clonality: Monoclonal

Conjugate: This NPM1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA

Clone: 7H10B9

Product Details anti-NPM1 Antibody

Purification: Ascitic fluid

Immunogen: Purified recombinant fragment of human NPM expressed in E. Coli.

Isotype: IgG1

Application Details

Application Notes: Western Bloting: 1/500 - 1/2000.
Immunohistochemistry: 1/200 - 1/1000.
Immunofluorescence: 1/200 - 1/1000.
ELISA: Propose dilution 1/10000.
Not yet tested in other applications.
Determining optimal working dilutions by titration test.

Restrictions: For Research Use only

Handling

Format: Liquid

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

References for anti-NPM1 antibody (ABIN966704)

Yoneda-Kato, Look, Kirstein, Valentine, Raimondi, Cohen, Carroll, Morris: "The t(3;5)(q25.1;q34) of myelodysplastic syndrome and acute myeloid leukemia produces a novel fusion gene, NPM-MLF1." in: Oncogene, Vol. 12, Issue 2, pp. 265-75, (1996) (PubMed).

Morris, Kirstein, Valentine, Dittmer, Shapiro, Saltman, Look: "Fusion of a kinase gene, ALK, to a nucleolar protein gene, NPM, in non-Hodgkin's lymphoma." in: Science (New York, N.Y.), Vol. 263, Issue 5151, pp. 1281-4, (1994) (PubMed).

Target Details for NPM1

Target: NPM1 (Nucleophosmin (Nucleolar phosphoprotein B23, Numatrin) (NPM1))

Alternative Name: Nucleophosmin

Background: Nucleophosmin (NPM), also named B23 or NO38, is a major nucleolar protein which is 20 times more abundant in tumor or proliferating cells than in normal resting cells. NPM has been implicated in several distinct cellular functions, including assembly and transport of ribosomes, cytoplasmic/nuclear trafficking, regulation of DNA polymerase alpha activity, centrosome duplication and molecular chaperoning activities. The NPM is also known for its fusion with the anaplastic lymphoma kinase (ALK) receptor tyrosine kinase. The NPM portion contributes to transformation by providing a dimerization domain, which results in activation of the fused kinase.
Synonyms: B23, NPM

Molecular Weight: 33kDa

Gene ID: 4869

Pathways: Ribonucleoprotein Complex Subunit Organization, Ribosome Assembly