PAK1/2/3 antibody

Catalog No. ABIN966799

This Rabbit Polyclonal antibody specifically detects PAK1/2/3 in IHC. It exhibits reactivity toward Human, Rat and Mouse. It has been mentioned in 3+ publications

Quick Overview for PAK1/2/3 antibody (ABIN966799)

Target: PAK1/2/3 (P21-Activated Kinases 1/2/3 (PAK1/2/3))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PAK1/2/3 antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Product Details anti-PAK1/2/3 Antibody

Specificity: PAK1/PAK2/PAK3 (Ab-423/402/421) antibody detects endogenous levels of total PAK1/PAK2/PAK3 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: The antiserum was produced against synthesized non-phosphopeptide derived from human PAK1around the phosphorylation site of threonine 423 (R-S-TP-M-V).

Application Details

Application Notes: IHC: 1:50-1:100.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100ug/100ul.

Buffer: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 50% glycerol

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

References for anti-PAK1/2/3 antibody (ABIN966799)

Parsons, Monypenny, Ameer-Beg, Millard, Machesky, Peter, Keppler, Schiavo, Watson, Chernoff, Zicha, Vojnovic, Ng: "Spatially distinct binding of Cdc42 to PAK1 and N-WASP in breast carcinoma cells." in: Molecular and cellular biology, Vol. 25, Issue 5, pp. 1680-95, (2005) (PubMed).

Lampugnani, Zanetti, Breviario, Balconi, Orsenigo, Corada, Spagnuolo, Betson, Braga, Dejana: "VE-cadherin regulates endothelial actin activating Rac and increasing membrane association of Tiam." in: Molecular biology of the cell, Vol. 13, Issue 4, pp. 1175-89, (2002) (PubMed).

Wang, Mazumdar, Vadlamudi, Kumar: "P21-activated kinase-1 phosphorylates and transactivates estrogen receptor-alpha and promotes hyperplasia in mammary epithelium." in: The EMBO journal, Vol. 21, Issue 20, pp. 5437-47, (2002) (PubMed).

Target Details for PAK1/2/3

Target: PAK1/2/3 (P21-Activated Kinases 1/2/3 (PAK1/2/3))

Alternative Name: PAK1/PAK2/PAK3