PARP1 antibody (full length)

Catalog No. ABIN967515

Cited in 4+ publications. The Mouse Monoclonal anti-PARP1 antibody (Clone C2-10) (ABIN967515) specifically detects PARP1 in WB and IF. The antibody is reactive with Human, Mouse, Cow and Hamster samples.

Quick Overview for PARP1 antibody (full length) (ABIN967515)

Target: PARP1 (Poly (ADP-Ribose) Polymerase 1 (PARP1))

Reactivity: Human, Mouse, Cow, Hamster

Host: Mouse

Clonality: Monoclonal

Conjugate: This PARP1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Clone: C2-10

Product Details anti-PARP1 Antibody

Binding Specificity: full length

Cross-Reactivity: Mouse (Murine), Hamster, Human

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Purification: Ascites

Immunogen: Cow PARP (full-length, thymus derived)

Isotype: IgG1

Application Details

Application Notes: This antibody is prepared as an ascites solution. For western blot analysis, investigators are highly encouraged to titrate the antibody for optimal performance. A titration range between 1:2000 to 1:4000 is suggested.

Comment:

Related Products: ABIN967540, ABIN967299, ABIN967389

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4° C.

References for anti-PARP1 antibody (ABIN967515)

Patel, Gores, Kaufmann: "The role of proteases during apoptosis." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 10, Issue 5, pp. 587-97, (1996) (PubMed).

Tewari, Quan, ORourke, Desnoyers, Zeng, Beidler, Poirier, Salvesen, Dixit: "Yama/CPP32 beta, a mammalian homolog of CED-3, is a CrmA-inhibitable protease that cleaves the death substrate poly(ADP-ribose) polymerase." in: Cell, Vol. 81, Issue 5, pp. 801-9, (1995) (PubMed).

Lamarre, Talbot, de Murcia, Laplante, Leduc, Mazen, Poirier: "Structural and functional analysis of poly(ADP ribose) polymerase: an immunological study." in: Biochimica et biophysica acta, Vol. 950, Issue 2, pp. 147-60, (1988) (PubMed).

Lamarre, Talbot, Leduc, Muller, Poirier: "Production and characterization of monoclonal antibodies specific for the functional domains of poly(ADP-ribose) polymerase." in: Biochemistry and cell biology = Biochimie et biologie cellulaire, Vol. 64, Issue 4, pp. 368-76, (1986) (PubMed).

Target Details for PARP1

Target: PARP1 (Poly (ADP-Ribose) Polymerase 1 (PARP1))

Alternative Name: PARP

Background: PARP [Poly(ADP-Ribose) Polymerase] is a 113 kDa nuclear chromatin associated enzyme that catalyzes the transfer of ADP-ribose units from NAD+ to a variety of nuclear proteins including topoisomerases, histones, and PARP itself. The catalytic activity of PARP is increased in nonapoptotic cells following DNA damage, and PARP is thought to play an important role in mediating the normal cellular reponse to DNA damage and is a target of the caspase protease activity associated with apoptosis. During apoptosis, PARP is cleaved from a 113 kDa intact form into 89 kDa and 24 kDa fragments. This process separates the amino-terminal DNA-binding domain of the enzyme from the C-terminal catalytic domain resulting in the loss of normal PARP function. Although the role of PARP in apoptosis remains to be elucidated, PARP cleavage is considered to be a marker of apoptosis. This antibody has been reported to recognize an epitope located within the DNA-binding domain of the enzyme.
Synonyms: Poly (ADP-Ribose) Polymerase

Molecular Weight: 113 kDa (full-length), 89 kDa (cleaved)

Pathways: Apoptosis, Caspase Cascade in Apoptosis, DNA Damage Repair, Production of Molecular Mediator of Immune Response, Maintenance of Protein Location