Annexin A2 antibody (AA 123-328)

Catalog No. ABIN967722

Cited in 5+ publications. The Mouse Monoclonal anti-Annexin A2 antibody (Clone 5-Annexin II) (ABIN967722) specifically detects Annexin A2 in WB, IF, IP and IHC (f). The antibody is reactive with Human, Mouse, Rat, Dog and Chicken samples.

Quick Overview for Annexin A2 antibody (AA 123-328) (ABIN967722)

Target: Annexin A2 (ANXA2)

Reactivity: Human, Mouse, Rat, Dog, Chicken

Host: Mouse

Clonality: Monoclonal

Conjugate: This Annexin A2 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: 5-Annexin II

Product Details anti-Annexin A2 Antibody

Binding Specificity: AA 123-328

Cross-Reactivity: Chicken, Dog (Canine), Mouse (Murine), Rat (Rattus)

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human Annexin II aa. 123-328

Isotype: IgG1

Application Details

Comment:

Related Products: ABIN968533, ABIN967389

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store undiluted at -20° C.

References for anti-Annexin A2 antibody (ABIN967722)

Hansen, Ehrlich, Nelson: "Molecular mechanism for orienting membrane and actin dynamics to nascent cell-cell contacts in epithelial cells." in: The Journal of biological chemistry, Vol. 277, Issue 47, pp. 45371-6, (2002) (PubMed).

Kwon, Caplan, Filipenko, Choi, Fitzpatrick, Zhang, Waisman: "Identification of annexin II heterotetramer as a plasmin reductase." in: The Journal of biological chemistry, Vol. 277, Issue 13, pp. 10903-11, (2002) (PubMed).

Choi, Fitzpatrick, Filipenko, Fogg, Kassam, Magliocco, Waisman: "Regulation of plasmin-dependent fibrin clot lysis by annexin II heterotetramer." in: The Journal of biological chemistry, Vol. 276, Issue 27, pp. 25212-21, (2001) (PubMed).

Jacovina, Zhong, Khazanova, Lev, Deora, Hajjar: "Neuritogenesis and the nerve growth factor-induced differentiation of PC-12 cells requires annexin II-mediated plasmin generation." in: The Journal of biological chemistry, Vol. 276, Issue 52, pp. 49350-8, (2001) (PubMed).

Chasserot-Golaz, Vitale, Sagot, Delouche, Dirrig, Pradel, Henry, Aunis, Bader: "Annexin II in exocytosis: catecholamine secretion requires the translocation of p36 to the subplasmalemmal region in chromaffin cells." in: The Journal of cell biology, Vol. 133, Issue 6, pp. 1217-36, (1996) (PubMed).

Target Details for Annexin A2

Target: Annexin A2 (ANXA2)

Alternative Name: Annexin II

Background: Annexin II (p36), originally discovered as a substrate for the src oncogene, is one of the most studied members of the annexin family of calcium-dependent phospholipid-binding proteins. It exists in cells as either a monomer or a heterotetramer complexed with p11, an S100-related protein. In vitro, it has been found that unphosphorylated annexin II is capable of causing aggregation of chromaffin granules, and, upon phosphorylation, induces fusion of the granules' membranes. A similiar phenomenon may occur in the final steps of exocytosis after cytoplasmic annexin translocates to the subplasmalemmal region and is phosphorylated by protein kinase C. However, other studies refute annexin II's involvement in exocytosis, and a conclusive role for annexin II in membrane trafficking remains debated. This antibody is routinely tested by western blot analysis.
Synonyms: Lipocortin II, Calpactin I

Molecular Weight: 36 kDa

Pathways: S100 Proteins