Annexin A2 antibody (AA 1-97)

Catalog No. ABIN967724

There are 5+ publications for this product available. The Mouse Monoclonal anti-Annexin A2 antibody is suitable to detect Annexin A2 in samples from Human, Mouse, Rat, Dog and Chicken. It has been validated for WB, IHC, IF and IP.

Quick Overview for Annexin A2 antibody (AA 1-97) (ABIN967724)

Target: Annexin A2 (ANXA2)

Reactivity: Human, Mouse, Rat, Dog, Chicken

Host: Mouse

Clonality: Monoclonal

Conjugate: This Annexin A2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)

Clone: 148-Annexin II Light Chain

Product Details anti-Annexin A2 Antibody

Binding Specificity: AA 1-97

Cross-Reactivity: Dog (Canine), Chicken, Human

No Cross-Reactivity: Rat (Rattus), Mouse (Murine)

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Cow Annexin II Light Chain aa. 1-97

Isotype: IgG1

Application Details

Comment:

Related Products: ABIN968536, ABIN967389

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store undiluted at -20°C.

References for anti-Annexin A2 antibody (ABIN967724)

Huang, Pawliczak, Cowan, Gladwin, Madara, Logun, Shelhamer: "Epidermal growth factor induces p11 gene and protein expression and down-regulates calcium ionophore-induced arachidonic acid release in human epithelial cells." in: The Journal of biological chemistry, Vol. 277, Issue 41, pp. 38431-40, (2002) (PubMed).

Kwon, Caplan, Filipenko, Choi, Fitzpatrick, Zhang, Waisman: "Identification of annexin II heterotetramer as a plasmin reductase." in: The Journal of biological chemistry, Vol. 277, Issue 13, pp. 10903-11, (2002) (PubMed).

Choi, Fitzpatrick, Filipenko, Fogg, Kassam, Magliocco, Waisman: "Regulation of plasmin-dependent fibrin clot lysis by annexin II heterotetramer." in: The Journal of biological chemistry, Vol. 276, Issue 27, pp. 25212-21, (2001) (PubMed).

Harder, Kellner, Parton, Gruenberg: "Specific release of membrane-bound annexin II and cortical cytoskeletal elements by sequestration of membrane cholesterol." in: Molecular biology of the cell, Vol. 8, Issue 3, pp. 533-45, (1997) (PubMed).

Regnouf, Sagot, Delouche, Devilliers, Cartaud, Henry, Pradel: ""In vitro" phosphorylation of annexin 2 heterotetramer by protein kinase C. Comparative properties of the unphosphorylated and phosphorylated annexin 2 on the aggregation and fusion of chromaffin granule membranes." in: The Journal of biological chemistry, Vol. 270, Issue 45, pp. 27143-50, (1995) (PubMed).

Target Details for Annexin A2

Target: Annexin A2 (ANXA2)

Alternative Name: Annexin II

Background: Annexin II often exists as a heterotetramer complexed with an 11kDa light chain. This p11 subunit is related to the S100 family of calcium-binding proteins but mutations have rendered it unable to bind calcium. The p11 protein binds to the amino terminus of the annexin II monomer, but not to other annexins. It may regulate the function of annexin II, as the tetrameric form seems to have a higher calcium and phospholipid-binding affinity than the monomeric annexin II. In the cell, p11 has been localized to the cytoplasmic face of the plasma membrane where the annexin II tetramer is known to exist.

Molecular Weight: 11 kDa

Pathways: S100 Proteins