SNCA antibody (AA 15-123)

Catalog No. ABIN968177

Product Details anti-SNCA Antibody

Target: SNCA (Synuclein, alpha (SNCA))

Binding Specificity: AA 15-123

Reactivity: Human, Rat, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This SNCA antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Cross-Reactivity: Human, Mouse (Murine)

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Rat Synuclein-1 aa. 15-123

Clone: 42-alpha

Isotype: IgG1

Application Details

Comment:

Related Products: ABIN968545, ABIN967389

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store undiluted at -20° C.

References for anti-SNCA antibody (ABIN968177)

Hansen, Björklund, Petit, Lundblad, Murmu, Brundin, Li: "A novel α-synuclein-GFP mouse model displays progressive motor impairment, olfactory dysfunction and accumulation of α-synuclein-GFP." in: Neurobiology of disease, Vol. 56, pp. 145-55, (2014) (PubMed).

Fauss, Motter, Dofiles, Rodrigues, You, Diep, Yang, Seto, Tanaka, Baker, Bergeron: "Development of an enzyme-linked immunosorbent assay (ELISA) to measure the level of tyrosine hydroxylase protein in brain tissue from Parkinson's disease models." in: Journal of neuroscience methods, Vol. 215, Issue 2, pp. 245-57, (2013) (PubMed).

Olzmann, Bordelon, Muly, Rees, Levey, Li, Chin: "Selective enrichment of DJ-1 protein in primate striatal neuronal processes: implications for Parkinson's disease." in: The Journal of comparative neurology, Vol. 500, Issue 3, pp. 585-99, (2007) (PubMed).

Liu, Fallon, Lashuel, Liu, Lansbury: "The UCH-L1 gene encodes two opposing enzymatic activities that affect alpha-synuclein degradation and Parkinson's disease susceptibility." in: Cell, Vol. 111, Issue 2, pp. 209-18, (2002) (PubMed).

Jo, McLaurin, Yip, St George-Hyslop, Fraser: "alpha-Synuclein membrane interactions and lipid specificity." in: The Journal of biological chemistry, Vol. 275, Issue 44, pp. 34328-34, (2000) (PubMed).

Ostrerova-Golts, Petrucelli, Hardy, Lee, Farer, Wolozin: "The A53T alpha-synuclein mutation increases iron-dependent aggregation and toxicity." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 20, Issue 16, pp. 6048-54, (2000) (PubMed).

van der Putten, Wiederhold, Probst, Barbieri, Mistl, Danner, Kauffmann, Hofele, Spooren, Ruegg, Lin, Caroni, Sommer, Tolnay, Bilbe: "Neuropathology in mice expressing human alpha-synuclein." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 20, Issue 16, pp. 6021-9, (2000) (PubMed).

Maroteaux, Campanelli, Scheller: "Synuclein: a neuron-specific protein localized to the nucleus and presynaptic nerve terminal." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 8, Issue 8, pp. 2804-15, (1988) (PubMed).

Target Details for SNCA

Target: SNCA (Synuclein, alpha (SNCA))

Alternative Name: alpha -Synuclein (SNCA Products)

Synonyms: snca antibody, MGC64356 antibody, LOC619283 antibody, NACP antibody, alphaSYN antibody, PARK1 antibody, PARK4 antibody, PD1 antibody, synuclein alpha L homeolog antibody, alpha-synuclein antibody, synuclein alpha antibody, synuclein, alpha antibody, snca.L antibody, LOC619283 antibody, SNCA antibody, Snca antibody

Background: Synuclein cDNA was isolated from Torpedo californica and found to encode a 143 amino acid neuron-specific protein. In addition, a rat brain cDNA library yielded several clones that encode different proteins with homology to Torpedo Synuclein. A 140 amino acid protein in rat is referred to as Synuclein-1. In situ hybridization shows synuclein mRNAs in discrete areas of rat brain, notably the hippocampus. In humans, two synuclein proteins of 140 and 134 amino acids were sequenced that exhibit a high degree of homology with each other (61% identity) and with previously described Synuclein proteins. The 140 amino acid protein (alpha-Synuclein) is the human homologue of rat Synuclein-1 and is identical to the non-amyloid-beta component precursor (NACP), a presynaptic protein involved in amyloidogenesis in Alzheimer's disease (AD). The 134 amino acid protein (beta-Synuclein) is the human homologue of bovine phosphoneuroprotein. These proteins are expressed in brain, primarily in presynaptic nerve terminals. Although the exact function of the Synucleins has not been determined, they have been linked to the prominent neurodegenerative disorders AD and Parkinson's disease. This antibody is routinely tested by western blot analysis.

Molecular Weight: 19 kDa

Pathways: Synaptic Membrane, Regulation of G-Protein Coupled Receptor Protein Signaling, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Platelet-derived growth Factor Receptor Signaling, Negative Regulation of Transporter Activity, Regulation of long-term Neuronal Synaptic Plasticity