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MSH6 antibody (AA 225-333)

MSH6 Reactivity: Human, Mouse, Rat, Dog WB, IF Host: Mouse Monoclonal 44-MSH6 unconjugated
Catalog No. ABIN968248
  • Target See all MSH6 Antibodies
    MSH6 (MutS Homolog 6 (E. Coli) (MSH6))
    Binding Specificity
    • 15
    • 8
    • 5
    • 4
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 225-333
    Reactivity
    • 79
    • 35
    • 29
    • 9
    • 3
    • 2
    • 1
    • 1
    • 1
    Human, Mouse, Rat, Dog
    Host
    • 59
    • 17
    • 3
    Mouse
    Clonality
    • 46
    • 33
    Monoclonal
    Conjugate
    • 47
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    This MSH6 antibody is un-conjugated
    Application
    • 54
    • 27
    • 25
    • 21
    • 14
    • 13
    • 12
    • 8
    • 8
    • 7
    • 6
    • 6
    • 4
    • 3
    • 3
    • 2
    • 1
    • 1
    Western Blotting (WB), Immunofluorescence (IF)
    Cross-Reactivity
    Dog (Canine), Mouse (Murine), Rat (Rattus)
    Characteristics
    1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
    2. Please refer to us for technical protocols.
    3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
    4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
    Purification
    The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
    Immunogen
    Human MSH6 aa. 225-333
    Clone
    44-MSH6
    Isotype
    IgG1
    Top Product
    Discover our top product MSH6 Primary Antibody
  • Comment

    Related Products: ABIN968533, ABIN967389

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    250 μg/mL
    Buffer
    Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    -20 °C
    Storage Comment
    Store undiluted at -20° C.
  • Kariola, Otway, Lönnqvist, Raevaara, Macrae, Vos, Kohonen-Corish, Hofstra, Nyström-Lahti: "Two mismatch repair gene mutations found in a colon cancer patient--which one is pathogenic?" in: Human genetics, Vol. 112, Issue 2, pp. 105-9, (2003) (PubMed).

    Saitoh, Pizzi, Wang: "Perturbation of SUMOlation enzyme Ubc9 by distinct domain within nucleoporin RanBP2/Nup358." in: The Journal of biological chemistry, Vol. 277, Issue 7, pp. 4755-63, (2002) (PubMed).

    Humbert, Hermine, Hernandez, Bouget, Selves, Laurent, Salles, Lautier: "Implication of protein kinase C in the regulation of DNA mismatch repair protein expression and function." in: The Journal of biological chemistry, Vol. 277, Issue 20, pp. 18061-8, (2002) (PubMed).

    Christmann, Kaina: "Nuclear translocation of mismatch repair proteins MSH2 and MSH6 as a response of cells to alkylating agents." in: The Journal of biological chemistry, Vol. 275, Issue 46, pp. 36256-62, (2000) (PubMed).

    Palombo, Gallinari, Iaccarino, Lettieri, Hughes, DArrigo, Truong, Hsuan, Jiricny: "GTBP, a 160-kilodalton protein essential for mismatch-binding activity in human cells." in: Science (New York, N.Y.), Vol. 268, Issue 5219, pp. 1912-4, (1995) (PubMed).

  • Target
    MSH6 (MutS Homolog 6 (E. Coli) (MSH6))
    Alternative Name
    MSH6 (MSH6 Products)
    Synonyms
    GTBP antibody, GTMBP antibody, HNPCC5 antibody, HSAP antibody, p160 antibody, cb326 antibody, wu:fb36c12 antibody, LOC100227810 antibody, AU044881 antibody, AW550279 antibody, Gtmbp antibody, mutS homolog 6 antibody, mutS homolog 6 (E. coli) antibody, DNA mismatch repair protein antibody, MSH6 antibody, msh6 antibody, Msh6 antibody
    Background
    DNA mismatch repair in bacteria is carried out by the MutL, MutH, and MutS proteins. Initial binding of MutS to the mismatched DNA is followed by binding of the MutH endonuclease and MutL. Together these proteins form a complex that mediates excision repair. Mutations or deficiencies of any of these bacterial proteins results in a mutator phenotype that is characterized by genetic instability. MSH2, MSH3, and MSH6 are human homologs of MutS, while MLH1, PMS1, and PMS2 are homologs of MutL. As a heterodimer with MSH2, MSH6 binds to DNA containing G/T mismatches. The MSH2-MSH6 complex recognizes single-base mispairs and insertion/deletion loops. Binding of this complex induces conformational changes in the DNA that lead to the binding of an MLH-PMS1 complex and excision repair. Mutations in the human genes are associated with hereditary nonpolyposis colon cancer (HNPCC), a common hereditary disease in humans. HNPCC is characterized by frequent microsatellite mutations that arise from somatic mutation due to a replication error (RER+) phenotype. This phenotype is analogous to the bacterial system and is directly linked to DNA mismatch repair deficiencies. This antibody is routinely tested by western blot analysis.
    Synonyms: GTBP
    Molecular Weight
    160 kDa
    Pathways
    DNA Damage Repair, Chromatin Binding, Production of Molecular Mediator of Immune Response
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