HIF1A antibody (AA 610-727)

Catalog No. ABIN968275

This Mouse Monoclonal antibody specifically detects HIF1A in WB and IF. It exhibits reactivity toward Human. It has been mentioned in 6+ publications

Quick Overview for HIF1A antibody (AA 610-727) (ABIN968275)

Target: HIF1A (Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This HIF1A antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Clone: 54-HIF

Product Details anti-HIF1A Antibody

Binding Specificity: AA 610-727

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human HIF-1alpha aa. 610-727

Isotype: IgG1 kappa

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store undiluted at -20°C.

References for anti-HIF1A antibody (ABIN968275)

Fallone, Britton, Nieto, Salles, Muller: "ATR controls cellular adaptation to hypoxia through positive regulation of hypoxia-inducible factor 1 (HIF-1) expression." in: Oncogene, Vol. 32, Issue 37, pp. 4387-96, (2013) (PubMed).

Eckard, Dai, Wu, Jian, Yang, Chen, Costa, Frenkel, Huang: "Effects of cellular iron deficiency on the formation of vascular endothelial growth factor and angiogenesis. Iron deficiency and angiogenesis." in: Cancer cell international, Vol. 10, pp. 28, (2010) (PubMed).

Arsham, Plas, Thompson, Simon: "Phosphatidylinositol 3-kinase/Akt signaling is neither required for hypoxic stabilization of HIF-1 alpha nor sufficient for HIF-1-dependent target gene transcription." in: The Journal of biological chemistry, Vol. 277, Issue 17, pp. 15162-70, (2002) (PubMed).

Sánchez-Elsner, Botella, Velasco, Langa, Bernabéu: "Endoglin expression is regulated by transcriptional cooperation between the hypoxia and transforming growth factor-beta pathways." in: The Journal of biological chemistry, Vol. 277, Issue 46, pp. 43799-808, (2002) (PubMed).

Wiesener, Münchenhagen, Berger, Morgan, Roigas, Schwiertz, Jürgensen, Gruber, Maxwell, Löning, Frei, Maher, Gröne, Eckardt: "Constitutive activation of hypoxia-inducible genes related to overexpression of hypoxia-inducible factor-1alpha in clear cell renal carcinomas." in: Cancer research, Vol. 61, Issue 13, pp. 5215-22, (2001) (PubMed).

Srinivas, Leshchinsky, Sang, King, Minchenko, Caro: "Oxygen sensing and HIF-1 activation does not require an active mitochondrial respiratory chain electron-transfer pathway." in: The Journal of biological chemistry, Vol. 276, Issue 25, pp. 21995-8, (2001) (PubMed).

Target Details for HIF1A

Target: HIF1A (Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A))

Alternative Name: HIF-1alpha

Background: Molecular oxygen (O2) is essential for mammalian metabolic processes such as oxidative phosphorylation. Thus, survival depends upon instantaneous transcriptional modulation of genes that maintain O2 homeostasis. Transcriptional control of several of these genes is mediated by hypoxia-inducible factor 1 (HIF-1). HIF-1 is a heterodimer whose alpha and beta subunits are members of the PAS family of basic helix-loop-helix (bHLH) transcription factors. Common structural features of these proteins are an N-terminal bHLH DNA-binding domain and multiple PAS domains that confer dimerization ability and target gene specificity. Members diverge in their C-terminal regions. HIF-1beta is also known as the arylhydrocarbon nuclear translocator which is part of the functional dioxin receptor. However, HIF-1alpha functions exclusively to mediate responses to O2 deprivation. It contains C-terminal and internal transactivation domains. Although HIF-1 aproteinlevels increase during hypoxia, it is unstable in the presence of O2 due to an oxygen-dependent degradation domain (ODD) that targets it for ubiquitination. Thus, HIF-1alpha is essential for functional HIF-1 to mediate gene transcription in response to hypoxia. This antibody is routinely tested by western blot analysis.

Molecular Weight: 120 kDa

Pathways: Positive Regulation of Peptide Hormone Secretion, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Transition Metal Ion Homeostasis, Tube Formation, Regulation of Carbohydrate Metabolic Process, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals, Warburg Effect