FUS antibody (AA 2-117)

Catalog No. ABIN968509

Product Details anti-FUS Antibody

Target: FUS (Fused in Sarcoma (FUS))

Binding Specificity: AA 2-117

Reactivity: Human, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This FUS antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Cross-Reactivity: Rat (Rattus)

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human TLS aa. 2-117.

Clone: 15-TLS

Isotype: IgG1

Application Details

Comment:

Related Products: ABIN968537, ABIN967389

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store undiluted at -20° C.

References for anti-FUS antibody (ABIN968509)

Hallier, Lerga, Barnache, Tavitian, Moreau-Gachelin: "The transcription factor Spi-1/PU.1 interacts with the potential splicing factor TLS." in: The Journal of biological chemistry, Vol. 273, Issue 9, pp. 4838-42, (1998) (PubMed).

Morohoshi, Ootsuka, Arai, Ichikawa, Mitani, Munakata, Ohki: "Genomic structure of the human RBP56/hTAFII68 and FUS/TLS genes." in: Gene, Vol. 221, Issue 2, pp. 191-8, (1998) (PubMed).

Powers, Mathur, Raaka, Ron, Samuels: "TLS (translocated-in-liposarcoma) is a high-affinity interactor for steroid, thyroid hormone, and retinoid receptors." in: Molecular endocrinology (Baltimore, Md.), Vol. 12, Issue 1, pp. 4-18, (1998) (PubMed).

Yang, Embree, Tsai, Hickstein: "Oncoprotein TLS interacts with serine-arginine proteins involved in RNA splicing." in: The Journal of biological chemistry, Vol. 273, Issue 43, pp. 27761-4, (1998) (PubMed).

Zinszner, Sok, Immanuel, Yin, Ron: "TLS (FUS) binds RNA in vivo and engages in nucleo-cytoplasmic shuttling." in: Journal of cell science, Vol. 110 ( Pt 15), pp. 1741-50, (1997) (PubMed).

Target Details for FUS

Target: FUS (Fused in Sarcoma (FUS))

Alternative Name: TLS (FUS Products)

Synonyms: ALS6 antibody, ETM4 antibody, FUS1 antibody, HNRNPP2 antibody, POMP75 antibody, TLS antibody, D430004D17Rik antibody, D930039C12Rik antibody, Fus1 antibody, Tls antibody, FUS/TLS antibody, FUS RNA binding protein antibody, fused in sarcoma antibody, FUS antibody, Fus antibody

Target Type: Viral Protein

Background: Chromosomal translocations in leukemias and malignancies of non-hematopoietic tissues usually result in the fusion of genes and the production of novel fusion proteins. Translocated in liposarcoma (TLS/FUS) was identified through its fusion to the transcription factor CHOP in human myxoid liposarcoma. In addition, the fusion of TLS to the transcription factor ERG is involved in human acute myeloid leukemia. TLS contains an N-terminal Gln-, Ser-, and Tyr-rich region (QSY) that is thought to be a potent transactivator when fused with transcription factors. In its C-terminus, TLS contains a ribonucleoprotein consensus sequence (RNP-CS) and Arg-Gly-Gly (RGG) repeats that have been implicated in RNA binding. This feature allows it to function in shuttling of RNA from the nucleus. TLS also interacts with Ser-Arg proteins that regulate RNA splicing. Interestingly, TLS also interacts with the DNA binding domains of the estrogen, thyroid hormone, and glucocorticoid receptors. Thus, TLS is a multifunctional protein that is involved in RNA transport, nuclear receptor function, and gene transactivation when fused with transcription factors. This antibody is routinely tested by western blot analysis.

Molecular Weight: 65 kDa