CYBB antibody (AA 450-556)

Catalog No. ABIN968524

Product Details anti-CYBB Antibody

Target: CYBB (Cytochrome B-245, beta Polypeptide (CYBB))

Binding Specificity: AA 450-556

Reactivity: Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This CYBB antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Cross-Reactivity: Rat (Rattus)

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Mouse gp91[phox] aa. 450-556

Clone: 53-gp91[phox]

Isotype: IgG1

Application Details

Comment:

Related Products: ABIN968555, ABIN967389, ABIN967918, ABIN968245

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store undiluted at -20°C.

References for anti-CYBB antibody (ABIN968524)

Jacobsen, Skalnik: "YY1 binds five cis-elements and trans-activates the myeloid cell-restricted gp91(phox) promoter." in: The Journal of biological chemistry, Vol. 274, Issue 42, pp. 29984-93, (1999) (PubMed).

Suzuki, Kumatori, Haagen, Fujii, Sadat, Jun, Tsuji, Roos, Nakamura: "PU.1 as an essential activator for the expression of gp91(phox) gene in human peripheral neutrophils, monocytes, and B lymphocytes." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, Issue 11, pp. 6085-90, (1998) (PubMed).

Yu, Quinn, Cross, Dinauer: "Gp91(phox) is the heme binding subunit of the superoxide-generating NADPH oxidase." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, Issue 14, pp. 7993-8, (1998) (PubMed).

Zhen, Yu, Dinauer: "Probing the role of the carboxyl terminus of the gp91phox subunit of neutrophil flavocytochrome b558 using site-directed mutagenesis." in: The Journal of biological chemistry, Vol. 273, Issue 11, pp. 6575-81, (1998) (PubMed).

Target Details for CYBB

Target: CYBB (Cytochrome B-245, beta Polypeptide (CYBB))

Alternative Name: gp91 phox (CYBB Products)

Synonyms: zgc:64144 antibody, cgd antibody, gp91-1 antibody, gp91-phox antibody, gp91phox antibody, nox2 antibody, NOX2 antibody, cybb antibody, NOX1 antibody, AMCBX2 antibody, CGD antibody, GP91-1 antibody, GP91-PHOX antibody, GP91PHOX antibody, p91-PHOX antibody, Nox2 antibody, C88302 antibody, CGD91-phox antibody, Cgd antibody, Cyd antibody, Gp91-phox antibody, CYBB antibody, cytochrome b-245, beta polypeptide (chronic granulomatous disease) antibody, cytochrome b-245, beta polypeptide antibody, cytochrome b-245 heavy chain antibody, cytochrome b-245, beta polypeptide L homeolog antibody, cytochrome b-245 beta polypeptide antibody, cytochrome b-245 beta chain antibody, NADPH oxidase heavy chain subunit antibody, cybb antibody, CYBB antibody, cybb.L antibody, Cybb antibody, GP91-PHOX antibody

Background: Although dormant in resting granulocytes, macrophages, and B lymphocytes, the neutrophil respiratory burst oxidase (NADPH-oxidase) generates superoxide and secondary oxygen-derived toxic products in response to bacteria or a variety of soluble stimuli. It is an integral membrane cytochrome, b558, which consists of two subunits, gp91[phox] and p21[phox]. Upon stimulation, cytochrome b558 forms a complex with cytosolic proteins, p67[phox], p47[phox], p40[phox], and rac2 and produces superoxide anions in a NADPH-dependent manner. In chronic granulomatous disease (CGD), severe recurrent bacterial and fungal infections result from defective NADPH-oxidase activity. The majority of CGD cases are caused by a defective gp91[phox] gene. gp91[phox], implicated as a docking site for p47[phox], is membrane glycoprotein with multiple N-terminal hydrophobic domains and a hydrophilic C-terminus. The expression of gp91[phox] is restricted to terminally differentiated phagocytes and B lymphocytes. This cell type- and developmental stage-specific expression may be controlled by transcriptional activators, such as PU.1 and YY1, and transcriptional repressors, such as CCAAT displacement protein (CDP). Endogenous mouse gp91phox shows 58 kDa band, instead of 91 kDa observed in human. Both mouse and human deglycosylated gp91 are 54 kDa. This difference may be due to less glycosylation sites in the mouse sequence.

Molecular Weight: 58 kDa