EZH2 antibody (AA 156-256)

Catalog No. ABIN968907

Cited in 2+ publications. The Mouse Monoclonal anti-EZH2 antibody (Clone 11-EZH2) (ABIN968907) specifically detects EZH2 in WB and IF. The antibody is reactive with Human, Mouse, Rat, Dog and Chicken samples.

Quick Overview for EZH2 antibody (AA 156-256) (ABIN968907)

Target: EZH2 (Enhancer of Zeste Homolog 2 (EZH2))

Reactivity: Human, Mouse, Rat, Dog, Chicken

Host: Mouse

Clonality: Monoclonal

Conjugate: This EZH2 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Clone: 11-EZH2

Product Details anti-EZH2 Antibody

Binding Specificity: AA 156-256

Cross-Reactivity: Dog (Canine), Rat (Rattus), Mouse (Murine), Chicken

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human EZH2 recombinant protein aa. 156-256

Isotype: IgG1

Application Details

Comment:

Related Products: ABIN968537, ABIN967389

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store undiluted at -20°C.

References for anti-EZH2 antibody (ABIN968907)

Raaphorst, Otte, van Kemenade, Blokzijl, Fieret, Hamer, Satijn, Meijer: "Distinct BMI-1 and EZH2 expression patterns in thymocytes and mature T cells suggest a role for Polycomb genes in human T cell differentiation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 166, Issue 10, pp. 5925-34, (2001) (PubMed).

van Kemenade, Raaphorst, Blokzijl, Fieret, Hamer, Satijn, Otte, Meijer: "Coexpression of BMI-1 and EZH2 polycomb-group proteins is associated with cycling cells and degree of malignancy in B-cell non-Hodgkin lymphoma." in: Blood, Vol. 97, Issue 12, pp. 3896-901, (2001) (PubMed).

Target Details for EZH2

Target: EZH2 (Enhancer of Zeste Homolog 2 (EZH2))

Alternative Name: EZH2

Background: The 11/EZH2 monoclonal antibody specifically binds to the methyltransferase, EZH2 (Enhancer of Zeste Homolog 2). EZH2 is a human homologue of Drosophila's Enhancer of zeste gene, an important regulator of homeobox gene expression. The EZH2 protein has a predicted molecular weight of ~85 kDa. EZH2 is a member of the Polycomb group (PcG) of proteins that are essential for the maintenance, but not initiation, of the transcriptionally repressed state of certain developmental genes. PcG proteins are a structurally diverse group of proteins with conserved functions from fly to human cells. PcG family proteins form multimeric complexes that regulate the expression of genes involved in cell cycle, DNA repair and differentiation. Specifically, EZH2 is a core enzymatic component of PRC2 (polycomb repressive complex 2). EZH2 is expressed in some lymph node follicular T cells and B cells. Thymocytes differentially express EZH2 at various stages during T-cell maturation. EZH2 interacts with multiple signaling proteins, including Vav, that are involved in lymphocyte development and activation. It is highly expressed in a variety of tumors including lymphomas as well as breast and prostate cancers. EZH2 is important in the self renewal and proliferation of numerous stem cell types including fetal hematopoietic stem cells, muscle satellite cells, hepatic stem/progenitor cells, neural stem cells, basal cell progenitors in the developing epidermis, embryonic stem cells, and some cancer stem cells.

Molecular Weight: 91 kDa

Pathways: Retinoic Acid Receptor Signaling Pathway, Regulation of Muscle Cell Differentiation