Aurora A antibody

Catalog No. ABIN968971

This Mouse Monoclonal antibody specifically detects Aurora A in WB, ELISA, ICC and FACS. It exhibits reactivity toward Human, Rat and Monkey and has been mentioned in 2+ publications.

Quick Overview for Aurora A antibody (ABIN968971)

Target: Aurora A (AURKA) (Aurora Kinase A (AURKA))

Reactivity: Human, Rat, Monkey

Host: Mouse

Clonality: Monoclonal

Conjugate: This Aurora A antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Flow Cytometry (FACS)

Clone: 1F8

Product Details anti-Aurora A Antibody

Purpose: AURKA Antibody

Purification: Ascitic fluid

Immunogen: Purified recombinant fragment of human AURKA expressed in E. Coli.

Isotype: IgG1

Application Details

Application Notes:

ELISA: 1/10000

FCM: 1/200 - 1/400

ICC: 1/200 - 1/1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Ascitic fluid containing 0.03 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

References for anti-Aurora A antibody (ABIN968971)

Veerakumarasivam, Goldstein, Saeb-Parsy, Scott, Warren, Thorne, Mills, Venkitaraman, Neal, Kelly: "AURKA overexpression accompanies dysregulation of DNA-damage response genes in invasive urothelial cell carcinoma." in: Cell cycle (Georgetown, Tex.), Vol. 7, Issue 22, pp. 3525-33, (2008) (PubMed).

De Luca, Brunetto, Asteriti, Giubettini, Lavia, Guarguaglini: "Aurora-A and ch-TOG act in a common pathway in control of spindle pole integrity." in: Oncogene, Vol. 27, Issue 51, pp. 6539-49, (2008) (PubMed).

Target Details for Aurora A

Target: Aurora A (AURKA) (Aurora Kinase A (AURKA))

Alternative Name: AURKA

Background: Aurora A plays a role in cell cycle regulation during anaphase and/or telophase, in relation to the function of the centrosome/spindle pole region during chromosome segregation. Aurora A plays a key role during tumor development and progression and is overexpressed in many human cancers including breast, ovarian and colorectal. Aurora A is viewed as a potential target for anticancer drug treatment.Tissue specificity: Highly expressed in testis and weakly in skeletal muscle, thymus and spleen. Also highly expressed in colon, ovarian, prostate, neuroblastoma, breast and cervical cancer cell lines.

Molecular Weight: 48 kDa

UniProt: O14965

Pathways: Cell Division Cycle, Asymmetric Protein Localization