CEBPA antibody (CCAAT/enhancer Binding Protein (C/EBP), alpha)

Details for Product anti-CEBPA Antibody No. ABIN969048
Antigen
Reactivity
Human
191
141
129
10
9
6
6
5
4
4
3
2
1
1
Host
Mouse
189
14
3
1
Clonality (Clone)
Monoclonal ()
Conjugate
This CEBPA antibody is un-conjugated
10
7
7
7
7
7
7
4
4
2
2
2
1
1
1
1
1
Application
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
153
84
46
33
31
15
9
5
3
2
2
2
1
1
1
Options
Immunogen Synthesized peptide of human CEBPA (AA C-RKSRDKAKRNVETKV).
Sequence RKSRDKAKRN VETKV
Clone 5B7
Isotype IgG1
Purification Ascites
Antigen
Alternative Name CEBPA (CEBPA Antibody Abstract)
Background The protein encoded by this intronless gene is a bZIP transcription factor which can bind as a homodimer to certain promoters and enhancers. It can also form heterodimers with the related proteins CEBP-beta and CEBP-gamma. The encoded protein has been shown to bind to the promoter and modulate the expression of the gene encoding leptin, a protein that plays an important role in body weight homeostasis. Also, the encoded protein can interact with CDK2 and CDK4, thereby inhibiting these kinases and causing growth arrest in cultured cells.
Molecular Weight 42 kDa
Gene ID 1050
HGNC 1050
Pathways Brown Fat Cell Differentiation, Positive Regulation of fat Cell Differentiation
Application Notes ELISA: 1:10000, WB: 1:500 - 1:2000, IHC: 1:200 - 1:1000, ICC: 1:200 - 1:1000, FCM: 1:200 - 1:400
Restrictions For Research Use only
Format Liquid
Buffer Ascitic fluid containing 0.03 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment 4°C, -20°C for long term storage
Supplier Images
Image no. 1 for anti-CCAAT/enhancer Binding Protein (C/EBP), alpha (CEBPA) antibody (ABIN969048) Figure 2: Flow cytometric analysis of MCF-7 cells using CEBPA mouse mAb (green) and n...
Image no. 2 for anti-CCAAT/enhancer Binding Protein (C/EBP), alpha (CEBPA) antibody (ABIN969048) Figure 3: Immunofluorescence analysis of HeLa cells. Blue: DRAQ5 fluorescent DNA dye....
Image no. 3 for anti-CCAAT/enhancer Binding Protein (C/EBP), alpha (CEBPA) antibody (ABIN969048) Figure 4: Immunofluorescence analysis of HeLa cells using CEBPA mouse mAb (green) . B...
Image no. 4 for anti-CCAAT/enhancer Binding Protein (C/EBP), alpha (CEBPA) antibody (ABIN969048) Figure 5: Immunohistochemical analysis of paraffin-embedded rectum tissues using CEBP...
Image no. 5 for anti-CCAAT/enhancer Binding Protein (C/EBP), alpha (CEBPA) antibody (ABIN969048) Figure 6: Western blot analysis using CEBPA mouse mAb against Jurkat (1), k562 (2), a...
Image no. 6 for anti-CCAAT/enhancer Binding Protein (C/EBP), alpha (CEBPA) antibody (ABIN969048) Figure 1: Red: Control Antigen (100 ng), Purple: Antigen (10ng), Green: Antigen (50 n...
Product cited in: Jin, Zhang, Yan, Liu, Wang, Ge, Zhai: "C/EBPalpha regulates SIRT1 expression during adipogenesis." in: Cell research, Vol. 20, Issue 4, pp. 470-9, 2010 (PubMed).