MAP2K7 antibody

Catalog No. ABIN969271

This Mouse Monoclonal antibody specifically detects MAP2K7 in ELISA, IHC, ICC and FACS. It exhibits reactivity toward Human and has been mentioned in 2+ publications.

Quick Overview for MAP2K7 antibody (ABIN969271)

Target: MAP2K7 (Mitogen-Activated Protein Kinase Kinase 7 (MAP2K7))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This MAP2K7 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Clone: 4E5

Product Details anti-MAP2K7 Antibody

Purpose: MAP2K7 Antibody

Purification: Ascitic fluid

Immunogen: Purified recombinant fragment of human MAP2K7 expressed in E. Coli.

Isotype: IgG1

Application Details

Application Notes:

ELISA: 1/10000

FCM: 1/200 - 1/400

ICC: 1/200 - 1/1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Ascitic fluid containing 0.03 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

References for anti-MAP2K7 antibody (ABIN969271)

Nakagawa, Sugahara, Yamasaki, Kajiho, Takahashi, Hirayama, Minami, Ohta, Watanabe, Hata, Katada, Nishina: "Filamin associates with stress signalling kinases MKK7 and MKK4 and regulates JNK activation." in: The Biochemical journal, Vol. 427, Issue 2, pp. 237-45, (2010) (PubMed).

Yoshizawa, Hammaker, Sweeney, Boyle, Firestein: "Synoviocyte innate immune responses: I. Differential regulation of interferon responses and the JNK pathway by MAPK kinases." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 181, Issue 5, pp. 3252-8, (2008) (PubMed).

Target Details for MAP2K7

Target: MAP2K7 (Mitogen-Activated Protein Kinase Kinase 7 (MAP2K7))

Alternative Name: MAP2K7

Background: The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase specifically activates MAPK8/JNK1 and MAPK9/JNK2, and this kinase itself is phosphorylated and activated by MAP kinase kinase kinases including MAP3K1/MEKK1, MAP3K2/MEKK2,MAP3K3/MEKK5, and MAP4K2/GCK. This kinase is involved in the signal transduction mediating the cell responses to proinflammatory cytokines, and environmental stresses. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found, but only one transcript variant has been supported and defined.

Molecular Weight: 48 kDa

UniProt: O14733

Pathways: MAPK Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades, BCR Signaling