NPT antibody

Catalog No. ABIN969318

This anti- antibody is a Mouse Monoclonal antibody detecting in ELISA. Suitable for Human. This Primary Antibody has been cited in 2+ publications.

Quick Overview for NPT antibody (ABIN969318)

Target: NPT

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: ELISA

Clone: 4B4D1

Product Details

Purpose: NPT Antibody

Purification: Ascitic fluid

Immunogen: Purified recombinant fragment of NPT expressed in E. Coli.

Isotype: IgG2a

Application Details

Application Notes: ELISA: 1/10000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Ascitic fluid containing 0.03 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

References

Lukasik, Bradley, Scott, Dea, Koo, Hsu, Bartz, Farrah: "Reduction of poliovirus 1, bacteriophages, Salmonella montevideo, and Escherichia coli O157:H7 on strawberries by physical and disinfectant washes." in: Journal of food protection, Vol. 66, Issue 2, pp. 188-93, (2003) (PubMed).

Stewart, Richards, Halfhill: "Transgenic plants and biosafety: science, misconceptions and public perceptions." in: BioTechniques, Vol. 29, Issue 4, pp. 832-6, 838-43, (2001) (PubMed).

Target Details

Target: NPT

Background: Neomycin phosphotransferase II (nptII) gene is used in selection of transformed organisms. It was initially isolated from the transposon Tn5 that was present in the bacterium strain Escherichia coli K12. The gene codes for the aminoglycoside 3'-phosphotransferase (denoted aph(3')-II or NPTII) enzyme. NPTII is probably the most widely used selectable marker for plant transformation. It is also used in gene expression and regulation studies in different organisms in part because N-terminal fusions can be constructed that retain enzymatic activity. In animal cells, G418 and neomycin are used as selectable agents.