SMAD5 antibody

Catalog No. ABIN969404

The Mouse Monoclonal anti-SMAD5 antibody has been validated for WB, ELISA, IHC, ICC and FACS. It is suitable to detect SMAD5 in samples from Human and Rat. There are 2+ publications available.

Quick Overview for SMAD5 antibody (ABIN969404)

Target: SMAD5 (SMAD, Mothers Against DPP Homolog 5 (SMAD5))

Reactivity: Human, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This SMAD5 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Clone: 3H9

Product Details anti-SMAD5 Antibody

Purpose: SMAD5 Antibody

Purification: Ascitic fluid

Immunogen: Purified recombinant fragment of human SMAD5 expressed in E. Coli.

Isotype: IgG1

Application Details

Application Notes:

ELISA: 1/10000

FCM: 1/200 - 1/400

ICC: 1/200 - 1/1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Ascitic fluid containing 0.03 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

References for anti-SMAD5 antibody (ABIN969404)

Chang, Chang, Lee, Lee, Yeh, Yeh, Cheng, Chien, Chiu: "Tumor cell cycle arrest induced by shear stress: Roles of integrins and Smad." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, Issue 10, pp. 3927-32, (2008) (PubMed).

Monzen, Ito, Naito, Kasai, Hiroi, Hayashi, Shiojima, Yamazaki, Miyazono, Asashima, Nagai, Komuro: "A crucial role of a high mobility group protein HMGA2 in cardiogenesis." in: Nature cell biology, Vol. 10, Issue 5, pp. 567-74, (2008) (PubMed).

Target Details for SMAD5

Target: SMAD5 (SMAD, Mothers Against DPP Homolog 5 (SMAD5))

Alternative Name: SMAD5

Background: Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD5 is a receptor-regulated SMAD (R-SMAD). SMAD5 is required for normal development of the cardiovascular system in vivo, lack of the SMAD5 gene results in apoptosis of cardiac myocytes. 3 Upregulation of SMAD5 has been reported to mediate apoptosis of gastric epithelial cells induced by Helicobacter pylori infection. Tissue specificity: Ubiquitous.

Molecular Weight: 52 kDa

UniProt: Q99717