Vimentin antibody (AA 2-466)

Catalog No. ABIN969454

This Mouse Monoclonal antibody specifically detects Vimentin in WB, IHC and ELISA. It exhibits reactivity toward Human and Monkey and has been mentioned in 2+ publications.

Quick Overview for Vimentin antibody (AA 2-466) (ABIN969454)

Target: Vimentin (VIM)

Reactivity: Human, Monkey

Host: Mouse

Clonality: Monoclonal

Conjugate: This Vimentin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Clone: 4F2E9

Product Details anti-Vimentin Antibody

Binding Specificity: AA 2-466

Purpose: Vimentin Antibody

Purification: Ascitic fluid

Immunogen: Purified recombinant fragment of Vimentin (aa2-466) expressed in E. Coli.

Isotype: IgG1

Application Details

Application Notes: ELISA: 1/10000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Ascitic fluid containing 0.03 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

References for anti-Vimentin antibody (ABIN969454)

Yates, Zetterberg, Rajeev, Reiss, Rittling: "Promoter-independent regulation of vimentin expression in mammary epithelial cells by val(12)ras and TGFbeta." in: Experimental cell research, Vol. 313, Issue 17, pp. 3718-28, (2007) (PubMed).

Gilles, Polette, Mestdagt, Nawrocki-Raby, Ruggeri, Birembaut, Foidart: "Transactivation of vimentin by beta-catenin in human breast cancer cells." in: Cancer research, Vol. 63, Issue 10, pp. 2658-64, (2003) (PubMed).

Target Details for Vimentin

Target: Vimentin (VIM)

Alternative Name: Vimentin

Background: Vimentin, also know as VIM. It is the major subunit protein of the intermediate filaments of mesenchymal cells. It is believed to be involved with the intracellular transport of proteins between the nucleus and plasma membrane. Vimentin has been implicated to be involved in the rate of steroid synthesis via its role as a storage network for steroidogenic cholesterol containing lipid droplets. Vimentin phosphorylation by a protein kinase causes the breakdown of intermediate filaments and activation of an ATP and myosin light chain dependent contractile event. This results in cytoskeletal changes that facilitate the interaction of the lipid droplets within mitochondria, and subsequent transport of cholesterol to the organelles leading to an increase in steroid synthesis. Immunohistochemical staining for Vimentin is characteristic of sarcomas (of neural, muscle and fibroblast origin) compared to carcinomas which are generally negative. Melanomas, lymphomas and vascular tumors may all stain for Vimentin. Vimentin antibodies are thus of value in the differential diagnosis of undifferentiated neoplasms and malignant tumors. They are generally used with a panel of other antibodies including those recognising cytokeratins, lymphoid markers, S100, desmin and neurofilaments.

Molecular Weight: 54 kDa

Gene ID: 7431

UniProt: P08670

Pathways: Caspase Cascade in Apoptosis