Glypican 3 antibody

Catalog No. ABIN969523

This Mouse Monoclonal antibody specifically detects Glypican 3 in FACS, WB, IHC, ELISA and ICC. It exhibits reactivity toward Human, Rat and Mouse and has been mentioned in 2+ publications.

Quick Overview for Glypican 3 antibody (ABIN969523)

Target: Glypican 3 (GPC3)

Reactivity: Human, Rat, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This Glypican 3 antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunocytochemistry (ICC)

Clone: 9C2

Product Details anti-Glypican 3 Antibody

Purpose: GPC3 Antibody

Purification: Purified antibody

Immunogen: Purified recombinant fragment of human GPC3 expressed in E. Coli.

Isotype: IgG1

Application Details

Application Notes:

ELISA: 1/10000

FCM: 1/200 - 1/400

ICC: 1/200 - 1/1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified antibody in PBS with 0.05 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

References for anti-Glypican 3 antibody (ABIN969523)

Zittermann, Capurro, Shi, Filmus: "Soluble glypican 3 inhibits the growth of hepatocellular carcinoma in vitro and in vivo." in: International journal of cancer. Journal international du cancer, Vol. 126, Issue 6, pp. 1291-301, (2010) (PubMed).

Takai, Ashihara, Ishiguro, Terashima, Watanabe, Kato, Suzuki: "Involvement of glypican-3 in the recruitment of M2-polarized tumor-associated macrophages in hepatocellular carcinoma." in: Cancer biology & therapy, Vol. 8, Issue 24, pp. 2329-38, (2010) (PubMed).

Target Details for Glypican 3

Target: Glypican 3 (GPC3)

Alternative Name: GPC3

Background: Cell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains. Members of the glypican-related integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation. The protein encoded by this gene can bind to and inhibit the dipeptidyl peptidase activity of CD26, and it can induce apoptosis in certain cell types. Deletion mutations in this gene are associated with Simpson-Golabi-Behmel syndrome, also known as Simpson dysmorphia syndrome. Alternative splicing results in multiple transcript variants.

Molecular Weight: 65.5 kDa

Gene ID: 2719

UniProt: P51654

Pathways: Glycosaminoglycan Metabolic Process