NEDD8 antibody
(1 reference)Quick Overview for NEDD8 antibody (ABIN969546)
Target
See all NEDD8 AntibodiesReactivity
Host
Clonality
Conjugate
Application
Clone
-
-
Purpose
- NEDD8 Antibody
-
Purification
- Purified antibody
-
Immunogen
- Purified recombinant fragment of human NEDD8 expressed in E. Coli.
-
Isotype
- IgG1
-
-
anti-Neural Precursor Cell Expressed, Developmentally Down-Regulated 8 (NEDD8) antibody
NEDD8 Reactivity: Human WB, IHC, ELISA, FACS, ICC Host: Mouse Monoclonal 5B8 unconjugated
anti-Neural Precursor Cell Expressed, Developmentally Down-Regulated 8 (NEDD8) antibodyNEDD8 Reactivity: Human, Mouse, Rat, Monkey WB, IHC, ELISA, IF, ICC Host: Rabbit Polyclonal unconjugated
anti-Neural Precursor Cell Expressed, Developmentally Down-Regulated 8 (NEDD8) (N-Term) antibodyNEDD8 Reactivity: Human, Mouse, Rat, Monkey, Cow, Xenopus laevis WB, IHC, IF, IHC (p), FACS, IP Host: Rabbit Polyclonal unconjugated
anti-Neural Precursor Cell Expressed, Developmentally Down-Regulated 8 (NEDD8) antibodyNEDD8 Reactivity: Human WB, IF, IHC (p), FACS, EIA Host: Mouse Monoclonal 1A7 unconjugated
anti-Neural Precursor Cell Expressed, Developmentally Down-Regulated 8 (NEDD8) (AA 1-81) antibodyNEDD8 Reactivity: Human WB, IHC, ELISA, IF Host: Rabbit Polyclonal unconjugated
anti-Neural Precursor Cell Expressed, Developmentally Down-Regulated 8 (NEDD8) (N-Term) antibodyNEDD8 Reactivity: Human, Mouse, Rat, Monkey WB, IHC, ELISA Host: Rabbit Polyclonal unconjugated
anti-Neural Precursor Cell Expressed, Developmentally Down-Regulated 8 (NEDD8) (AA 1-32), (N-Term) antibodyNEDD8 Reactivity: Human WB, IHC (p) Host: Rabbit Polyclonal RB0639 unconjugated
anti-Neural Precursor Cell Expressed, Developmentally Down-Regulated 8 (NEDD8) (AA 1-32) antibodyNEDD8 Reactivity: Human WB, IHC, ELISA Host: Rabbit Polyclonal unconjugated
anti-Neural Precursor Cell Expressed, Developmentally Down-Regulated 8 (NEDD8) (N-Term) antibodyNEDD8 Reactivity: Human WB, IHC, ELISA Host: Rabbit Polyclonal unconjugated
anti-Neural Precursor Cell Expressed, Developmentally Down-Regulated 8 (NEDD8) antibodyNEDD8 Reactivity: Human WB, IHC, ELISA, IF, IHC (p), FACS Host: Mouse Monoclonal 5B8 unconjugated
-
-
Application Notes
-
ELISA: 1/10000
FCM: 1/200 - 1/400
ICC: 1/200 - 1/1000
-
Restrictions
- For Research Use only
-
-
-
Format
- Liquid
-
Buffer
- Purified antibody in PBS with 0.05 % sodium azide.
-
Preservative
- Sodium azide
-
Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
Storage
- 4 °C,-20 °C
-
Storage Comment
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
-
-
-
: "The mechanism of poly-NEDD8 chain formation in vitro." in: Biochemical and biophysical research communications, Vol. 381, Issue 3, pp. 443-7, (2009) (PubMed).
-
: "The mechanism of poly-NEDD8 chain formation in vitro." in: Biochemical and biophysical research communications, Vol. 381, Issue 3, pp. 443-7, (2009) (PubMed).
-
- NEDD8 (Neural Precursor Cell Expressed, Developmentally Down-Regulated 8 (NEDD8))
-
Alternative Name
- NEDD8
-
Background
- Ubiquitin-like protein which plays an important role in cell cycle control and embryogenesis. Covalent attachment to its substrates requires prior activation by the E1 complex UBE1C-APPBP1 and linkage to the E2 enzyme UBE2M. Attachment of NEDD8 to cullins activates their associated E3 ubiquitin ligase activity, and thus promotes polyubiquitination and proteasomal degradation of cyclins and other regulatory proteins.Tissue specificity: Highly expressed in heart, skeletal muscle, spleen, thymus, prostate, testis, ovary, colon and leukocytes.
-
Molecular Weight
- 9 kDa
-
Gene ID
- 4738
-
UniProt
- Q15843
-
Pathways
- Ubiquitin Proteasome Pathway
Target
-
