ROCK1 antibody

Catalog No. ABIN969563

There are 2+ publications for this product available. The Mouse Monoclonal anti-ROCK1 antibody is suitable to detect ROCK1 in samples from Human. It has been validated for ELISA.

Quick Overview for ROCK1 antibody (ABIN969563)

Target: ROCK1 (rho-Associated, Coiled-Coil Containing Protein Kinase 1 (ROCK1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This ROCK1 antibody is un-conjugated

Application: ELISA

Clone: 1H4

Product Details anti-ROCK1 Antibody

Purpose: ROCK1 Antibody

Purification: Purified antibody

Immunogen: Purified recombinant fragment of human ROCK1 expressed in E. Coli.

Isotype: IgG1

Application Details

Application Notes: ELISA: 1/10000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified antibody in PBS with 0.05 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

References for anti-ROCK1 antibody (ABIN969563)

Kroll, Epting, Kern, Dietz, Feng, Hammes, Wieland, Augustin: "Inhibition of Rho-dependent kinases ROCK I/II activates VEGF-driven retinal neovascularization and sprouting angiogenesis." in: American journal of physiology. Heart and circulatory physiology, Vol. 296, Issue 3, pp. H893-9, (2009) (PubMed).

Narumiya, Tanji, Ishizaki: "Rho signaling, ROCK and mDia1, in transformation, metastasis and invasion." in: Cancer metastasis reviews, Vol. 28, Issue 1-2, pp. 65-76, (2009) (PubMed).

Target Details for ROCK1

Target: ROCK1 (rho-Associated, Coiled-Coil Containing Protein Kinase 1 (ROCK1))

Alternative Name: ROCK1

Background: This gene encodes a protein serine/threonine kinase that is activated when bound to the GTP-bound form of Rho. The small GTPase Rho regulates formation of focal adhesions and stress fibers of fibroblasts, as well as adhesion and aggregation of platelets and lymphocytes by shuttling between the inactive GDP-bound form and the active GTP-bound form. Rho is also essential in cytokinesis and plays a role in transcriptional activation by serum response factor. This protein, a downstream effector of Rho, phosphorylates and activates LIM kinase, which in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity. ,

Molecular Weight: 158 kDa

UniProt: Q13464

Pathways: Microtubule Dynamics, WNT Signaling, M Phase, Maintenance of Protein Location, Signaling Events mediated by VEGFR1 and VEGFR2, Thromboxane A2 Receptor Signaling