Cyclin B1 antibody (CCNB1)

Details for Product anti-CCNB1 Antibody No. ABIN97948
Antigen
Reactivity
Human
439
230
148
45
7
7
7
6
4
4
3
2
1
1
1
Host
Rabbit
321
132
1
Clonality
Polyclonal
Conjugate
This Cyclin B1 antibody is un-conjugated
18
11
11
11
11
11
11
9
9
5
4
4
4
4
4
4
4
2
2
2
2
2
2
2
2
2
2
2
2
2
1
Application
Immunoprecipitation (IP), ELISA, Western Blotting (WB)
255
125
118
110
90
71
56
52
20
19
16
16
14
8
7
4
3
3
2
1
Options
Immunogen Anti-Cyclin B1 antibody was produced by repeated immunizations of full length fusion protein corresponding to the human gene.
Immunogentype:Native
Characteristics Concentration Definition: by Refractometry
Purification This product was prepared from monospecific antiserum by delipidation and defibrination.
Antigen
Alternative Name Cyclin B1 (CCNB1 Antibody Abstract)
Background The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product forms a complex with p34 (cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites.
Synonyms: G2/mitotic-specific cyclin-B1
Gene ID 891, 14327896
UniProt P14635
Pathways Cell Division Cycle, AMPK Signaling, Mitotic G1-G1/S Phases, M Phase
Application Notes This antibody is suitable for use in ELISA, immunoblotting, immunoprecipitation and other immuno-logical methods requiring high titer and specificity.   Specific conditions for reactivity and detection of Cyclin B1 should be optimized by the end user.  Expect a band approximately 55-66 kDa in size corresponding to Cyclin B1 by western blotting in the appropriate cell lysate or extract. H23 cells may be used as a positive control.
Restrictions For Research Use only
Format Liquid
Concentration 80 mg/mL
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Supplier Images
Image no. 1 for anti-Cyclin B1 (CCNB1) antibody (ABIN97948) Western blot analysis using Anti-Cyclin B1 antibody shows detection of human Cyclin ...
Image no. 2 for anti-Cyclin B1 (CCNB1) antibody (ABIN97948) Western blot analysis using Anti-Cyclin B1 antibody shows detection of Cyclin B1 pre...
Image no. 3 for anti-Cyclin B1 (CCNB1) antibody (ABIN97948) anti-Cyclin B1 antibody was diluted 1:500 to detect Cyclin B1 in human brain cerebell...
Product cited in: Wetzel-Strong, Li, Klein, Nishikimi, Caron: "Epicardial-derived adrenomedullin drives cardiac hyperplasia during embryogenesis." in: Developmental dynamics : an official publication of the American Association of Anatomists, Vol. 243, Issue 2, pp. 243-56, 2014 (PubMed).