FOLR1 antibody (Biotin)

Catalog No. ABIN99372

Product Details anti-FOLR1 Antibody (Biotin)

Target: FOLR1 (Folate Receptor 1 (Adult) (FOLR1))

Reactivity: Cow

Host: Goat

Clonality: Polyclonal

Conjugate: This FOLR1 antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Cross-Reactivity (Details): Cross reactivity against Folate Binding Protein from other sources is unknown.

Purity: Anti-Folate Binding Protein is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum as well as purified and partially purified Folate Binding Protein [Bovine Milk].

Endotoxin Level: Low Endotoxin : No

Immunogen: Folate Binding Protein [Bovine Milk]
Immunogen Type: Native Protein

Isotype: IgG

Application Details

Application Notes: Anti-Folate Binding Protein has been assayed against 1.0 µg of Folate Binding Protein in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:4.000 to 1:20.000 of the reconstitution concentration is suggested for this product. This product has been assayed against 1.0 µg of Folate Binding Protein in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:4.000 to 1:20.000 of the reconstitution concentration is suggested for this product.
ELISA Dilution: 1:5.000 - 1:20.000
IF Immunoprecipitation Dilution: 1:100
Western Blot Dilution: 1:500 - 1:5.000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 10 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Handling Advice: Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Storage: -20 °C

Storage Comment: Store vial at -20 °C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.

Expiry Date: Expiration date is one (1) year from date of opening.

References for anti-FOLR1 antibody (Biotin) (ABIN99372)

Kameyama, Motoyama, Tanaka, Yamashita, Higashi, Arima: "Induction of mitophagy-mediated antitumor activity with folate-appended methyl-β-cyclodextrin." in: International journal of nanomedicine, Vol. 12, pp. 3433-3446, (2017) (PubMed).

Target Details for FOLR1

Target: FOLR1 (Folate Receptor 1 (Adult) (FOLR1))

Alternative Name: Folate Binding Protein (FOLR1 Products)

Background: Anti-Folate Binding Protein antibody belongs to the folate receptor family and binds to folate and reduced folic acid derivatives. It further regulates delivery of 5-methyltetrahydrofolate to the cell interior. Folate expresses solely in epithelial tissues, and expression is increased in malignant tissues. Anti-Folate Binding Protein antibody is ideal for researchers investigating Tags & Cell Markers and Cancer research. Synonyms: Folate receptor alpha FR-alpha Folate receptor 1 Milk folate-binding protein Folate-binding protein 1 FBP

Gene ID: 539750

UniProt: P02702

Pathways: Dicarboxylic Acid Transport