GST antibody

Catalog No. ABIN99861

Supplier Product No.: 100-101-200

Product Details anti-GST Antibody

Target: GST (Glutathione S Transferase (GST))

Reactivity: Schistosoma japonicum

Host: Goat

Clonality: Polyclonal

Conjugate: This GST antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Supplier Product No.: 100-101-200

Supplier: Rockland

Purpose: GST Antibody

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-goat serum, purified and partially purified Glutathione-S-Transferase [Schistosoma japonicum].

Characteristics: Synonyms: goat anti-GST Antibody, GST, Glutathione-S-Transferase

Purification: This product was prepared from monospecific antiserum by a delipidation and defibrination.

Immunogen:

Immunogen: Glutathione-S-Transferase [Schistosoma japonicum]

Immunogen Type: Native Protein

Application Details

Application Notes:

Immunohistochemistry Dilution: User Optimized

Application Note: Anti-GST has been tested by western blot and is suitable to be assayed against 1.0 μg of Glutathione-S-Transferase [Schistosoma japonicum] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Goat IgG [H&L] (Rabbit) code #605-4302 and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:1,500 to 1:8,000 of the reconstitution concentration is suggested for this product.

Western Blot Dilution: 1:1,000 - 1:5,000

ELISA Dilution: 1:10,000 - 1:50,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 2.0 mL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 90 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Expiry Date: 12 months

References for anti-GST antibody (ABIN99861)

Miller, Tejada, Gazzano-Santoro: "Development of an ELISA based bridging assay as a surrogate measure of ADCC." in: Journal of immunological methods, (2012) (PubMed).

Target Details for GST

Target: GST (Glutathione S Transferase (GST))

Alternative Name: GST (GST Products)

Background: Background: Rockland produces a wide range of GST antibodies in our laboratories. Select GST antibodies from several monoclonal and/or polyclonal GST antibodies listed below. Select appropriate GST antibodies for your research by isotype, epitope, applications and species reactivity. GST (Glutathione-S-Transferase) is a protein expression tag commonly used in molecular biology. Anti-GST will react with synthetic construct present in most known GST containing cloning or expression vectors. GST is responsible for the conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. The amino acid sequence GST is highly conserved in most organisms including mammals. GST exists as a 26 kDa homodimer.