GST antibody
-
- Target See all GST Antibodies
- GST (Glutathione S Transferase (GST))
-
Reactivity
- Schistosoma japonicum
-
Host
- Goat
-
Clonality
- Polyclonal
-
Conjugate
- This GST antibody is un-conjugated
-
Application
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
- Supplier Product No.
- 100-101-200
- Supplier
- Rockland
- Purpose
- GST Antibody
- Cross-Reactivity (Details)
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-goat serum, purified and partially purified Glutathione-S-Transferase [Schistosoma japonicum].
- Characteristics
- Synonyms: goat anti-GST Antibody, GST, Glutathione-S-Transferase
- Purification
- This product was prepared from monospecific antiserum by a delipidation and defibrination.
- Immunogen
-
Immunogen: Glutathione-S-Transferase [Schistosoma japonicum]
Immunogen Type: Native Protein
- Top Product
- Discover our top product GST Primary Antibody
-
-
- Application Notes
-
Immunohistochemistry Dilution: User Optimized
Application Note: Anti-GST has been tested by western blot and is suitable to be assayed against 1.0 μg of Glutathione-S-Transferase [Schistosoma japonicum] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Goat IgG [H&L] (Rabbit) code #605-4302 and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:1,500 to 1:8,000 of the reconstitution concentration is suggested for this product.
Western Blot Dilution: 1:1,000 - 1:5,000
ELISA Dilution: 1:10,000 - 1:50,000
Other: User Optimized
- Restrictions
- For Research Use only
-
- Format
- Lyophilized
- Reconstitution
-
Reconstitution Volume: 2.0 mL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- Concentration
- 90 mg/mL
- Buffer
-
Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide - Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- Expiry Date
- 12 months
-
-
Development of an ELISA based bridging assay as a surrogate measure of ADCC." in: Journal of immunological methods, (2012) (PubMed).
: "
-
Development of an ELISA based bridging assay as a surrogate measure of ADCC." in: Journal of immunological methods, (2012) (PubMed).
-
- Target
- GST (Glutathione S Transferase (GST))
- Alternative Name
- GST (GST Products)
- Background
- Background: Rockland produces a wide range of GST antibodies in our laboratories. Select GST antibodies from several monoclonal and/or polyclonal GST antibodies listed below. Select appropriate GST antibodies for your research by isotype, epitope, applications and species reactivity. GST (Glutathione-S-Transferase) is a protein expression tag commonly used in molecular biology. Anti-GST will react with synthetic construct present in most known GST containing cloning or expression vectors. GST is responsible for the conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. The amino acid sequence GST is highly conserved in most organisms including mammals. GST exists as a 26 kDa homodimer.
-