GSK3 alpha antibody (pSer21)

Catalog No. ABIN99927

Supplier Product No.: 600-401-429

Product Details anti-GSK3 alpha Antibody

Target: GSK3 alpha (GSK3a) (Glycogen Synthase Kinase 3 alpha (GSK3a))

Binding Specificity: pSer21

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GSK3 alpha antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Fluorescence Microscopy (FM)

Supplier Product No.: 600-401-429

Supplier: Rockland

Purpose: GSK3 Alpha phospho S21 Antibody

Cross-Reactivity (Details): This affinity-purified antibody is directed against the phosphorylated form of human GSK3A at the pS 21 residue.

Characteristics: Synonyms: rabbit anti-GSK3 alpha pS21 antibody, rabbit anti-GSK3α pS21 antibody, GSK 3 alpha pS21 antibody, Glycogen synthase kinase-3 alpha antibody, phosphorylated GSK-3 alpha antibody, GSK 3A antibody, GSK-3A antibody, GSK3alpha antibody, Serine/threonine-protein kinase GSK3A

Purification: The product was affinity purified from monospecific antiserum by immunoaffinity purification.

Sterility: Sterile filtered

Immunogen:

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the N-Terminal region near aa 1-25 of human GSK3 alpha.

Immunogen Type: Conjugated Peptide

Isotype: IgG

Application Details

Application Notes:

Application Note: This affinity-purified antibody has been tested for use in ELISA and by western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 52 kDa in size corresponding to GSK3A by western blotting in the appropriate cell lysate or extract.  Less than 1 % reactivity is observed against the non-phosphorylated form of the immunizing peptide.  This antibody is phospho specific for pS21 of GSK3A.

Western Blot Dilution: 1:500 - 1:3,000

ELISA Dilution: 1:2,000 - 1:10,000

IF Microscopy Dilution: User Optimized

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.28 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for GSK3 alpha

Target: GSK3 alpha (GSK3a) (Glycogen Synthase Kinase 3 alpha (GSK3a))

Alternative Name: GSK3A (GSK3a Products)

Background: Background: Glycogen synthase kinase 3 alpha (GSK3A) belongs to the ser/thr family of protein kinases, Cdc2/cdkx subfamily, gsk-3 subsubfamily. It is implicated in the hormonal control of several regulatory proteins including glycogen synthase, myb, and the transcription factor c-jun.  GSK3A is a proline-directed serine-threonine kinase that was initially identified as a phosphorylating and inactivating glycogen synthase. Two isoforms, alpha (GSK3A) and beta (GSK3B), show a high degree of amino acid homology. GSK3B is involved in energy metabolism, neuronal cell development, and body pattern formation.

Gene ID: 2931, 49574532

UniProt: P49840

Pathways: PI3K-Akt Signaling, WNT Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, cAMP Metabolic Process, Cellular Glucan Metabolic Process, Regulation of Muscle Cell Differentiation, Regulation of G-Protein Coupled Receptor Protein Signaling, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, BCR Signaling, Warburg Effect