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FACS Antibodies

Fluorescence-activated cell sorting (FACS) is a specialized type of flow cytometry. It sorts a heterogeneous mixture of biological cells into two or more containers, one cell at a time, based upon the specific light scattering and fluorescent characteristics of each cell and its attached antibodies.

Important Targets

Arginase 1 Antibodies

CASP8 Antibodies

CCR2 Antibodies

CD14 Antibodies

FOXP3 Antibodies

IL17 Antibodies

Lamin B1 Antibodies

All Flow Cytometry Antibodies

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84,653 results

SOX2 antibody (AA 227-317)

SOX2 Reactivity: Human, Mouse, Rat, Goat WB, ELISA, FACS, ICC, IHC (p), IF (p), IF (cc), IHC (fro) Host: Rabbit Polyclonal unconjugated

Formalin-fixed and paraffin embedded human stomach labeled with Anti-SOX2 Polyclonal Antibody, Unconjugated (ABIN669621) at 1:200 followed by conjugation to the secondary antibody and DAB staining

Lane 1: mouse embryo lysates Lane 2: mouse lung lysates probed with Anti SOX2 Polyclonal Antibody, Unconjugated (ABIN669621) at 1:200 in 4 °C.

Formalin-fixed and paraffin embedded rat esophagus labeled with Anti-SOX2 Polyclonal Antibody, Unconjugated (ABIN669621) at 1:200 followed by conjugation to the secondary antibody and DAB staining

NCCIT cells probed with Rabbit Anti-SOX2 Polyclonal Antibody Alexa Fluor 488 Conjugated Secondary Antibody.

Formalin-fixed and paraffin embedded human stomach labeled with Anti-SOX2 Polyclonal Antibody, Unconjugated at 1:200 followed by conjugation to the secondary antibody and DAB staining

Formalin-fixed and paraffin embedded rat esophagus labeled with Anti-SOX2 Polyclonal Antibody, Unconjugated at 1:200 followed by conjugation to the secondary antibody and DAB staining

6 images

8 references

Catalog No. ABIN669621

Aquaporin 4 antibody (AA 271-323)

AQP4 Reactivity: Human, Mouse, Rat WB, ICC, FACS, IHC (p), IF (p), IF (cc), IHC (fro) Host: Rabbit Polyclonal unconjugated

Rat Lung lysates probed with Anti- AQP4 Polyclonal Antibody, Unconjugated at 1:5000 90min in 37˚C.

Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti Annexin A13 Polyclonal Antibody, Unconjugated (ABIN671181) at 1:200 followed by conjugation to the secondary antibody and DAB staining

Formalin-fixed and paraffin embedded mouse kidney labeled with Rabbit Anti AQP4 Polyclonal Antibody, Unconjugated (ABIN671181) at 1:200 followed by conjugation to the secondary antibody and DAB staining

Formalin-fixed and paraffin embedded rat spinal cord tissue labeled with Anti-AQP4 Polyclonal Antibody, Unconjugated (ABIN671181) at 1:200, followed by conjugation to the secondary antibody and DAB staining

Human A549 cell lysates probed with Rabbit Anti-Aquaporin 4 Polyclonal Antibody, Unconjugated .

Formalin-fixed and paraffin embedded rat spinal cord tissue labeled with Anti-AQP4 Polyclonal Antibody, Unconjugated at 1:200, followed by conjugation to the secondary antibody and DAB staining

6 images

4 references

Catalog No. ABIN671181

CD34 antibody (AA 301-385)

CD34 Reactivity: Human, Rat, Mouse, Rabbit, Chicken, Goat FACS, WB, ELISA, IHC (p), IHC (fro), IF (p), IF (cc) Host: Rabbit Polyclonal unconjugated

Mouse brain lysates probed with Anti-CD34 Polyclonal Antibody, Unconjugated at 1:5000 90min in 37˚C.

Mouse SP20 Myeloma Cells

Formalin-fixed and paraffin embedded human gastric adenocarcinoma labeled Anti-CD34 Polyclonal Antibody, Unconjugated (ABIN671361) at 1:200, followed by conjugation to the secondary antibody Goat Anti-Rabbit IgG, AF488 conjugated used at 1:200 dilution for 40 minutes at 37°C

Antigen: 0.

Mouse lung lysate probed with Anti-CD34 Polyclonal Antibody, Unconjugated at 1:5000 for 90 min at 37˚C.

5 images

21 references

Catalog No. ABIN671361

Survivin antibody (AA 85-142)

BIRC5 Reactivity: Human, Mouse WB, ELISA, IHC (p), FACS, IF (cc), IF (p), IHC (fro) Host: Rabbit Polyclonal unconjugated

Human Gastric Cancer Cells labeled with Anti-Survivin Polyclonal Antibody, Unconjugated (ABIN670911) at 1:300, followed by conjugation to the secondary antibody and DAB staining

Formalin-fixed human oral squamous cell carcinoma labeled with Anti-Survivin Polyclonal Antibody, Cy3 conjugated (ABIN670911) used at 1:200 dilution for 40 minutes at 37°C, followed by DAPI staining

Formalin-fixed and paraffin embedded human colon carcinoma labeled with Anti-Survivin Polyclonal Antibody, Unconjugated (ABIN670911) at 1:200, followed by conjugation to the secondary antibody and DAB staining

Formalin-fixed and paraffin embedded human oral squamous cell carcinoma labeled with Rabbit Anti-Survivin Polyclonal Antibody, Unconjugated at 1:200 followed by Goat Anti-Rabbit IgG, FITC conjugated -FITC) used at 1:300 dilution for 40 minutes at 37°C.

Formalin-fixed and paraffin embedded human colon carcinoma labeled with Anti-Survivin Polyclonal Antibody, Unconjugated at 1:200, followed by conjugation to the secondary antibody and DAB staining

U251 cells probed with Survivin Polyclonal Antibody, Unconjugated for 30 minutes followed by incubation with a conjugated secondary (FITC Conjugated) (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).

Human Gastric Cancer Cells labeled with Anti-Survivin Polyclonal Antibody, Unconjugated at 1:300, followed by conjugation to the secondary antibody and DAB staining

Mouse smail intestine lysates probed with Survivin Polyclonal Antibody, Unconjugated at 1:300 dilution and 4˚C overnight incubation.

8 images

1 reference

Catalog No. ABIN670911

C-JUN antibody (AA 31-331)

JUN Reactivity: Human, Mouse, Rat, Chicken WB, ELISA, IHC (p), FACS, IF (cc), IF (p), IHC (fro) Host: Rabbit Polyclonal unconjugated

Formalin-fixed and paraffin embedded rat brain tissue labeled Anti-C-Jun Polyclonal Antibody (ABIN671691), Unconjugated at 1:200, followed by conjugation to the secondary antibody and DAB staining

Image provided by One World Lab validation program.

Image provided by One World Lab validation program.

HepG2 cells probed with c-Jun Polyclonal Antibody, unconjugated at 1:100 dilution for 30 minutes compared to control cells (blue) and isotype control (orange)

Formalin-fixed and paraffin embedded rat brain tissue labeled Anti-C-Jun Polyclonal Antibody , Unconjugated at 1:200, followed by conjugation to the secondary antibody and DAB staining

Paraformaldehyde-fixed, paraffin embedded rat testis, Antigen retrieval by boiling in sodium citrate buffer (pH6.

6 images

5 references

Catalog No. ABIN671691

HMGB1 antibody (AA 75-170)

HMGB1 Reactivity: Human, Mouse, Rat WB, ELISA, FACS, IHC (p), IF (cc), IF (p), IHC (fro) Host: Rabbit Polyclonal unconjugated

Lane 1: Mouse Macrophages probed with Rabbit Anti-HMGB1 Polyclonal Antibody, Unconjugated at 1:5000 for 90 min at 37˚C.

Formalin-fixed and paraffin embedded human endometrium carcinoma labeled Anti-HMGB1 Polyclonal Antibody, Unconjugated (ABIN671616) at 1:200, followed by conjugation to the secondary antibody and DAB staining

Formalin-fixed and paraffin embedded rat liver tissue labeled Anti-HMGB1 Polyclonal Antibody, Unconjugated (ABIN671616) at 1:400, followed by conjugation to the secondary antibody and DAB staining

Human HepG2 cell lysates probed with Rabbit Anti-HMGB1 Polyclonal Antibody, PE-Cy5 conjugated .

Formalin-fixed and paraffin embedded rat liver tissue labeled Anti-HMGB1 Polyclonal Antibody, Unconjugated at 1:400, followed by conjugation to the secondary antibody and DAB staining

Formalin-fixed and paraffin embedded human endometrium carcinoma labeled Anti-HMGB1 Polyclonal Antibody, Unconjugated at 1:200, followed by conjugation to the secondary antibody and DAB staining

6 images

7 references

Catalog No. ABIN671616

Growth Hormone Receptor antibody (AA 101-200)

GHR Reactivity: Human, Mouse, Rat, Pig, Sheep WB, ELISA, FACS, IF (cc), IF (p), IHC (p), IHC (fro) Host: Rabbit Polyclonal unconjugated

Evaluation of the pig GHR transcript with a 4bp insert in vitro.

Formalin-fixed and paraffin embedded rat liver tissue labeled Anti-GHR Polyclonal Antibody, Unconjugated (ABIN671481) at 1:400, followed by conjugation to the secondary antibody and DAB staining

Human A549 cell lysates probed with Rabbit Anti-GHR Polyclonal Antibody, Unconjugated .

This image was generously provided by Brandon Menzies, PhD, from The University of Melbourne.

Formalin-fixed and paraffin embedded rat liver tissue labeled Anti-GHR Polyclonal Antibody, Unconjugated at 1:400, followed by conjugation to the secondary antibody and DAB staining

Knockout of the pig GHR gene in F2 homozygous pigs (GHR4bp/4bp refers to -/-).

6 images

7 references

Catalog No. ABIN671481

CD63 antibody (Biotin)

CD63 Reactivity: Human FACS, IHC (p), IP, ICC Host: Mouse Monoclonal MEM-259 Biotin

Flow cytometry surface staining pattern of IgE stimulated human peripheral whole blood stained using anti-human CD63 (MEM-259) Biotin antibody (concentration in sample 0,6 μg/mL, Streptavidin APC).

Immunofluorescence staining of human skin fibroblasts with anti-CD63 (MEM-259 ; green) after co-incubation of living cells with human Transferrin - Dyomics 547 (red); cell nuclei stained with DAPI (blue).

Immunofluorescence staining of human skin fibroblasts with anti-CD63 (MEM-259 ; green) after co-incubation of living cells with human Transferrin - Dyomics 547; cell nuclei stained with DAPI (blue).

Immunofluorescence staining of CD63 in human HeLa cell line using anti-CD63

Flow cytometry analysis of peripheral blood lymphocytes from a patient with allergy to bee venom after stimulation with bee venom, stained with anti-human CD63 FITC.

Flow cytometry multicolor surface staining of IgE stimulated peripheral blood mononuclear cells stained using anti-human CD63 (MEM-259) Biotin antibody (concentration in sample 0,6 μg/mL, Streptavidin APC) and anti-human CD203c (NP4D6) PE antibody (20 μL reagent / 100 μL of peripheral whole blood).

Separation of CD63 positive CD203c positive basophil granulocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of IgE stimulated human peripheral whole blood using anti-human CD63 (MEM-259) Biotin antibody (concentration in sample 0,6 μg/mL, Streptavidin APC).

7 images

3 references

Catalog No. ABIN1027697

GSTP1 antibody

GSTP1 Reactivity: Human WB, IHC, ELISA, ICC, FACS Host: Mouse Monoclonal 3F2C2 unconjugated

Western blot analysis using GSTP1 mouse mAb against PC3 cell lysate (1) and human cerebellum tissue lysate (2).

Flow cytometric analysis of K562 cells using GSTP1 mouse mAb (green) and negative control (purple).

Immunohistochemical analysis of paraffin-embedded human prostate tissues using GSTP1 mouse mAb with DAB staining.

Western blot analysis using GSTP1 mouse mAb against HEK293T cells transfected with the pCMV6-ENTRY control (1) and pCMV6-ENTRY GSTP1 cDNA (2).

Confocal immunofluorescence analysis of PC-3 cells using GSTP1 mouse mAb (green).

Confocal immunofluorescence analysis of HepG2 (left) and L-02 (right) cells using GSTP1 mouse mAb (green).

6 images

2 references

Catalog No. ABIN1724656

CD44 antibody

CD44 Reactivity: Human, Mouse FACS, IHC, ELISA, ICC Host: Mouse Monoclonal 8E2F3 unconjugated

Western blot analysis using CD44 mouse mAb against truncated Trx-CD44 recombinant protein (1) and GST-CD44 (aa628-699) recombinant protein (2).

Confocal immunofluorescence analysis of methanol-fixed A431 (A), Hela (B), PANC-1 (C) and EC (D) cells using CD44 mouse mAb (green), showing membrane localization.

Confocal immunofluorescence analysis of PANC-1 cells using CD44 mouse mAb (green).

Confocal analysis of paraffin-embedded human lung cancer tissues using CD44 mouse mAb (green), showing membrane localization.

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissues, showing membrane localization with DAB staining using CD44 mouse mAb.

Western blot analysis using CD44 mouse mAb against Hela (1) and HUVE-12(2) cell lysate.

Flow cytometric analysis of Hela cells using CD44 mouse mAb (right) and negative control (left).

7 images

5 references

Catalog No. ABIN969026

CD63 antibody (APC)

CD63 Reactivity: Human FACS Host: Mouse Monoclonal MEM-259 APC

Flow cytometry analysis of IgE-activated peripheral blood stained with anti-human CD63 (MEM-259) APC.

Flow cytometry analysis of peripheral blood lymphocytes from a patient with allergy to bee venom after stimulation with bee venom, stained with anti-human CD63 FITC.

Immunofluorescence staining of human skin fibroblasts with anti-CD63 (MEM-259 ; green) after co-incubation of living cells with human Transferrin - Dyomics 547; cell nuclei stained with DAPI (blue).

Immunofluorescence staining of CD63 in human HeLa cell line using anti-CD63

Immunofluorescence staining of human skin fibroblasts with anti-CD63 (MEM-259 ; green) after co-incubation of living cells with human Transferrin - Dyomics 547 (red); cell nuclei stained with DAPI (blue).

Flow cytometry analysis of IgE-activated peripheral blood stained with anti-human CD63 (MEM-259) APC.

6 images

2 references

Catalog No. ABIN192268

MAP1LC3A antibody (AA 1-121)

MAP1LC3A Reactivity: Human ELISA, IHC, FACS Host: Mouse Monoclonal 5B10D8 unconjugated

Western blot analysis using MAP1LC3A mAb against human MAP1LC3A recombinant protein.

Immunohistochemical analysis of paraffin-embedded testis tissues using MAP1LC3A mouse mAb with DAB staining.

Flow cytometric analysis of HeLa cells using MAP1LC3A mouse mAb (green) and negative control (purple).

Western blot analysis using MAP1LC3A mAb against HEK293 (1) and MAP1LC3A (AA: 1-121)-hIgGFc transfected HEK293 (2) cell lysate.

Black line: Control Antigen (100 ng), Purple line: Antigen(10 ng), Blue line: Antigen (50 ng), Red line: Antigen (100 ng),

Immunohistochemical analysis of paraffin-embedded liver cancer tissues using MAP1LC3A mouse mAb with DAB staining.

6 images

2 references

Catalog No. ABIN1724767

NPC1 antibody (AA 34-174)

NPC1 Reactivity: Human ELISA, IHC, FACS Host: Mouse Monoclonal 8D10G3 unconjugated

Western blot analysis using NPC1 mAb against human NPC1 recombinant protein.

Black line: Control Antigen (100 ng), Purple line: Antigen(10 ng), Blue line: Antigen (50 ng), Red line: Antigen (100 ng),

Flow cytometric analysis of Hela cells using NPC1 mouse mAb (green) and negative control (red).

Western blot analysis using NPC1 mAb against HEK293 (1) and NPC1 (AA: 34-174)-hIgGFc transfected HEK293 (2) cell lysate.

Immunohistochemical analysis of paraffin-embedded liver cancer tissues using NPC1 mouse mAb with DAB staining.

Immunohistochemical analysis of paraffin-embedded esophageal cancer tissues using NPC1 mouse mAb with DAB staining.

6 images

2 references

Catalog No. ABIN1724840

SQSTM1 antibody (AA 232-356)

SQSTM1 Reactivity: Human WB, IHC, ELISA, FACS Host: Mouse Monoclonal 5H7E2 unconjugated

Western blot analysis using SQSTM1 mouse mAb against Hela (1), Jurkat (2), THP-1 (3), HEK293 (4), A549 (5), MCF-7 (6), HepG2 (7), COS7 (8) and SK-BR-3 (9) cell lysate.

Black line: Control Antigen (100 ng), Purple line: Antigen(10 ng), Blue line: Antigen (50 ng), Red line: Antigen (100 ng),

Flow cytometric analysis of HEK293 cells using SQSTM1 mouse mAb (green) and negative control (red).

Western blot analysis using SQSTM1 mAb against human SQSTM1 recombinant protein.

Western blot analysis using SQSTM1 mAb against HEK293 (1) and SQSTM1 (AA: 232-356)-hIgGFc transfected HEK293 (2) cell lysate.

Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using SQSTM1 mouse mAb with DAB staining.

Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using SQSTM1 mouse mAb with DAB staining.

7 images

1 reference

Catalog No. ABIN1724836

CTNNB1 antibody (C-Term)

CTNNB1 Reactivity: Human WB, IF, FACS, IHC (p) Host: Rabbit Polyclonal RB41124 unconjugated

CTNNB1 Antibody (C-term) A western blot analysis in 293,T47D cell line lysates (35 μg/lane).

CTNNB1 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

Confocal immunofluorescent analysis of CTNNB1 Antibody (C-term) with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).

Confocal immunofluorescent analysis of CTNNB1 Antibody (C-term) with T47D cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).

CTNNB1 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.

CTNNB1 Antibody (C-term) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).

6 images

5 references

Catalog No. ABIN1881238

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