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FACS Antibodies

Fluorescence-activated cell sorting (FACS) is a specialized type of flow cytometry. It sorts a heterogeneous mixture of biological cells into two or more containers, one cell at a time, based upon the specific light scattering and fluorescent characteristics of each cell and its attached antibodies.

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Arginase 1 Antibodies

CASP8 Antibodies

CCR2 Antibodies

CD14 Antibodies

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IL17 Antibodies

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All Flow Cytometry Antibodies

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83,890 results

MEK1 antibody (AA 2-150)

MAP2K1 Reactivity: Human ELISA, IHC (p), IF (cc), IF (p), FACS, IHC (fro) Host: Rabbit Polyclonal unconjugated

Immunohistochemistry validation image for anti-Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) (AA 2-150) antibody (ABIN686482)

Immunohistochemistry validation image for anti-Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) (AA 2-150) antibody (ABIN686482)

Immunohistochemistry validation image for anti-Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) (AA 2-150) antibody (ABIN686482)

Immunohistochemistry validation image for anti-Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) (AA 2-150) antibody (ABIN686482)

Image provided by the Independent Validation Program (badge number 29760).

Images provided the Independent Validation Program (badge number 029646)Formalin-fixed and paraffin embedded human placenta labeled with Rabbit Anti-MEK1 Polyclonal Antibody (ABIN686482) at 1:250 overnight at room temperature followed by conjugation to secondary antibody.

Paraformaldehyde-fixed, paraffin embedded human colon cancer, Antigen retrieval by boiling in sodium citrate buffer (pH6.

7 images

Independent Validation

1 validation

Catalog No. ABIN686482

Independently Validated

PI3K p85 alpha/gamma antibody (pTyr199, pTyr467)

PI3K p85a/g Reactivity: Human, Mouse, Rat, Rabbit WB, ELISA, FACS, IF (cc), IF (p), IHC (p), IHC (fro) Host: Rabbit Polyclonal unconjugated

Western Blotting validation image for anti-Phosphoinositide 3 Kinase, p85 alpha/gamma (PI3K p85a/g) (pTyr199), (pTyr467) antibody (ABIN744743)

Image provided by Independent Validation Program, badge 29801.

Formalin-fixed and paraffin embedded rat heart labeled with Anti-phospho-PI3K p85(Tyr467) /p55(Tyr199) Polyclonal Antibody, Unconjugated (ABIN744743) at 1:200 followed by conjugation to the secondary antibody and DAB staining.

Formalin-fixed and paraffin-embedded rat heart labeled with Anti-phospho-PI3K p85(Tyr467) /p55(Tyr199) Polyclonal Antibody, Unconjugated (ABIN744743) 1:200, overnight at 4°C, The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated used at 1:200 dilution for 40 minutes at 37°C.

Paraformaldehyde-fixed, paraffin embedded rat brain tissue, Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min, Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (normal goat serum) at 37°C for 20min, Antibody incubation with Rabbit Anti-PI3 kinase p85 alpha + gamma (Tyr467+Tyr199) Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by a conjugated secondary and DAB staining

5 images

3 references

Independent Validation

1 validation

Catalog No. ABIN744743

Independently Validated

CD200R1L antibody (AA 151-250)

CD200R1L Reactivity: Human WB, FACS, IF (cc), IF (p), ELISA, IHC (fro), IHC (p), ICC Host: Rabbit Polyclonal unconjugated

Immunohistochemistry validation image for anti-CD200 Receptor 1-Like (CD200R1L) (AA 151-250) antibody (ABIN1715098)

Immunohistochemistry validation image for anti-CD200 Receptor 1-Like (CD200R1L) (AA 151-250) antibody (ABIN1715098)

U937 cell lysates probed with Rabbit Anti-CD200R2 Polyclonal Antibody, Unconjugated at 1:500 for 90 min at 37˚C.

Paraformaldehyde-fixed, paraffin embedded human kidney, Antigen retrieval by boiling in sodium citrate buffer (pH6.

Staining of Placental villus from 7w gestation karyotype-normal pregnancy with Antibody.

5 images

4 references

Independent Validation

2 validations

Catalog No. ABIN1715098

Independently Validated

SYN1 antibody (AA 362-511)

SYN1 Reactivity: Human, Rat, Mouse, Monkey WB, ELISA, IHC, ICC, FACS Host: Mouse Monoclonal 7H10G6 unconjugated

Multiplex Immunohistochemistry validation image for anti-Synapsin I (SYN1) (AA 362-511) antibody (ABIN5542390)

Multiplex Immunohistochemistry validation image for anti-Synapsin I (SYN1) (AA 362-511) antibody (ABIN5542390)

Western blot analysis using SYN1 mAb against human SYN1 (AA: 362-511) recombinant protein.

Immunohistochemical analysis of paraffin-embedded testis tissues using SYN1 mouse mAb with DAB staining.

Western blot analysis using SYN1 mAb against HEK293 (1) and SYN1 (AA: 362-511)-hIgGFc transfected HEK293 (2) cell lysate.

Western blot analysis using SYN1 mouse mAb against SK-N-SH (1), NIH/3T3 (2), U251 (3), C6 (4), A549 (5), MCF-7 (6), and COS7 (7) cell lysate.

Flow cytometric analysis of Hela cells using SYN1 mouse mAb (green) and negative control (red).

Immunofluorescence analysis of GC-7901 cells.

Immunofluorescence analysis of GC-7901 cells using SYN1 mouse mAb (green).

Immunofluorescence analysis of HepG2 cells.

Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Immunofluorescence analysis of HepG2 cells using SYN1 mouse mAb (green).

12 images

Independent Validation

1 validation

Catalog No. ABIN5542390

Independently Validated

NeuN antibody (AA 1-140)

RBFOX3 Reactivity: Human, Mouse WB, ELISA, FACS, ICC Host: Mouse Monoclonal 1H3B2 unconjugated

Western Blotting validation image for anti-RNA Binding Protein, Fox-1 Homolog 3 (RBFOX3) (AA 1-140) antibody (ABIN5542704)

Immunohistochemistry validation image for anti-RNA Binding Protein, Fox-1 Homolog 3 (RBFOX3) (AA 1-140) antibody (ABIN5542704)

Western blot analysis using RBFOX3 mAb against human RBFOX3 (AA: 1-140) recombinant protein.

Immunofluorescence analysis of Hela cells using RBFOX3 mouse mAb (green).

Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Western blot analysis using RBFOX3 mAb against HEK293 (1) and RBFOX3 (AA: 1-140)-hIgGFc transfected HEK293 (2) cell lysate.

Western blot analysis using RBFOX3 mouse mAb against C2C12 (1), RAW264.

Flow cytometric analysis of Hela cells using RBFOX3 mouse mAb (green) and negative control (red).

8 images

Independent Validation

2 validations

Catalog No. ABIN5542704

Independently Validated

TLR9 antibody (AA 868-1016)

TLR9 Reactivity: Human IHC, ELISA, FACS, ICC Host: Mouse Monoclonal 1B12H2 unconjugated

Immunofluorescence validation image for anti-Toll-Like Receptor 9 (TLR9) (AA 868-1016) antibody (ABIN5542492)

Proximity Ligation Assay validation image for anti-Toll-Like Receptor 9 (TLR9) (AA 868-1016) antibody (ABIN5542492)

Western blot analysis using TLR9 mAb against human TLR9 (AA: 868-1016) recombinant protein.

Flow cytometric analysis of A549 cells using TLR9 mouse mAb (green) and negative control (red).

Immunofluorescence analysis of SK-OV-3 cells using TLR9 mouse mAb (green).

Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using TLR9 mouse mAb with DAB staining.

Immunohistochemical analysis of paraffin-embedded endometrial cancer tissues using TLR9 mouse mAb with DAB staining.

Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Western blot analysis using TLR9 mAb against HEK293 (1) and TLR9 (AA: 868-1016)-hIgGFc transfected HEK293 (2) cell lysate.

9 images

Independent Validation

2 validations

Catalog No. ABIN5542492

Independently Validated

ATM antibody (pSer1981)

Rockland 200-301-400s ATM Reactivity: Human WB, IHC, ELISA, IF, IP, FACS, FM Host: Mouse Monoclonal 10H11-E12 unconjugated

Anti-ATM Monoclonal Antibody - Western Blot Anti ATM Mab with human derived HEK293 cells treated with doxorubicin using Protein A Purified Mab anti-ATM Protein Kinase pS1981(clone 10H11.

Anti-ATM Monoclonal Antibody - Immunofluorescence Microscopy anti-ATM pS1981 mouse monoclonal antibody (Catalog # 200-301-400) detects ATM phosphorylated on Ser 1981 by Indirect immunofluorescence microscopy.

Anti-ATM Monoclonal Antibody - Immunofluorescence Microscopy Anti ATM antibody showing overlay of anti-ATM pS1981 staining.

Anti-ATM Monoclonal Antibody-Immunohistochemistry.

Anti-ATMpS1981 Flow Cytometry Flow Cytometry of Mouse anti-ATMpS1981 antibody.

5 images

124 references

Catalog No. ABIN6657494

Rockland Original

SARS-Coronavirus Nucleocapsid Protein (SARS-CoV N) antibody

Rockland 200-401-a50 SARS-CoV N Reactivity: SARS Coronavirus (SARS-CoV), SARS Coronavirus-2 (SARS-CoV-2) ELISA, WB, FACS, IHC, FM Host: Rabbit Polyclonal unconjugated

Western Blot of Rabbit Anti-SARS CoV Nucleocapsid (N) Protein Antibody.

Immunofluorescence of SARS-CoV-2 infection in FFPE cell pellets from in vitro cultured human lung cells infected with SARS-CoV-2.

This immunofluorescence microscopy image shows localization of SARS-CoV-2 infection in human liver ductal organoids.

Immunofluorescence of Rabbit Anti-SARS-CoV Nucleocapsid (N) Antibody.

Western blot using anti-SARS CoV Nucleocapsid (N) protein antibody shows detection of a 46-kDa band corresponding to the protein.

Western Blot of Rabbit Anti-SARS-CoV (N) Antibody tested on Caco-2 Cell Lysates before (mock), 24, and 48 hours post infection (hpi).

Immunohistochemistry results showing positive staining in placenta tissue using Anti-SARS-CoV Nucleocapsid (N) Antibody.

Immunohistochemistry of Rabbit Anti-SARS CoV Nucleocapsid Antibody.

ELISA results of Rabbit Anti-SARS-CoV Nucleocapsid (N) Protein.

Immunofluorescence of Rabbit Anti-SARS CoV (N) Protein Antibody.

Immunofluorescence assay using Rabbit Anti-SARS-CoV Nucleocapsid (N) Antibody, showing viral protein detection.

Immunofluorescence assay using Rabbit Anti-SARS-CoV Nucleocapsid (N) Antibody, showing viral protein synthesis.

Immunofluorescence assay using Rabbit Anti-SARS-CoV Nucleocapsid (N) Antibody, showing viral protein detection.

ELISA results of Rabbit Anti-SARS-CoV Nucleocapsid (N) ProteinVariants.

14 images

97 references

Catalog No. ABIN6952544

Rockland Original

COL3A1 antibody

Rockland 600-401-105-0.1 COL3A1 Reactivity: Human, Cow, Pig WB, IHC, ELISA, IP, FACS, DB, FM Host: Rabbit Polyclonal unconjugated

Immunohistochemistry validation image for anti-Collagen, Type III, alpha 1 (COL3A1) antibody (ABIN5596830)

Immunohistochemistry validation image for anti-Collagen, Type III, alpha 1 (COL3A1) antibody (ABIN5596830)

Immunohistochemistry validation image for anti-Collagen, Type III, alpha 1 (COL3A1) antibody (ABIN5596830)

Immunohistochemistry validation image for anti-Collagen, Type III, alpha 1 (COL3A1) antibody (ABIN5596830)

Western Blot of Rabbit Anti-COLLAGEN III Antibody Lane 1: Human Collagen III Load: 100 ng per lane Primary antibody: Collagen III Antibody at 1:1000 o/n at 4°C Secondary antibody: 649 Goat anti-rabbit at 1:20,000 for 30 min at RT Block: ABIN925618 for 30 min at RT Predicted/Observed size: 138 kDa, 138 kDa

anti collagen III antibody (600-401-105 Lot 26016, 1:400, 45 min RT) showed strong staining in FFPE sections of human skin(left, dermis) with moderate to strong red staining and testis (right) where strong staining was observed within connective tissue between seminiferous tubules.

Immunohistochemistry of Rabbit Anti-collagen type III antibody.

(a) Typical electropherogram of α (I) subunits, oligomers β/γ (I), and collagen type I degradation products, released from healthy and burnt skin.

Typical electropherogram of α (III) subunits, oligomers β/γ (III), and collagen type III degradation products, released from healthy and burned skin.

9 images

5 references

Independent Validation

1 validation

Catalog No. ABIN5596830

Independently Validated

Histone 3 antibody (AA 71-136)

H3 Reactivity: Human, Mouse, Rat WB, ELISA, ICC, IHC (p), FACS, IF (cc), IF (p), IHC (fro) Host: Rabbit Polyclonal unconjugated

K562 Cell lysates probed with Histone H3 Polyclonal Antibody, unconjugated at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 60 minutes at 37°C.

Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti Histone H3 Polyclonal Antibody, Unconjugated (ABIN739109) at 1:200 followed by conjugation to the secondary antibody and DAB staining

Mouse splenocytes probed with Rabbit Anti-Histone H3 Polyclonal Antibody, Unconjugated .

Paraformaldehyde-fixed, paraffin embedded human colon cancer, Antigen retrieval by boiling in sodium citrate buffer (pH6.

Formalin-fixed and paraffin-embedded rat brain labeled with Anti-Histone H3 Polyclonal Antibody, Unconjugated (ABIN739109) 1:200, overnight at 4°C, The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated used at 1:200 dilution for 40 minutes at 37°C.

Formalin-fixed and paraffin embedded mouse intestine labeled with Rabbit Anti Histone H3 Polyclonal Antibody, Unconjugated (ABIN739109) at 1:200 followed by conjugation to the secondary antibody and DAB staining

6 images

13 references

Catalog No. ABIN739109

NLRP3 antibody (AA 15-120)

NLRP3 Reactivity: Human, Mouse, Rat, Dog WB, ELISA, IHC (p), FACS, ICC, IHC (fro), IF (p), IF (cc) Host: Rabbit Polyclonal unconjugated

Decreased cAMP exaggerated acute gouty arthritis in P2Y14R-KO rats.

Antigen: 2 µg/100 µL Primary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; Secondary: HRP conjugated Rabbit Anti-Goat IgG at 1: 5000; TMB staining Read the data in Microplate Reader by 450nm.

Formalin-fixed and paraffin embedded mouse embryo labeled with Rabbit Anti Cryopyrin/CIAS1/NALP3 Polyclonal Antibody, Unconjugated (ABIN1386361) at 1:200 followed by conjugation to the secondary antibody and DAB staining

Formalin-fixed and paraffin embedded human lung carcinoma labeled with Rabbit Anti Cryopyrin/CIAS1/NALP3 Polyclonal Antibody, Unconjugated (ABIN1386361) at 1:200 followed by conjugation to the secondary antibody and DAB staining

This image was generously provided by Adib Zendedel, PhD from RWTH Aachen University.

Formalin-fixed and paraffin embedded mouse intestine labeled with Anti-Cryopyrin Polyclonal Antibody, Unconjugated at 1:200 followed by conjugation to the secondary antibody and DAB staining.

HeLa lysates probed with Rabbit Anti-Cryopyrin Polyclonal Antibody, Unconjugated at 1:5000 for 90 min at 37˚C.

Formalin-fixed and paraffin embedded rat kidney labeled with Anti-Cryopyrin Polyclonal Antibody, Unconjugated at 1:200 followed by conjugation to the secondary antibody and DAB staining.

Formalin-fixed and paraffin embedded mouse intestine labeled with Anti-Cryopyrin Polyclonal Antibody, Unconjugated at 1:200 followed by conjugation to the secondary antibody and DAB staining.

EPD inhibited the IMQ-activated nucleotide-binding oligomerization domain (Nod)-like receptor family pyrin domain-containing 3 (NLRP3) inflammasome signaling pathway.

EPD inhibited the IMQ-activated nucleotide-binding oligomerization domain (Nod)-like receptor family pyrin domain-containing 3 (NLRP3) inflammasome signaling pathway.

NLRP3 inflammasome activation was involved in P2Y14R deficiency.

NLRP3 inflammasome activation was involved in P2Y14R deficiency.

Upregulated cAMP suppressed acute gouty arthritis in vivo.

Pyroptosis stress was invalidated once cAMP increased by Forskolin in vitro.

15 images

11 references

Catalog No. ABIN1386361

Cytochrome C antibody (AA 51-105)

CYCS Reactivity: Human, Rat, Mouse, Rabbit, Chicken WB, FACS, ELISA, IHC (p), ICC, IF (p), IHC (fro), IF (cc) Host: Rabbit Polyclonal unconjugated

Mouse heart probed with Anti-Cytochrome C Polyclonal Antibody Unconjugated at 1:5000 for 90 min at 37˚C.

L1 mouse brain lysates L2 mouse lung lysates probed with Anti Cytochrome C Polyclonal Antibody, Unconjugated (ABIN723590) at 1:200 overnight at 4 °C.

Image kindly submitted by an end-user via Funakoshi.

Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti-Cytochrome C Polyclonal Antibody, Unconjugated 1:300 followed by conjugation to the secondary antibody and DAB staining

HepG2 cells probed with Cytochrome C Polyclonal Antibody, unconjugated at 1:100 dilution for 30 minutes compared to control cells (blue) and isotype control (orange).

Elevation of Srx-1 attenuated SI/R-triggered mitochondrial apoptosis pathwayAfter transfection with Ad-Srx-1, cells were then exposure to SI/R.

Srx-1 enhanced cardiomyocyte resistance to SI/R injury by the PI3K/AKT signalling(A) Western blotting analysis for phospho-AKT and AKT in H9c2 cell treated with Ad-Srx-1 upon SI/R.

Analysis of activation of the intrinsic apoptotic pathway.

8 images

19 references

Catalog No. ABIN723590

CD90 antibody (AA 31-120)

THY1 Reactivity: Human, Mouse, Rat, Cow, Dog, Sheep FACS, WB, IHC (fro), IHC (p), IF (cc), IF (p) Host: Rabbit Polyclonal unconjugated

Formalin-fixed and paraffin embedded rat brain tissue labeled with Anti-Thy-1/CD90/ Thy1.

Sheep fat cells stained withAnti-Thy-1 CD90 Thy1.

Sheep fat cells stained withAnti-Thy-1/CD90/Thy1.

Mouse brain lysates probed with CD90 Polyclonal Antibody, Unconjugated at 1:300 dilution and 4˚C overnight incubation.

Formalin-fixed and paraffin embedded rat brain tissue labeled with Anti-Thy-1/CD90/ Thy1.

Mouse splenocytes probed with Rabbit Anti-Thy-1 Polyclonal Antibody, PE-Cy5.

6 images

23 references

Catalog No. ABIN728038

VEGF antibody (AA 27-120)

VEGF Reactivity: Human, Mouse, Rat, Pig, Goat WB, ELISA, IHC (p), IHC (fro), IF (p), FACS Host: Rabbit Polyclonal unconjugated

Formalin-fixed and paraffin embedded: human ovary carcinoma labeled with Anti-VEGF Polyclonal Antibody, Unconjugated (ABIN727205) at 1:200, followed by conjugation to the secondary antibody and DAB staining

Antigen: 0.

Cells labeled with Anti-VEGF Polyclonal Antibody (ABIN727205).

HeLa cells probed with Rabbit Anti-VEGF Polyclonal Antibody Alexa Fluor 488 Conjugated.

Formalin-fixed and paraffin embedded: human ovary carcinoma labeled with Anti-VEGF Polyclonal Antibody, Unconjugated at 1:200, followed by conjugation to the secondary antibody and DAB staining

Human colon carcinoma lysates probed with Rabbit Anti-VEGF Polyclonal Antibody (ABIN727205) at 1:200 in 4 °C.

6 images

14 references

Catalog No. ABIN727205

TLR4 antibody (AA 751-835)

TLR4 Reactivity: Human, Mouse, Rat, Cow WB, FACS, ELISA, IHC (p), IF (p), IF (cc), IHC (fro) Host: Rabbit Polyclonal unconjugated

Mouse macrophages(RAW264.

Mouse splenocytes probed with Rabbit Anti-TLR4 Polyclonal Antibody .

Mouse macrophages(RAW264.

Image submitted by One World Lab validation program.

Formalin-fixed and paraffin embedded human placenta labeled with Rabbit Anti TLR4/CD284 Polyclonal Antibody, Unconjugated at 1:200 followed by conjugation to the secondary antibody and DAB staining

Formalin-fixed and paraffin embedded human placenta labeled with Rabbit Anti TLR4/CD284 Polyclonal Antibody, Unconjugated (ABIN736491) at 1:200 followed by conjugation to the secondary antibody and DAB staining

Formalin-fixed and paraffin embedded rat heart labeled with Rabbit Anti TLR4/CD284 Polyclonal Antibody, Unconjugated (ABIN736491) at 1:200 followed by conjugation to the secondary antibody and DAB staining

Mouse heart lysates probed with Anti TLR4/CD284 Polyclonal Antibody, Unconjugated (ABIN736491) at 1:200 overnight at 4 °C.

8 images

13 references

Catalog No. ABIN736491

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