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FACS Antibodies

Fluorescence-activated cell sorting (FACS) is a specialized type of flow cytometry. It sorts a heterogeneous mixture of biological cells into two or more containers, one cell at a time, based upon the specific light scattering and fluorescent characteristics of each cell and its attached antibodies.

Important Targets

Arginase 1 Antibodies

CASP8 Antibodies

CCR2 Antibodies

CD14 Antibodies

FOXP3 Antibodies

IL17 Antibodies

Lamin B1 Antibodies

All Flow Cytometry Antibodies

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97,105 results

Cadherin antibody (AA 841-882)

Reactivity: Human, Mouse WB, FACS, ICC, IF (cc), IF (p), IHC (p), ELISA, IHC (fro) Host: Rabbit Polyclonal unconjugated

Flow Cytometry validation image for anti-Cadherin (AA 841-882) antibody (ABIN687682)

Flow Cytometry validation image for anti-Cadherin (AA 841-882) antibody (ABIN687682)

Formalin-fixed and paraffin embedded mouse kidney labeled with Rabbit Anti E cadherin/CD324 Polyclonal Antibody, Unconjugated (ABIN687682) at 1:200 followed by conjugation to the secondary antibody and DAB staining

Formalin-fixed and paraffin embedded mouse intestine labeled with Rabbit Anti E cadherin/CD324 Polyclonal Antibody, Unconjugated (ABIN687682) at 1:200 followed by conjugation to the secondary antibody and DAB staining

4 images

2 references

Independent Validation

1 validation

Catalog No. ABIN687682

Independently Validated

LRP2 antibody (AA 3401-3500) (Cy3)

LRP2 Reactivity: Human, Mouse FACS, IF (cc), IF (p) Host: Rabbit Polyclonal Cy3

Immunofluorescence validation image for anti-Low Density Lipoprotein Receptor-Related Protein 2 (LRP2) (AA 3401-3500) antibody (Cy3) (ABIN750991)

Immunofluorescence validation image for anti-Low Density Lipoprotein Receptor-Related Protein 2 (LRP2) (AA 3401-3500) antibody (Cy3) (ABIN750991)

Immunofluorescence validation image for anti-Low Density Lipoprotein Receptor-Related Protein 2 (LRP2) (AA 3401-3500) antibody (Cy3) (ABIN750991)

Immunofluorescence validation image for anti-Low Density Lipoprotein Receptor-Related Protein 2 (LRP2) (AA 3401-3500) antibody (Cy3) (ABIN750991)

Immunofluorescence validation image for anti-Low Density Lipoprotein Receptor-Related Protein 2 (LRP2) (AA 3401-3500) antibody (Cy3) (ABIN750991)

5 images

Independent Validation

1 validation

Catalog No. ABIN750991

Independently Validated

TCF7L2 antibody

TCF7L2 Reactivity: Human, Mouse, Rat WB, IHC, IF (cc), IHC (p), FACS Host: Rabbit Monoclonal 2C2 unconjugated

Cleavage Under Targets and Release Using Nuclease validation image for anti-Transcription Factor 7-Like 2 (T-Cell Specific, HMG-Box) (TCF7L2) antibody (ABIN6945139)

Cleavage Under Targets and Release Using Nuclease validation image for anti-Transcription Factor 7-Like 2 (T-Cell Specific, HMG-Box) (TCF7L2) antibody (ABIN6945139)

Library profiles comparing fragment size distributions on an E-Gel EX 2% agarose gel (Thermo Fisher).

Alignment tracks from CUT&RUN targeting TCF7L2 in HEK293T cells using anti-TCF7L2 antibody ABIN6945139 (1).

4 images

1 reference

Independent Validation

1 validation

Catalog No. ABIN6945139

Independently Validated

Histone 3 antibody (AA 71-136)

H3 Reactivity: Human, Mouse, Rat WB, ELISA, ICC, FACS, IHC (p), IF (cc), IF (p), IHC (fro) Host: Rabbit Polyclonal unconjugated

K562 Cell lysates probed with Histone H3 Polyclonal Antibody, unconjugated at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 60 minutes at 37°C.

Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti Histone H3 Polyclonal Antibody, Unconjugated (ABIN739109) at 1:200 followed by conjugation to the secondary antibody and DAB staining

Mouse splenocytes probed with Rabbit Anti-Histone H3 Polyclonal Antibody, Unconjugated .

Paraformaldehyde-fixed, paraffin embedded human colon cancer, Antigen retrieval by boiling in sodium citrate buffer (pH6.

Formalin-fixed and paraffin-embedded rat brain labeled with Anti-Histone H3 Polyclonal Antibody, Unconjugated (ABIN739109) 1:200, overnight at 4°C, The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated used at 1:200 dilution for 40 minutes at 37°C.

Formalin-fixed and paraffin embedded mouse intestine labeled with Rabbit Anti Histone H3 Polyclonal Antibody, Unconjugated (ABIN739109) at 1:200 followed by conjugation to the secondary antibody and DAB staining

6 images

13 references

Catalog No. ABIN739109

NLRP3 antibody (AA 15-120)

NLRP3 Reactivity: Human, Mouse, Rat, Dog WB, ELISA, FACS, IHC (p), ICC, IHC (fro), IF (p), IF (cc) Host: Rabbit Polyclonal unconjugated

Decreased cAMP exaggerated acute gouty arthritis in P2Y14R-KO rats.

Antigen: 2 µg/100 µL Primary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; Secondary: HRP conjugated Rabbit Anti-Goat IgG at 1: 5000; TMB staining Read the data in Microplate Reader by 450nm.

Formalin-fixed and paraffin embedded mouse embryo labeled with Rabbit Anti Cryopyrin/CIAS1/NALP3 Polyclonal Antibody, Unconjugated (ABIN1386361) at 1:200 followed by conjugation to the secondary antibody and DAB staining

Formalin-fixed and paraffin embedded human lung carcinoma labeled with Rabbit Anti Cryopyrin/CIAS1/NALP3 Polyclonal Antibody, Unconjugated (ABIN1386361) at 1:200 followed by conjugation to the secondary antibody and DAB staining

This image was generously provided by Adib Zendedel, PhD from RWTH Aachen University.

Formalin-fixed and paraffin embedded mouse intestine labeled with Anti-Cryopyrin Polyclonal Antibody, Unconjugated at 1:200 followed by conjugation to the secondary antibody and DAB staining.

HeLa lysates probed with Rabbit Anti-Cryopyrin Polyclonal Antibody, Unconjugated at 1:5000 for 90 min at 37˚C.

Formalin-fixed and paraffin embedded rat kidney labeled with Anti-Cryopyrin Polyclonal Antibody, Unconjugated at 1:200 followed by conjugation to the secondary antibody and DAB staining.

Formalin-fixed and paraffin embedded mouse intestine labeled with Anti-Cryopyrin Polyclonal Antibody, Unconjugated at 1:200 followed by conjugation to the secondary antibody and DAB staining.

EPD inhibited the IMQ-activated nucleotide-binding oligomerization domain (Nod)-like receptor family pyrin domain-containing 3 (NLRP3) inflammasome signaling pathway.

EPD inhibited the IMQ-activated nucleotide-binding oligomerization domain (Nod)-like receptor family pyrin domain-containing 3 (NLRP3) inflammasome signaling pathway.

NLRP3 inflammasome activation was involved in P2Y14R deficiency.

NLRP3 inflammasome activation was involved in P2Y14R deficiency.

Upregulated cAMP suppressed acute gouty arthritis in vivo.

Pyroptosis stress was invalidated once cAMP increased by Forskolin in vitro.

15 images

11 references

Catalog No. ABIN1386361

ICAM1 antibody (AA 201-300)

ICAM1 Reactivity: Human, Mouse, Rat, Rabbit WB, FACS, ELISA, ICC, IHC (p), IHC (fro), IF (cc), IF (p) Host: Rabbit Polyclonal unconjugated

Formalin-fixed and paraffin embedded rat brain tissue labeled with Anti-CD54/ICAM-1 Polyclonal Antibody (ABIN670806), Unconjugated at 1:200, followed by conjugation to the secondary antibody and DAB staining

EPD inhibited inflammatory cell infiltration in IMQ-induced psoriasis-like mouse mode.

Formalin-fixed and paraffin embedded mouse heart tissue labeled with Anti-CD54 ICAM-1 Polyclonal Antibody, unconjugated (ABIN670806) at 1: 200,followed by incubation with conjugated secondary antibody (Goat Anti-Rabbit IgG, PE conjugated at 1: 200 for 40 minutes at 37°C).

Lane 1: rat brain lysates Lane 2: rat heart lysates probed with Anti CD54/ICAM-1 Polyclonal Antibody, Unconjugated (ABIN670806) at 1:200 in 4 °C.

Formalin-fixed and paraffin embedded mouse heart tissue labeled with Anti-CD54/ICAM-1 Polyclonal Antibody (ABIN670806), Unconjugated at 1:200, followed by conjugation to the secondary antibody Goat Anti-Rabbit IgG, FITC conjugated used at 1:200 dilution for 40 minutes at 37°C and DAPI staining

Formalin-fixed and paraffin embedded rat brain tissue labeled with Anti-CD54/ICAM-1 Polyclonal Antibody , Unconjugated at 1:200, followed by conjugation to the secondary antibody and DAB staining

6 images

16 references

Catalog No. ABIN670806

Cytochrome C antibody (AA 51-105)

CYCS Reactivity: Human, Rat, Mouse, Rabbit, Chicken WB, FACS, ELISA, ICC, IHC (p), IF (p), IHC (fro), IF (cc) Host: Rabbit Polyclonal unconjugated

Mouse heart probed with Anti-Cytochrome C Polyclonal Antibody Unconjugated at 1:5000 for 90 min at 37˚C.

L1 mouse brain lysates L2 mouse lung lysates probed with Anti Cytochrome C Polyclonal Antibody, Unconjugated (ABIN723590) at 1:200 overnight at 4 °C.

Image kindly submitted by an end-user via Funakoshi.

Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti-Cytochrome C Polyclonal Antibody, Unconjugated 1:300 followed by conjugation to the secondary antibody and DAB staining

HepG2 cells probed with Cytochrome C Polyclonal Antibody, unconjugated at 1:100 dilution for 30 minutes compared to control cells (blue) and isotype control (orange).

Elevation of Srx-1 attenuated SI/R-triggered mitochondrial apoptosis pathwayAfter transfection with Ad-Srx-1, cells were then exposure to SI/R.

Srx-1 enhanced cardiomyocyte resistance to SI/R injury by the PI3K/AKT signalling(A) Western blotting analysis for phospho-AKT and AKT in H9c2 cell treated with Ad-Srx-1 upon SI/R.

Analysis of activation of the intrinsic apoptotic pathway.

8 images

19 references

Catalog No. ABIN723590

VEGF antibody (AA 27-120)

VEGF Reactivity: Human, Mouse, Rat, Pig, Goat WB, ELISA, FACS, IHC (p), IHC (fro), IF (p) Host: Rabbit Polyclonal unconjugated

Formalin-fixed and paraffin embedded: human ovary carcinoma labeled with Anti-VEGF Polyclonal Antibody, Unconjugated (ABIN727205) at 1:200, followed by conjugation to the secondary antibody and DAB staining

Antigen: 0.

Cells labeled with Anti-VEGF Polyclonal Antibody (ABIN727205).

HeLa cells probed with Rabbit Anti-VEGF Polyclonal Antibody Alexa Fluor 488 Conjugated.

Formalin-fixed and paraffin embedded: human ovary carcinoma labeled with Anti-VEGF Polyclonal Antibody, Unconjugated at 1:200, followed by conjugation to the secondary antibody and DAB staining

Human colon carcinoma lysates probed with Rabbit Anti-VEGF Polyclonal Antibody (ABIN727205) at 1:200 in 4 °C.

6 images

14 references

Catalog No. ABIN727205

TLR4 antibody (AA 751-835)

TLR4 Reactivity: Human, Mouse, Rat, Cow WB, FACS, ELISA, IHC (p), IF (p), IF (cc), IHC (fro) Host: Rabbit Polyclonal unconjugated

Mouse macrophages(RAW264.

Mouse splenocytes probed with Rabbit Anti-TLR4 Polyclonal Antibody .

Mouse macrophages(RAW264.

Image submitted by One World Lab validation program.

Formalin-fixed and paraffin embedded human placenta labeled with Rabbit Anti TLR4/CD284 Polyclonal Antibody, Unconjugated at 1:200 followed by conjugation to the secondary antibody and DAB staining

Formalin-fixed and paraffin embedded human placenta labeled with Rabbit Anti TLR4/CD284 Polyclonal Antibody, Unconjugated (ABIN736491) at 1:200 followed by conjugation to the secondary antibody and DAB staining

Formalin-fixed and paraffin embedded rat heart labeled with Rabbit Anti TLR4/CD284 Polyclonal Antibody, Unconjugated (ABIN736491) at 1:200 followed by conjugation to the secondary antibody and DAB staining

Mouse heart lysates probed with Anti TLR4/CD284 Polyclonal Antibody, Unconjugated (ABIN736491) at 1:200 overnight at 4 °C.

8 images

13 references

Catalog No. ABIN736491

IL-1 beta antibody (AA 101-200)

IL1B Reactivity: Human, Mouse, Rat, Rabbit, Pig, Cow WB, ELISA, FACS, IF (p), IHC (p), IF (cc), IHC (fro), DB Host: Rabbit Polyclonal unconjugated

Raw264.

GSPIONs do not increase expression of pro-inflammatory cytokines in lung parenchyma.

Antigen: 2 µg/100 µL Primary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; Secondary: HRP conjugated Rabbit Anti-Goat IgG at 1: 5000; TMB staining Read the data in Microplate Reader by 450nm.

Formalin-fixed and paraffin embedded mouse lung labeled with (ABIN728503) Rabbit Anti-IL-1 Beta/IL-1B Polyclonal Antibody, Unconjugated followed by conjugation to the secondary antibody and DAB staining.

Image was kindly submitted by Dr.

EPD inhibited the IMQ-activated nucleotide-binding oligomerization domain (Nod)-like receptor family pyrin domain-containing 3 (NLRP3) inflammasome signaling pathway.

Effects of ALA on cardiac function, inflammation and autophagy in CLP-induced septic rats.

EPD inhibited the IMQ-activated nucleotide-binding oligomerization domain (Nod)-like receptor family pyrin domain-containing 3 (NLRP3) inflammasome signaling pathway.

8 images

16 references

Catalog No. ABIN728503

IL-6 antibody (AA 111-150)

IL6 Reactivity: Human, Mouse, Rat, Rabbit, Cow WB, ELISA, FACS, IF (p), IHC (p), IF (cc), IHC (fro) Host: Rabbit Polyclonal unconjugated

Protein expression of PPARγ, IL-6, and TNF-α in WAT after vibration for 8 weeks.

Antigen: 2 µg/100 µL Primary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; Secondary: HRP conjugated Rabbit Anti-Goat IgG at 1: 5000; TMB staining Read the data in Microplate Reader by 450nm.

Formalin-fixed and paraffin embedded mouse lung tissue labeled with Anti-IL-6(mo, rat) Polyclonal Antibody, Unconjugated (ABIN728083) at 1:200, followed by conjugation to the secondary antibody and DAB staining

L1 mouse lung lysates L2 protein provided by the end user lysates probed with Anti IL-6 Polyclonal Antibody, Unconjugated (ABIN728083) at 1:200 overnight at 4 °C.

Formalin-fixed and paraffin embedded rat liver tissue labeled with Anti-IL-6(mo, rat) Polyclonal Antibody, Unconjugated (ABIN728083) at 1:200, followed by conjugation to the secondary antibody and DAB staining

Formalin-fixed and paraffin embedded mouse lung tissue labeled with Anti-IL-6(mo, rat) Polyclonal Antibody, Unconjugated at 1:200, followed by conjugation to the secondary antibody and DAB staining

6 images

14 references

Catalog No. ABIN728083

GFAP antibody (AA 51-150)

GFAP Reactivity: Human, Rat, Mouse WB, ICC, ELISA, FACS, IHC (p), IHC (fro), IF (cc), IF (p) Host: Rabbit Polyclonal unconjugated

U251 Cells lysates probed with GFAP Polyclonal Antibody, unconjugated at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 60 minutes at 37°C.

Image provided by One World Lab validation program.

Formalin-fixed and paraffin embedded: rat brain tissue Anti-GFAP Polyclonal Antibody, Unconjugated at 1:400, followed by conjugation to the secondary antibody and DAB staining

Paraformaldehyde-fixed, paraffin embedded rat brain, Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30min, Antibody incubation with GFAP Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by a conjugated secondary -AF594) at 1:500 for 90 minutes and DAPI staining of the nuclei.

RSC96 cells probed with GFAP Polyclonal Antibody, Unconjugated at 1:100 for 30 minutes followed by incubation with a conjugated secondary (PE Conjugated) (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).

Image provided by One World Lab validation program.

Astrocytes were stained with GFAP polyclonal antibody .

Formalin-fixed and paraffin embedded mouse spinal cord stained with GFAP Polyclonal Antibody at 1:100 dilution.

8 images

20 references

Catalog No. ABIN726200

FGF2 antibody (AA 143-250)

FGF2 Reactivity: Human, Mouse, Rat, Rabbit, Sheep WB, ELISA, FACS, IHC (p), IF (p), IF (cc), IHC (fro) Host: Rabbit Polyclonal unconjugated

(a) Representative Masson staining images of the heart papillary muscle cross-sectional scan images, and infarct rate of the different groups.

Formalin-fixed and paraffin embedded: rat brain tissue labeled with Anti-bFGF Polyclonal Antibody, Unconjugated (ABIN726425) at 1:200, followed by conjugation to the secondary antibody and DAB staining

Formalin-fixed and paraffin embedded mouse kidney labeled with Anti-BFGF/FGF2 Polyclonal Antibody, Unconjugated (ABIN726425) followed by conjugation to the secondary antibody and DAB staining

Human HepG2 cells probed with Rabbit Anti-bFGF Polyclonal Antibody

Image provided by One World Lab validation program.

Formalin-fixed and paraffin embedded: rat brain tissue labeled with Anti-bFGF Polyclonal Antibody, Unconjugated at 1:200, followed by conjugation to the secondary antibody and DAB staining

Mouse spleen lysates probed with bFGF Polyclonal Antibody, Unconjugated at 1:300 dilution and 4˚C overnight incubation.

HepG2 lysates probed with bFGF Polyclonal Antibody, Unconjugated at 1:500 dilution and 4˚C overnight incubation.

(a) Representative images and bar graphs of the transmigrated cell number in the labeled and unlabeled endothelial progenitor cell (EPC) groups (x200).

9 images

18 references

Catalog No. ABIN726425

NF-kB p65 antibody (pSer536)

NFkBP65 Reactivity: Human, Mouse, Rat, Cow, Chicken WB, ELISA, IHC (p), FACS Host: Rabbit Polyclonal unconjugated

SeMet reversed activation of TLR4/NF-κB pathway induced by ESBL-E.

Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti-phospho-NFKB p65(Ser536) Polyclonal Antibody, Unconjugated (ABIN730528) at 1:200 followed by conjugation to the secondary antibody and DAB staining.

Mouse lung lysates probed with Rabbit Anti-NFkB p65 (Ser536) Polyclonal Antibody, Unconjugated at 1:5000 for 90 min at 37˚C.

Mouse spleen lysates probed with Rabbit Anti-NFKB p65(Ser536) Polyclonal Antibody, Unconjugated at 1:500 for 90 min at 37˚C

Mouse spleen lysates probed with Rabbit Anti-NFKB p65(Ser536) Polyclonal Antibody, Unconjugated at 1:5000 for 90 min at 37˚C.

HL-60 cells probed with NFKB p65(Ser536) Polyclonal Antibody, unconjugated at 1:100 dilution for 30 minutes compared to control cells (blue) and isotype control (orange)

Necrostatin-1 attenuated neuroinflammatory changes in a rat neuropathic pain model.

SeMet inhibited activation of TLR4/NF-κB pathway induced by ESBL-E.

8 images

12 references

Catalog No. ABIN730528

CXCR4 antibody (AA 201-294)

CXCR4 Reactivity: Human, Mouse, Rat, Rabbit WB, ELISA, FACS, IHC (p), IF (cc), IF (p), IHC (fro) Host: Rabbit Polyclonal unconjugated

Mouse lung lysates probed with Anti-CXCR4 Polyclonal Antibody at 1:5000 for 90 min at 37˚C.

L1 rat brain lysates L2 rat kidney lysates probed with Anti- CD184/CXCR4 Polyclonal Antibody, Unconjugated (ABIN730888) at 1:200 overnight at 4 °C.

Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti-CD184/CXCR4 Polyclonal Antibody (ABIN730888), Unconjugated at 1:200, followed by conjugation to the secondary antibody and DAB staining

Formalin-fixed and paraffin embedded human breast carcinoma labeled with Anti-CD184/CXCR4 Polyclonal Antibody , Unconjugated at 1:200, followed by conjugation to the secondary antibody and DAB staining

Human U937 cells probed withCXCR4 Polyclonal Antibody, Unconjugated at 1:100 for 30 minutes followed by incubation with a PE conjugated secondary (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).

Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti-CD184/CXCR4 Polyclonal Antibody , Unconjugated at 1:200, followed by conjugation to the secondary antibody and DAB staining

Formalin-fixed and paraffin embedded human breast carcinoma labeled with Anti-CD184/CXCR4 Polyclonal Antibody (ABIN730888), Unconjugated at 1:200, followed by conjugation to the secondary antibody and DAB staining

Mouse splenocytes probed with Rabbit Anti-CXCR4 Polyclonal Antibody .

8 images

16 references

Catalog No. ABIN730888

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