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Polyclonal Antibodies

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CD38 antibody (C-Term)

Reactivity: Human FACS, WB, IF, IHC (p) Host: Rabbit Polyclonal RB18001 unconjugated

FACS

Immunofluorescence analysis of CD38 Antibody (C-term) with paraffin-embedded human hepatocarcinoma tissue.

Confocal immunofluorescent analysis of CD38 Antibody (C-term) (ABIN389405 and ABIN2839493) with Hela cell followed by Alexa Fluor® 488-conjugated goat anti-rabbit lgG (green).

Western blot analysis of lysate from 293 cell lysates (2 μg/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the CD38 gene using CD38 Antibody (C-term) (ABIN389405 and ABIN2839493).

Western blot analysis of lysate from R cell line, using CD38 Antibody (C-term) (ABIN389405 and ABIN2839493).

Formalin-fixed and paraffin-embedded human hepatocarcinoma with CD38 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

IHC (p)

6 images

Catalog No. ABIN389405

$451.00

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UCHL1 antibody (N-Term)

UCHL1 Reactivity: Human, Mouse WB, IF, FACS, IHC (p) Host: Rabbit Polyclonal RB04183 unconjugated

All lanes : Anti-UCHL1 Antibody (V31) at 1:1000 dilution Lane 1: D whole cell lysate Lane 2: SH-SY5Y whole cell lysate Lysates/proteins at 20 μg per lane.

Formalin-fixed and paraffin-embedded human brain tissue reacted with UCHL1 (Park5) antibody (N-term) (ABIN388868 and ABIN2839164) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

IHC (p)

Confocal immunofluorescent analysis of UCHL1 Antibody (N-term) (ABIN388868 and ABIN2839164) with NCI- cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).

Western blot analysis of UCHL1 (arrow) using rabbit polyclonal UCHL1-V31 (ABIN388868 and ABIN2839164).

Western blot analysis of UCHL1-V31 (ABIN388868 and ABIN2839164) in CEM(lane 1), Jurkat(lane 2), Y79(lane 3) cell line and mouse brain tissue(lane 4) lysates (35 μg/lane).

UCHL1 Antibody (N-term) (ABIN388868 and ABIN2839164) flow cytometric analysis of NCI- cells (right histogram) compared to a negative control cell (left histogram).

FACS

6 images

Catalog No. ABIN388868

$451.00

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Vimentin antibody (C-Term)

VIM Reactivity: Human WB, IF, FACS, IHC (p) Host: Rabbit Polyclonal RB16691 unconjugated

Vimentin Antibody (C-term) (ABIN389212 and ABIN2839367) immunohistochemistry analysis in formalin fixed and paraffin embedded human colon tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

IHC (p)

Fluorescent confocal image of SY5Y cells stained with Vimentin (C-term) antibody.

Western blot analysis of Vimentin antibody (C-term) (ABIN389212 and ABIN2839367) in NCI- cell line lysates (35 μg/lane).

Vimentin Antibody (C-term) (ABIN389212 and ABIN2839367) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).

FACS

5 images

2 references

Catalog No. ABIN389212

$451.00

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Cyclin D1 antibody (AA 68-97)

CCND1 Reactivity: Human, Mouse WB, IF, IHC (p) Host: Rabbit Polyclonal RB8066 unconjugated

Western blot analysis of anti-Cyclin D1 Antibody (S90) (ABIN389146 and ABIN2850438) in mouse lung lysates (35 μg/lane).

IHC (p)

Confocal immunofluorescent analysis of Cyclin D1 Antibody (S90) (ABIN389146 and ABIN2850438) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).

All lanes : Anti-Cyclin D1 Antibody (S90) at 1:2000 dilution Lane 1: MCF-7 whole cell lysate Lane 2: A431 whole cell lysate Lysates/proteins at 20 μg per lane.

All lanes : Anti-Cyclin D1 Antibody (S90) at 1:2000 dilution Lane 1: HT-1080 whole cell lysate Lane 2: MCF-7 whole cell lysate Lysates/proteins at 20 μg per lane.

5 images

1 reference

Catalog No. ABIN389146

$451.00

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LGR5 antibody (AA 443-473)

LGR5 Reactivity: Human WB, FACS, IHC (p) Host: Rabbit Polyclonal RB14555 unconjugated

Western blot analysis of anti-LGR5/GPR49 Antibody (Center) f in K562 cell line lysates (35 μg/lane).

LGR5 (GPR49) Antibody (Center) f flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).

FACS

LGR5/GPR49 Antibody (Center) f western blot analysis in HepG2 cell line lysates (35 μg/lane).

Immunohistochemical analysis of paraffin-embedded Human brain section using Pink1 F.

IHC

Formalin-fixed and paraffin-embedded human brain tissue reacted with LGR5/GPR49 antibody (Center) f , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

IHC (p)

5 images

2 references

Catalog No. ABIN389219

$451.00

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KLF4 antibody (AA 69-101)

KLF4 Reactivity: Human WB, IF, IHC (p) Host: Rabbit Polyclonal RB14263 unconjugated

Anti-KLF4 Antibody (N-term C74) at 1:2000 dilution + NCCIT whole cell lysate Lysates/proteins at 20 μg per lane.

Fluorescent confocal image of HeLa cells stained with (ABIN389187 and ABIN2839350) KLF4 (N-term C74) antibody.

Formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with KLF4 antibody (N-term C74) (ABIN389187 and ABIN2839350) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

IHC (p)

Fluorescent confocal image of SY5Y cells stained with (ABIN389187 and ABIN2839350) KLF4 (N-term C74) antibody.

Formalin-fixed and paraffin-embedded human Spleen tissue reacted with KLF4 antibody (N-term C74) (ABIN389187 and ABIN2839350) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.

IHC (p)

5 images

1 reference

Catalog No. ABIN389187

$451.00

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Neurogenin 3 antibody (N-Term)

NEUROG3 Reactivity: Human, Mouse WB, IF, IHC (p) Host: Rabbit Polyclonal RB02913 unconjugated

IHC (p)

Confocal immunofluorescent analysis of Neurogenin3 Antibody (N-term) (ABIN388770 and ABIN2839081) with NCI- cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).

Anti-Neurogenin3 Antibody (N-term) at 1:500 dilution + Human placenta tissue lysate Lysates/proteins at 20 μg per lane.

Anti-Neurogenin3 Antibody (N-term) at 1:2000 dilution + THP-1 whole cell lysate Lysates/proteins at 20 μg per lane.

IHC (p)

6 images

Catalog No. ABIN388770

$451.00

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Beclin 1 antibody (AA 210-239)

BECN1 Reactivity: Human, Mouse WB, FACS, IHC (p) Host: Rabbit Polyclonal RB7463 unconjugated

All lanes : Anti-Beclin-1 Antibody at 1:4000 dilution Lane 1: Hela whole cell lysate Lane 2: A431 whole cell lysate Lysates/proteins at 20 μg per lane.

Flow cytometric analysis of Y79 cells using Beclin 1 (BECN1/G6) Antibody (bottom histogram) compared to a negative control cell (top histogram).

FACS

Western blot analysis of anti-hBECN1- Pab (ABIN388546 and ABIN2849692) in 293 cell line lysates transiently transfected with the BECN1 gene (2 μg/lane).

The anti-BECN1 Pab (ABIN388546 and ABIN2849692) is used in Western blot to detect BECN1 in, from left to right, , Hela, mouse brain, Y79, and HL60 tissue lysates.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

IHC (p)

5 images

6 references

Catalog No. ABIN388546

$451.00

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SOX2 antibody (N-Term)

SOX2 Reactivity: Human WB, IF, FACS, IHC (p) Host: Rabbit Polyclonal RB14026 unconjugated

Flow cytometric analysis of NCI- cells using SOX2 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram).

FACS

Immunofluorescence analysis of SOX2 Antibody (N-term) (ABIN388797 and ABIN2839124) in HeLa cells.

Western blot analysis of SOX2 (arrow) using rabbit polyclonal SOX2 Antibody (N-term) (ABIN388797 and ABIN2839124).

Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with SOX2 antibody (N-term) (ABIN388797 and ABIN2839124) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

IHC (p)

5 images

2 references

Catalog No. ABIN388797

$451.00

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Cadherin 4 antibody (N-Term)

CDH4 Reactivity: Human WB, IF, FACS, IHC (p) Host: Rabbit Polyclonal RB13654 unconjugated

All lanes : Anti-CDH4 Antibody (N-term) at 1:2000 dilution Lane 1: A431 whole cell lysate Lane 2: SH-SY5Y whole cell lysate Lysates/proteins at 20 μg per lane.

Flow cytometric analysis of HepG2 cells using CDH4 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram).

FACS

Immunofluorescence analysis of CDH4 Antibody (N-term) Antibody with paraffin-embedded human brain tissue.

Anti-CDH4 Antibody (N-term) at 1:2000 dilution + HepG2 whole cell lysate Lysates/proteins at 20 μg per lane.

Formalin-fixed and paraffin-embedded human brain tissue reacted with CDH4 antibody (N-term) (ABIN388148 and ABIN2846884) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

IHC (p)

5 images

1 reference

Catalog No. ABIN388148

$451.00

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GABARAP antibody (AA 1-30)

GABARAP Reactivity: Human, Mouse, Rat WB, IF, IHC (p) Host: Rabbit Polyclonal RB11846 unconjugated

All lanes : Anti-Autophagy GABAR Antibody (N-term) at 1:1000-1 :2000 dilution Lane 1: human fetal kidney lysate Lane 2: SH-SY5Y whole cell lysate Lane 3: mouse brain lysate Lane 4: rat brain whole cell lysate Lane 5: HepG2 whole cell lysate Lane 6: human fetal brain lysate Lane 7: C2C12 whole cell lysate Lysates/proteins at 20 μg per lane.

IHC (p)

Immunofluorescent analysis of 4 % paraformaldehyde-fixed, 0.

IHC (p)

5 images

4 references

Catalog No. ABIN388564

$451.00

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CD90 antibody (N-Term)

THY1 Reactivity: Human WB, IHC (p) Host: Rabbit Polyclonal RB03970 unconjugated

Western blot analysis of anti-THY1 (N-term) Pab (ABIN388803 and ABIN2839126) pre-incubated without(lane 1) and with(lane 2) blocking peptide (BP2050a) in T47D cell line lysate.

Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with THY1 antibody (N-term) (ABIN388803 and ABIN2839126) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

IHC (p)

The anti-THY1 (N-term) Pab (ABIN388803 and ABIN2839126) is used in Western blot to detect THY1 in HL60 cell lysate.

IHC (p)

5 images

4 references

Catalog No. ABIN388803

$451.00

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ATG16L1 antibody (AA 161-190)

ATG16L1 Reactivity: Human, Mouse WB, IF, IHC (p) Host: Rabbit Polyclonal RB07490 unconjugated

G16L Antibody 1817b western blot analysis in NCI- cell line lysates (35 μg/lane).

Formalin-fixed and paraffin-embedded human colon carcinoma tissue reacted with Autophagy G16L antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

IHC (p)

Anti-ATG16L Antibody at 1:1000 dilution + Jurkat whole cell lysate Lysates/proteins at 20 μg per lane.

Western blot of G16L (L92) Pab 1817b in mouse brain tissue lysate.

Immunofluorescent analysis of cells, using ATG16L Antibody (ABIN388542 and ABIN2849682).

5 images

8 references

Catalog No. ABIN388542

$451.00

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HA-Tag antibody

WB, IF, ChIP Host: Rabbit Polyclonal RB0411 unconjugated

Western blot analysis of lysate from 12 tag recombinant protein cell line, using Tag-HA.

GTPase activity of dynamin-2 is required for endocytosis of cell-surface tTG.

ChIP

4 images

22 references

Catalog No. ABIN387848

$451.00

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Bcl-2 antibody (AA 75-110)

BCL2 Reactivity: Human WB, IF, IHC (p) Host: Rabbit Polyclonal RB04684 unconjugated

All lanes : Anti-Bcl-2 Antibody (BH3 Domain Specific) at 1:1000 dilution Lane 1: RI 8226 whole cell lysate Lane 2: Jurkat whole cell lysate Lane 3: THP-1 whole cell lysate Lysates/proteins at 20 μg per lane.

Immunohistochemical analysis of (ABIN388095 and ABIN2846147) on paraffin-embedded Human placenta tissue.

IHC (p)

Confocal immunofluorescent analysis of Bcl-2 Antibody (BH3 Domain Specific) (ABIN388095 and ABIN2846147) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).

Immunohistochemical analysis of (ABIN388095 and ABIN2846147) on paraffin-embedded Human tonsil tissue.

IHC (p)

4 images

21 references

Catalog No. ABIN388095

$451.00

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ATG7 antibody (C-Term)

ATG7 Reactivity: Human, Mouse WB, IF, IHC (p) Host: Rabbit Polyclonal RB7474 unconjugated

Western blot analysis of G7L Pab 1813d in 293 cell line lysate (35 μg/lane).

Immunohistochemical analysis of paraffin-embedded human liver tissue using D performed on the Leica® BOND RXm.

IHC (p)

All lanes : Anti-ATG7 Antibody (C-term) at 1:1000 dilution Lane 1: HepG2 whole cell lysate Lane 2: NIH/3T3 whole cell lysate Lysates/proteins at 20 μg per lane.

All lanes : Anti-ATG7 Antibody (C-term) at 1:500 dilution Lane 1: Caki-1 whole cell lysate Lane 2: Hela whole cell lysate Lane 3: HepG2 whole cell lysate Lysates/proteins at 40 μg per lane.

Immunofluorescent analysis of cells, using ATG7 Antibody (C-term) (ABIN388523 and ABIN2849643).

5 images

2 references

Catalog No. ABIN388523

$451.00

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HDAC9 antibody (N-Term)

HDAC9 Reactivity: Human WB, IF, IP, IHC (p) Host: Rabbit Polyclonal RB2582 unconjugated

Both anti-HDAC9 N-term ((ABIN387960 and ABIN2844679)) and C-term ((ABIN387961 and ABIN2844680)) Pab were tested by WB and IP-WB using HeLa and HeLa-HDAC9 transfected cells.

IHC (p)

Figure 2: Immunofluorescence staining of MITR for a compartmentalization study in undifferentiated C2C12 myoblasts transfected with a MITR-expressing plasmid.

This figure shows that both Pab can immunoprecipitate (IP) HDAC9 from HeLa-HDAC9 tranfected cells.

Figure 1: Immunoblots for MITR ((ABIN387960 and ABIN2844679) HDAC9 N-term antibody), Mirk, MyoD and tubulin proteins are shown for cytoplasmic (Cyt) and nuclear (N) extracts from undifferentiated C2C12 myoblasts.

5 images

3 references

Catalog No. ABIN387960

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BAX antibody (AA 41-76)

BAX Reactivity: Human, Mouse WB, FACS Host: Rabbit Polyclonal RB0952 unconjugated

All lanes : Anti-Bax Antibody (BH3 Domain Specific) at 1:2000 dilution Lane 1: Raji whole cell lysate Lane 2: SH-SH5Y whole cell lysate Lane 3: HT-1080 whole cell lysate Lane 4: NIH/3T3 whole cell lysate Lane 5: whole cell lysate Lysates/proteins at 20 μg per lane.

Overlay histogram showing HeLa cells stained with (ABIN388094 and ABIN2837860)(green line).

FACS

All lanes : Anti-Bax Antibody (BH3) at 1:2000 dilution Lane 1: HT-1080 whole cell lysate Lane 2: whole cell lysate Lane 3: Raji whole cell lysate Lane 4: SH-SY5Y whole cell lysate Lysates/proteins at 20 μg per lane.

All lanes : Anti-Bax Antibody (BH3 Domain Specific) at 1:2000 dilution Lane 1: HT-1080 whole cell lysate Lane 2: Raji whole cell lysate Lane 3: SH-SY5Y whole cell lysate Lane 4: THP-1 whole cell lysate Lane 5: NIH/3T3 whole cell lysate Lane 6: whole cell lysate Lysates/proteins at 20 μg per lane.

Anti-Bax Antibody (BH3) at 1:2000 dilution + mouse spleen lysate Lysates/proteins at 20 μg per lane.

5 images

8 references

Catalog No. ABIN388094

$451.00

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SLC29A1 antibody (C-Term)

SLC29A1 Reactivity: Human, Mouse WB, IHC (p), FACS Host: Rabbit Polyclonal RB09157 unconjugated

All lanes : Anti-ENT1 Antibody (C-term) at 1:1000 dilution Lane 1: Human heart lysate Lane 2: Human breast lysate Lane 3: Human placenta lysate Lysates/proteins at 20 μg per lane.

Overlay histogram showing HepG2 cells stained with B(green line).

FACS

All lanes : Anti-ENT1 Antibody (C-term) at 1:1000 dilution Lane 1: Human brain lysate Lane 2: Human breast lysate Lane 3: Human placenta lysate Lysates/proteins at 20 μg per lane.

All lanes : Anti-ENT1 Antibody (C-term) at 1:1000 dilution Lane 1: Mouse heart lysate Lane 2: Mouse liver lysate Lysates/proteins at 20 μg per lane.

Immunohistochemical analysis of paraffin-embedded human brain tissue using (ABIN387940 and ABIN2844497) performed on the Leica® BOND RXm.

IHC (p)

5 images

3 references

Catalog No. ABIN387940

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ACVRL1 antibody (N-Term)

ACVRL1 Reactivity: Human, Mouse WB, FACS, IHC (p) Host: Rabbit Polyclonal RB03736 unconjugated

human chondrocytes (C28/I2 cells), transfected with empty vector (lane 1, 3) or ACVRL1(lane 2, 4).

Flow cytometric analysis of HepG2 cells using ACVRL1 Antibody (N-term) (bottom histogram) compared to a negative control cell (top histogram).

FACS

Western blot of ACVRL1 Pab (ABIN392241 and ABIN2841932).

Western blot analysis of ACVRL1 (arrow) using rabbit polyclonal ACVRL1 Antibody (N-term) (ABIN392241 and ABIN2841932).

IHC (p)

5 images

4 references

Catalog No. ABIN392241

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DUSP6 antibody (AA 58-89)

DUSP6 Reactivity: Human, Mouse, Rat WB, IF, IHC (p) Host: Rabbit Polyclonal RB05814 unconjugated

All lanes : Anti-DUSP6 Antibody (Center) at 1:2000 dilution Lane 1: HepG2 whole cell lysate Lane 2: Human brain tissue lysate Lane 3: Rat brain tissue lysate Lysates/proteins at 20 μg per lane.

Immunohistochemical analysis of paraffin-embedded human kidney tissue using (ABIN392864 and ABIN2842279) performed on the Leica® BOND RXm.

IHC (p)

Confocal immunofluorescent analysis of DUSP6 Antibody (Center) (ABIN392864 and ABIN2842279) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).

All lanes : Anti-DUSP6 Antibody (Center) at 1:500 dilution Lane 1: Human brain tissue lysate Lane 2: Mouse brain tissue lysate Lane 3: Rat brain tissue lysate Lysates/proteins at 20 μg per lane.

All lanes : Anti-DUSP6 Antibody (Center) at 1:2000 dilution Lane 1: Human brain lysate Lane 2: Human lung lysate Lane 3: Rat brain lysate Lysates/proteins at 20 μg per lane.

Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using (ABIN392864 and ABIN2842279) performed on the Leica® BOND RXm.

IHC (p)

6 images

Catalog No. ABIN392864

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PIM2 antibody (C-Term)

PIM2 Reactivity: Human WB, IP, IHC (p) Host: Rabbit Polyclonal RB01288 unconjugated

Western blot analysis of PIM2 (arrow) using rabbit polyclonal PIM2 Antibody (ABIN392442 and ABIN2842040).

IHC (p)

PIM proteins were immunoprecipitated from MV4,11 cells and the agarose-protein A-immunoprecipitate complex was tested for its ability to phosphorylate BAD in vitro in the presence or absence of .

Western blot analysis of anti-PIM2 Antibody (C-term) (ABIN392442 and ABIN2842040) in Hela cell line lysates (35 μg/lane).

All lanes : Anti-PIM2 Antibody at 1:2000 dilution Lane 1: K562 whole cell lysate Lane 2: S whole cell lysate Lysates/proteins at 20 μg per lane.

5 images

1 reference

Catalog No. ABIN392442

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AGXT antibody (AA 96-125)

AGXT Reactivity: Human WB, FACS, IHC (p) Host: Rabbit Polyclonal RB18848 unconjugated

Anti-AGXT Antibody (Center)at 1:8000 dilution + human liver lysates Lysates/proteins at 20 μg per lane.

IHC (p)

Anti-AGXT Antibody (Center)at 1:2000 dilution + human liver lysates Lysates/proteins at 20 μg per lane.

Western blot analysis of AGXT Antibody (Center) (ABIN390393 and ABIN2840785) in HepG2 cell line lysates (35 μg/lane).

AGXT Antibody (Center) (R) IHC analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.

IHC (p)

7 images

Catalog No. ABIN390393

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LC3C antibody (pSer12)

MAP1LC3C Reactivity: Human WB, DB Host: Rabbit Polyclonal RB10838-RB16769 unconjugated

Immunoblots of phosphorylated LC3 (phospho-LC3) in CHO cell culture.

Dot blot analysis of Phospho-LC3 (G8a) - S12 Antibody 3301a and Nonphospho-LC3 (G8a) Antibody on nitrocellulose membrane.

Immunoblots of SH-SY5Y cells treated with rapamycin for 1 h was probed with (ABIN389699 and ABIN2839660).

Immunoblots of SH-SY5Y cells treated with P+ for 24h was probed with (ABIN389699 and ABIN2839660).

Something like SH-SY5Y cells expressing GFP-LC3-WT or-S12D treated with rapamycin or vehicle for 1h.

5 images

6 references

Catalog No. ABIN389699

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TBK1 antibody (AA 150-181)

TBK1 Reactivity: Human, Mouse WB, IHC (p) Host: Rabbit Polyclonal RB16573 unconjugated

All lanes : Anti-TBK1 Antibody at 1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: HT-1080 whole cell lysate Lane 3: K562 whole cell lysate Lane 4: MCF-7 whole cell lysate Lane 5: THP-1 whole cell lysate Lane 6: mouse testis lysate Lysates/proteins at 20 μg per lane.

Formalin-fixed and paraffin-embedded human testis tissue reacted with TBK-p, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

IHC (p)

All lanes : Anti-TBK Antibody at 1:2000 dilution Lane 1: HT-1080 whole cell lysate Lane 2: K562 whole cell lysate Lane 3: MCF-7 whole cell lysate Lysates/proteins at 20 μg per lane.

IHC (p)

5 images

1 reference

Catalog No. ABIN392371

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