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Western Blot Antibodies

Western blot (WB) antibodies are optimized for the detection of denatured proteins and validated to deliver clear, specific bands at the expected molecular weight. Use the filters below to quickly identify primary antibodies matched to your target, species, and experimental requirements.

Western Blot Antibody Validation

Antibodies for Western blot must reliably detect target proteins under denaturing conditions. Products in this category are validated for WB applications and selected for specific band detection at the expected molecular weight. Where available, validation data include experimental images, independent testing, and literature references to support antibody performance.

Western Blot Considerations

Protein denaturation during Western blotting can alter epitope structure, making antibody selection critical. Antibodies validated for WB are optimized to recognize linearized epitopes and reduce non-specific binding.

Monoclonal antibodies provide high specificity and consistent results, while polyclonal antibodies can offer stronger signal due to multi-epitope recognition. Recombinant antibodies combine defined specificity with improved reproducibility across experiments.

Western Blotting: Principles, Protocols, and Troubleshooting

Learn more about Western blotting, including protocols and troubleshooting

Use the filters below to narrow down Western blot antibodies by target, species, and experimental requirements.

529,062 results

STIM1 Reactivity: Human WB, ELISA, IF, IHC (p) Host: Mouse Monoclonal 5A2 unconjugated
Pubmed 20 references
Catalog No. ABIN563045
$520.15
Plus shipping costs $50.00
100 μg
Shipping to: United States
Delivery in 11 to 14 Business Days
 
TEAD4 Reactivity: Human WB, ELISA, IP, RNAi Host: Mouse Monoclonal 5H3 unconjugated
Pubmed 3 references
Catalog No. ABIN563135
$520.15
Plus shipping costs $50.00
100 μg
Shipping to: United States
Delivery in 11 to 14 Business Days
 
TOP1 Reactivity: Human WB, ELISA, IHC (p) Host: Mouse Monoclonal 1A1 unconjugated
Pubmed 1 reference
Catalog No. ABIN563222
$520.15
Plus shipping costs $50.00
100 μg
Shipping to: United States
Delivery in 11 to 14 Business Days
 
TNNI3 Reactivity: Human WB, ELISA, IF, IHC (p), RNAi Host: Mouse Monoclonal 1E7 unconjugated
Pubmed 1 reference
Catalog No. ABIN563216
$520.15
Plus shipping costs $50.00
100 μg
Shipping to: United States
Delivery in 11 to 14 Business Days
 
TSG101 Reactivity: Human WB, ELISA, IF, IHC (p), RNAi Host: Mouse Monoclonal 5B7 unconjugated
Pubmed 2 references
Catalog No. ABIN563269
$520.15
Plus shipping costs $50.00
100 μg
Shipping to: United States
Delivery in 11 to 14 Business Days
 
TWIST1 Reactivity: Human WB, ELISA, IP, RNAi Host: Mouse Monoclonal 3E11 unconjugated
Pubmed 3 references
Catalog No. ABIN563296
$520.15
Plus shipping costs $50.00
100 μg
Shipping to: United States
Delivery in 11 to 14 Business Days
 
TXN Reactivity: Human WB, ELISA, IP, IF Host: Mouse Monoclonal 2A7 unconjugated
Pubmed 1 reference
Catalog No. ABIN563300
$520.15
Plus shipping costs $50.00
100 μg
Shipping to: United States
Delivery in 11 to 14 Business Days
 
ube3a Reactivity: Human WB, ELISA, IF, IHC (p) Host: Mouse Monoclonal 2F6 unconjugated
Pubmed 1 reference
Catalog No. ABIN563326
$520.15
Plus shipping costs $50.00
100 μg
Shipping to: United States
Delivery in 11 to 14 Business Days
 
CCS Reactivity: Human WB, ELISA, IP, IHC (p), IF Host: Mouse Monoclonal 1E2 unconjugated
Pubmed 1 reference
Catalog No. ABIN523352
$520.15
Plus shipping costs $50.00
100 μg
Shipping to: United States
Delivery in 11 to 14 Business Days
 
POU5F1 Reactivity: Human WB, ELISA, IF Host: Mouse Monoclonal 1B11 unconjugated
Pubmed 1 reference
Catalog No. ABIN519056
$520.15
Plus shipping costs $50.00
100 μg
Shipping to: United States
Delivery in 11 to 14 Business Days
 
NR4A2 Reactivity: Human WB, ELISA, IF Host: Mouse Monoclonal 4A6 unconjugated
Pubmed 1 reference
Catalog No. ABIN518477
$520.15
Plus shipping costs $50.00
100 μg
Shipping to: United States
Delivery in 11 to 14 Business Days
 
ODC1 Reactivity: Human WB, IF, ELISA, IP Host: Mouse Monoclonal 2G5 unconjugated
Pubmed 1 reference
Catalog No. ABIN518505
$520.15
Plus shipping costs $50.00
100 μg
Shipping to: United States
Delivery in 11 to 14 Business Days
 
TPT1 Reactivity: Human WB, ELISA Host: Mouse Monoclonal 2C4 unconjugated
Pubmed 1 reference
Catalog No. ABIN521072
$520.15
Plus shipping costs $50.00
100 μg
Shipping to: United States
Delivery in 11 to 14 Business Days
 
CXCR4 Reactivity: Human WB, ELISA Host: Mouse Monoclonal 2G9 unconjugated
Pubmed 1 reference
Catalog No. ABIN521674
$520.15
Plus shipping costs $50.00
100 μg
Shipping to: United States
Delivery in 11 to 14 Business Days
 
RIPK2 Reactivity: Human WB, ELISA, IHC (p) Host: Mouse Monoclonal 6F7 unconjugated
Pubmed 1 reference
Catalog No. ABIN522206
$520.15
Plus shipping costs $50.00
100 μg
Shipping to: United States
Delivery in 11 to 14 Business Days
 
SPHK1 Reactivity: Human WB, ELISA Host: Mouse Monoclonal 1D6 unconjugated
Pubmed 11 references
Catalog No. ABIN522355
$520.15
Plus shipping costs $50.00
100 μg
Shipping to: United States
Delivery in 11 to 14 Business Days
 
EIF2AK2 Reactivity: Human WB, ELISA, IF, IHC (p) Host: Mouse Monoclonal 1D11 unconjugated
Pubmed 3 references
Catalog No. ABIN519273
$520.15
Plus shipping costs $50.00
100 μg
Shipping to: United States
Delivery in 11 to 14 Business Days
 
PI3K p85a Reactivity: Human WB, ELISA, IP, PLA Host: Mouse Monoclonal 3A10 unconjugated
Pubmed 1 reference
Catalog No. ABIN518903
$520.15
Plus shipping costs $50.00
100 μg
Shipping to: United States
Delivery in 11 to 14 Business Days
 
TGM2 Reactivity: Human WB, ELISA, IF, IHC (p) Host: Mouse Monoclonal 2F4 unconjugated
Pubmed 1 reference
Catalog No. ABIN520891
$520.15
Plus shipping costs $50.00
100 μg
Shipping to: United States
Delivery in 11 to 14 Business Days
 
TAZ Reactivity: Human WB, ELISA Host: Mouse Monoclonal 1B10 unconjugated
Pubmed 3 references
Catalog No. ABIN520708
$520.15
Plus shipping costs $50.00
100 μg
Shipping to: United States
Delivery in 11 to 14 Business Days
 
SMAD1 Reactivity: Human WB, ELISA, IF, IHC (p) Host: Mouse Monoclonal 2E9 unconjugated
Pubmed 1 reference
Catalog No. ABIN517610
$520.15
Plus shipping costs $50.00
100 μg
Shipping to: United States
Delivery in 11 to 14 Business Days
 
SMAD2 Reactivity: Human WB, ELISA, IF, IHC (p) Host: Mouse Monoclonal 3G9 unconjugated
Pubmed 1 reference
Catalog No. ABIN517619
$520.15
Plus shipping costs $50.00
100 μg
Shipping to: United States
Delivery in 11 to 14 Business Days
 
NME2 Reactivity: Human WB, ELISA, IF, IHC (p) Host: Mouse Monoclonal 1D3 unconjugated
Pubmed 1 reference
Catalog No. ABIN518376
$520.15
Plus shipping costs $50.00
100 μg
Shipping to: United States
Delivery in 11 to 14 Business Days
 
PRDX3 Reactivity: Human WB, IF Host: Mouse Polyclonal unconjugated
Pubmed 1 reference
Catalog No. ABIN524343
$520.15
Plus shipping costs $50.00
50 μL
Shipping to: United States
Delivery in 11 to 14 Business Days
 
CPSF6 Reactivity: Human WB, ELISA, IF Host: Mouse Monoclonal 3F11 unconjugated
Pubmed 2 references
Catalog No. ABIN524445
$520.15
Plus shipping costs $50.00
100 μg
Shipping to: United States
Delivery in 11 to 14 Business Days
 
  • Type Primary
    • Primary
  • Target
    • Histone 3 (H3)
    • p53 (TP53)
    • EGFR
    • tau
    • AKT1
    • NF-kB p65 (NFkBP65)
    • Estrogen Receptor alpha (ESR1)
    • Bcl-2 (BCL2)
    • KIT
    • TNF alpha
    • RAF1
    • c-MYC (MYC)
    • FAK (PTK2)
    • Vimentin (VIM)
    • GFAP
    • RPS6KB1
    • HSP27 (HSPB1)
    • ErbB2/Her2
    • Cytokeratin 18 (KRT18)
    • HSP70
    • Albumin (ALB)
    • CDK1
    • C-JUN (JUN)
    • IL-6 (IL6)
    • Histone H4 (HISTH4)
    • ATF2
    • STAT3
    • EpCAM (EPCAM)
    • SMAD2
    • Caspase 9 (CASP9)
    • GAPDH
    • SNCA
    • Cytokeratin 19 (KRT19)
    • MEK1 (MAP2K1)
    • Interferon gamma (IFNG)
    • beta Catenin (CATNB)
    • E-cadherin (CDH1)
    • Caspase 3 (CASP3)
    • CREB1
    • HSPD1
    • CD31 (PECAM1)
    • Histone H2B
    • SOD1
    • BAD
    • GRP78 (HSPA5)
    • ERK1 (MAPK3)
    • Src
    • CD45 (PTPRC)
    • eIF4EBP1 (EIF4EBP1)
    • FGFR1
    Please use search to find more...
  • Application Western Blotting (WB)
    • Western Blotting (WB)
    • ELISA
    • Immunohistochemistry (IHC)
    • Immunofluorescence (Paraffin-embedded Sections) (IF (p))
    • Immunofluorescence (IF)
    • Flow Cytometry (FACS)
    • Immunofluorescence (Cultured Cells) (IF (cc))
    • Immunocytochemistry (ICC)
    • Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    • Immunoprecipitation (IP)
    • Immunohistochemistry (Frozen Sections) (IHC (fro))
    • Enzyme Immunoassay (EIA)
    • Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))
    • Immunofluorescence (fixed cells) (IF/ICC)
    • Dot Blot (DB)
    • Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
    • Immunochromatography (IC)
    • Functional Studies (Func)
    • Radioimmunoassay (RIA)
    • Neutralization (Neut)
    • Chromatin Immunoprecipitation (ChIP)
    • Coating (Coat)
    • Staining Methods (StM)
    • Immunostaining (ISt)
    • Fluorescence Microscopy (FM)
    • Immunoassay (IA)
    • Immunodiffusion (ID)
    • Proximity Ligation Assay (PLA)
    • Lateral Flow (LF)
    • Surface Plasmon Resonance (SPR)
    • Confocal Microscopy (CM)
    • Immunoelectrophoresis (IEP)
    • Live Cell Imaging (LCI)
    • Bio-Layer Interferometry (BLI)
    • Cytotoxicity Test (CyTox)
    • Indirect Immunofluorescence Assay (IFA)
    • RNA Interference (RNAi)
    • Antibody Array (AA)
    • Blocking Antibody (Inhibition)
    • Blocking Reagent (BR)
    • Blocking Peptide (BP)
    • ELISA (Capture)
    • Chemiluminescence Immunoassay (CLIA)
    • ChIP DNA-Sequencing (ChIP-seq)
    • In vivo Studies (in vivo)
    • Protein Assay (PrA)
    • Affinity Purification (AP)
    • Cellular Assay (CA)
    • Intracellular Flow Cytometry (ICFC)
    • Luminex Assay (LMNX)
    Please use search to find more...
  • Reactivity
    • Human
    • Mouse
    • Rat
    • Cow
    • Dog
    • Rabbit
    • Horse
    • Guinea Pig
    • Pig
    • Monkey
    • Zebrafish (Danio rerio)
    • Chicken
    • Bat
    • Sheep
    • Hamster
    • Xenopus laevis
    • Goat
    • Saccharomyces cerevisiae
    • Drosophila melanogaster
    • Arabidopsis thaliana
    • Virus
    • E. coli
    • Cat
    • Various Species
    • Plant
    • Human Immunodeficiency Virus (HIV)
    • Mammalian
    • Frog
    • Fish
    • Bacteria
    • Hepatitis C Virus (HCV)
    • Aequorea victoria
    • Oryza sativa
    • Chimpanzee
    • Non-Human Primate
    • Zea mays
    • Primate
    • C. elegans
    • Rhesus Monkey
    • SARS Coronavirus-2 (SARS-CoV-2)
    • Tag
    • Chemical
    • Chlamydomonas reinhardtii
    • Cynomolgus
    • Avian
    • Barley
    • Nicotiana tabacum
    • Arabidopsis
    • Influenza A Virus
    • Hepatitis B Virus (HBV)
    Please use search to find more...
  • Cited in publications
  • Images available
  • Independent Validation
  • Preservative free only
  • Carrier free only
  • Detection Method
    • Fluorometric
  • Host
    • Rabbit
    • Mouse
    • Goat
    • Sheep
    • Rat
    • Chicken
    • Guinea Pig
    • Human
    • Hamster
    • Alpaca
    • Mammalian Cells
    • Humanized
    • Chicken egg
    • Llama
    • Cell culture
    • Human Cells
    • Armenian Hamster
    • Cow
    • HEK-293F Cells
    • Chicken eggs
    • Golden Syrian Hamster
    • Horse
    • CHO Cells
    • Dog
    • Donkey
    • Carp
    • Fruit Fly (Drosophila melanogaster)
    • Phage Display
    • Pig
    • Tobacco (Nicotiana benthamiana)
  • Clonality
    • Polyclonal
    • Monoclonal
    • Chimeric
    • Bispecific
  • Clone
    • C1
    • C2
    • C3
    • C4
    • C5
    • C6
    • C10
    • C7
    • C11
    • D1
    • D4
    • C8
    • 1F6
    • 1D6
    • C12
    • D2
    • 2C4
    • 2B11
    • D3
    • 1F4
    • C9
    • 1C4
    • 1D2
    • 3G1
    • 1F10
    • 2D3
    • 1F3
    • 2E9
    • 2F11
    • 1A8
    • 1F11
    • 1A7
    • 1D4
    • 1E8
    • 1F8
    • 1G2
    • 1G5
    • 3F8
    • 3G4
    • 1A5
    • 1B2
    • 1D8
    • 1C1
    • 1E1
    • 2C1
    • 1A4
    • 2D7
    • 1A1
    • 1B4
    • 1B7
    Please use search to find more...
  • Binding Specificity
    • C-Term
    • N-Term
    • Center
    • Internal Region
    • Middle Region
    • AA 101-200
    • AA 1-100
    • AA 1-30
    • AA 51-150
    • AA 201-300
    • AA 151-250
    • AA 21-120
    • AA 301-400
    • AA 251-350
    • AA 1-50
    • AA 31-130
    • AA 401-500
    • AA 351-450
    • AA 121-220
    • AA 501-600
    • AA 131-230
    • AA 451-550
    • Extracellular
    • AA 1-80
    • AA 81-180
    • AA 61-160
    • AA 231-330
    • Extracellular Domain
    • AA 221-320
    • AA 41-140
    • AA 35-84
    • AA 71-120
    • AA 601-700
    • AA 161-260
    • Intracellular
    • AA 331-430
    • AA 51-100
    • AA 50-100
    • AA 181-280
    • AA 701-800
    • Cytosolic
    • AA 551-650
    • AA 1-300
    • AA 141-240
    • AA 1-110
    • AA 30-110
    • AA 1-200
    • AA 107-156
    • AA 251-300
    • AA 65-160
    Please use search to find more...
  • Conjugate
    • Un-conjugated
    • Biotin
    • FITC
    • HRP
    • PE
    • APC
    • Cy3
    • AbBy Fluor® 594
    • Cy5
    • AbBy Fluor® 350
    • AbBy Fluor® 488
    • AbBy Fluor® 555
    • AbBy Fluor® 647
    • Cy5.5
    • Cy7
    • AbBy Fluor® 750
    • AbBy Fluor® 680
    • Alexa Fluor 350
    • Alexa Fluor 405
    • Alexa Fluor 647
    • Alexa Fluor 750
    • Alexa Fluor 488
    • Alexa Fluor 594
    • Alexa Fluor 555
    • Alexa Fluor 680
    • CF®740
    • CF®488A
    • CF®405S
    • CF®568
    • CF®640R
    • CF®647
    • CF®594
    • PE-Cy5
    • PE-Cy7
    • PE-Cy5.5
    • PerCP
    • Atto 488
    • Atto 390
    • Atto 594
    • Alkaline Phosphatase (AP)
    • Peroxidase (POD)
    • DyLight 488
    • Texas Red (TR)
    • DyLight 680
    • DyLight 800
    • DyLight 405
    • DyLight 549
    • DyLight 649
    • CF®555
    • Rhodamine
    • His tag
  • Isotype
    • IgG
    • Ig Fraction
    • IgG1
    • IgG2a
    • IgG1 kappa
    • IgG2b
    • kappa
    • IgG2a kappa
    • IgG2b kappa
    • IgG2
    • IgM
    • IgY
    • IgG3
    • IgG kappa
    • IgM kappa
    • IgG3 kappa
    • IgG2c
    • IgG1 lambda
    • IgG Mix lambda
    • IgG2a lambda
    • IgG2c kappa
    • L234A
    • L235A
    • P329G
    • IgG2b lambda
    • IgG lambda
    • IgG4
    • IgG2 lambda
    • Ig kappa
    • IgG1/IgG2b
    • IgG2 kappa
    • lambda
    • IgA
    • IgA kappa
    • IgG Mix kappa
    • D265A
    • IgG1b kappa
    • IgG3 lambda
    • IgG IgA IgM Mix lambda
    • IgG1a
    • IgG4 kappa
    • IgM lambda
    • Ig
    • Ig Mix
    • IgG2c lambda
    • Fc Silenced
    • IgA2 kappa
    • IgE
    • IgE kappa
    • IgG IgA IgM Mix
    Please use search to find more...
  • Protein Type
    • Recombinant Antibody
  • Format
    • Liquid
    • Lyophilized
    • Frozen
    • Lyophilized or Liquid
  • Fragment
    • single-domain Antibody (sdAb)
    • Fc fragment
    • Fab fragment
    • scFv fragment
    • F(ab')2 fragment
  • Grade
    • Verified
    • KD Validated
    • KO Validated
    • Carrier-free
    • Cell Culture Grade
    • In vivo Grade
    • KO Validated, KD Validated
    • Research Grade
    • ChIP-seq Grade
    • Animal-Free
    • GMP Grade
    • ChIP Grade
    • Calibrator Grade
    • MALS verified
    • Recent
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    • Biomatik
    • Cloud-Clone
    • NSJ Bioreagents
    • FabGennix
    • RayBiotech
    • Rockland
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    • CUSABIO
    • Agrisera
    • Alomone Labs
    • Enogene Biotech
    • Antibodies Incorporated
    • Abiocode
    • OriGene
    • Wuhan Fine biotech
    • Elabscience
    • Synaptic Systems
    • Abeomics
    • RevMAb Biosciences
    • Active Motif
    • QED Bioscience
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    Please use search to find more...
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