Functional Bead Conjugation Buffer Set
IA, FACS
Catalog No. ABIN2690896
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Quick Overview for Functional Bead Conjugation Buffer Set (ABIN2690896)
Application
Immunoassay (IA), Flow Cytometry (FACS)
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Characteristics
- The Functional Bead Conjugation Buffer Set contains the supporting reagents necessary to covalently link water-soluble proteins to the surface of functional beads . The beads, once linked to a protein, can be used as a capture matrix for analytes of interest . The Functional Bead Conjugation Buffer Set contains enough material for 15 coupling reactions . Each coupling reaction yields approximately 3 - 6 ×10^6 beads which is enough material to run 500 or 1000 tests at a concentration of 6,000 beads per well . For a 500 test conjugation reaction 90 μg of protein is needed, and for a 1000 test conjugation reaction 180 μg of protein is needed . After covalently linking protein to the beads, it is recommended that a test be performed to confirm the success of the reaction . For that purpose, BD Biosciences offers three different phycoerythrin (PE) Functional Bead Ig Detectors that can be used to confirm the presence of antibody covalently linked to the beads.
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Components
- Coupling buffer and storage buffer
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Blocking Buffer for Fluorescent Western Blotting
CA, ELISA, IF, IHC, IP, MA, WB 1 X
10X TTBS Buffer pH 7.5ELISA, WB 10 X
Blotto Blocking Reagent Immunoanalytical Grade (Non-Fat Dry Milk)Analytical Grade BCA, ELISA, FM, IP, WB 1 X
10X PBS Buffer pH 7.2ELISA, FACS, IF, WB 10 X
0.5 M EDTA Buffer pH 8.0FACS 0.5 M
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Restrictions
- For Research Use only
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Format
- Liquid
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Storage
- 4 °C
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