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FACS and IF Staining Buffer with Goat Serum

FACS, FM, IHC 10 X
Catalog No. ABIN5706393
  • Application
    Flow Cytometry (FACS), Fluorescence Microscopy (FM), Immunohistochemistry (IHC)
    Purification
    FACS and IF Staining Buffer was aseptically filtered through a Millipore 0.22 micron filter into clean, pre-sterilized containers. The product was tested on trypticase soy agar for 24 hours, 48 hours and 72 hours and was found to be negative for bacteria.
    Sterility
    Aseptic filtered
  • Application Notes

    Flow Cytometry Dilution: User Optimized

    Immunohistochemistry Dilution: User Optimized

    Application Note: Fluorescent Activated Cell Sorting (FACS) and Immunofluorescence (IF) staining buffer is adequate for cell staining, washing and dilution in flow cytometry and immunofluorescence experiments.

    IF Microscopy Dilution: User Optimized

    Comment

    Synonyms: Fluorescent Activated Cell Sorting (FACS), Staining buffer, Flow cytometry staining buffer, Immunofluorescence staining buffer

    Background: Fluorescent Activated Cell Sorting (FACS) and Immunofluorescence (IF) staining buffer is a diluent buffer optimized for use in flow cytometry and immunofluorescence assay of viable and/or fixed cells. FACS and IF staining, washing buffer contains 2 % goat serum as carrier and stabilizer to reduce non-specific binding of antibodies and fluorochrome reagents to targets cells. Metabolic inhibitors and NaN3 are also included in the flow cytometry and immunofluorescence buffer to prevent patching, capping of surface antigen and bacterial growth.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    10 X
    Buffer

    Stabilizer: None

    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C
    Expiry Date
    3 months
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