CYP3A25 Protein (AA 1-503) (His tag)
custom-made
CYP3A25
Origin: Mouse
Host: HEK-293 Cells
Recombinant
> 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
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- Target
- CYP3A25 (Cytochrome P450, Family 3, Subfamily A, Polypeptide 25 (CYP3A25))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-503
- Origin
- Mouse
- Source
- HEK-293 Cells
- Purification tag / Conjugate
- This CYP3A25 protein is labelled with His tag.
- Purpose
- Predefined custom protein recombinant Cyp3a25 Protein expressed in mammalian cells.
- Sequence
- MELIPNLSIE TWVLLVTSLV LFYIYGTYSH GLFKKLGIPG PKPLPLLGTI FNYYDGMWKF DEDCYKKYGK IWGFYEGPQP ILAIMDPEII KIVLVKECYS VFTNRRFFGP VGFMKKAITI SEDEEWKRLR TLLSPTFTSG KLKEMFPIMR QYGDILVRNL RREEEKGEPI SMKDIFGAYS MDVITGTSFG VNVDSLNNPQ DPFVQKAKKI LKFKIFDPFL LSIILFPFLT PIYEMLNFSI FPRDSMNFFK KFVKRMKKER LASNQKNRVD FLQLMMNTQN SKGQESQKAL SDLEMAAQAV IFIFGGYDAT STSISLIMYE LATHPDVQKK LQDEIDRTLP NKAPVTYDAL MDMEYLDMVV NESLRLYPIA IRLERVSKKD VEINGVFIPK GTVVMIPIYP LHRNPEYWPE PQEFCPERFS KENKGNIDPY IYMPFGNGPR NCIGMRFALI SIKLAVIGVL QNFTVQPCEE TQIPLKISRE PIFQPEKPII LKVVSRDKPR TGS Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.
- Specificity
- If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.
- Characteristics
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Key Benefits:
- Predefined custom protein - from design to production - by highly experienced protein experts.
- Protein expressed in mammalian cells and purified in one-step affinity chromatography
- The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
If you are not interested in a full length protein, please contact us for individual protein fragments.
The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified. - Purity
- > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
- Grade
- custom-made
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- Application Notes
- We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- The buffer composition is at the discretion of the manufacturer.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C.
- Expiry Date
- 12 months
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- Target
- CYP3A25 (Cytochrome P450, Family 3, Subfamily A, Polypeptide 25 (CYP3A25))
- Alternative Name
- Cyp3a25
- Background
- Cytochrome P450 3A25 (EC 1.14.14.1) (CYPIIIA25),FUNCTION: Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.
- Molecular Weight
- 58.1 kDa
- UniProt
- O09158
- AlphaFold
- O09158
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