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Protease S. aureus

1 mg/mL
Catalog No. ABIN5706431
  • Purification
    Protease S. aureus V8 (Endoproteinase-Glu-C) specifically cleaves peptide bonds on the COOH-terminal side of either aspartic or glutamic acids. In the presence of ammonium, the enzyme specificity is limited to glutamic sites. It has a molecular weight of 27,000 daltons and optimum pH's of 4.0 and 7.8 with hemoglobin as the substrate. Protease S. aureus V8 is inhibited by diisopropylfluorophosphate and monovalent anions such as F-, Cl-, CH3COO- and NO3. Enzyme activity is determined by the casein digestion assay.
    Biological Activity Comment
    880 units/mg
  • Application Notes

    Application Note: Protease S. aureus has shown that one unit causes a change of 0.001 A280 nm per minute at 37° C at  pH 7.8 using casein as a substrate. Specific conditions for reactivity should be optimized by end user.

    Comment

    Synonyms: Glutamyl endopeptidase,Endoproteinase Glu-C, Staphylococcal serine proteinase, V8 protease, V8 proteinase

    Background: Protease S. aureus V8 (Endoproteinase-Glu-C) specifically cleaves peptide bonds on the COOH-terminal side of either aspartic or glutamic acids. In the presence of ammonium, the enzyme specificity is limited to glutamic sites. It has a molecular weight of 27,000 daltons and optimum  pH 's of 4.0 and 7.8 with hemoglobin as the substrate. Protease S. aureus V8 is inhibited by diisopropylfluorophosphate and monovalent anions such as F-, Cl-, CH3COO- and NO3. Enzyme activity is determined by the casein digestion assay. Protease S. aureus is ideal for investigators involved in enzyme and infectious diease research.

    Gene Name: sspA

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution

    Reconstitution Volume: 1.0 mL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    Concentration
    1 mg/mL
    Buffer

    Buffer: None

    Stabilizer: None

    Preservative
    Without preservative
    Storage
    4 °C,-20 °C
    Expiry Date
    12 months
  • Gene ID
    1280
    UniProt
    P0C1U8
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