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Proteinase K Solution

DNAIso, PD 20 mg/mL
Catalog No. ABIN7448222
  • Application
    DNA Isolation (DNAIso), Protein Digestion (PD)
    Specificity
    30-40 Units/mg dry weight
    Characteristics
    Proteinase K is one of the most active endopeptidases known. It rapidly inactivates endogenous nucleases including RNases and DNases making it suitable for the degradation of proteins during the isolation and manipulation of RNA and DNA. Proteinase K may also be used to permeabilize cells and tissues prior to labeling with in situ Apoptosis Detection Kits. Permeabilization of the sample by Proteinase K creates pores in the cell membrane and facilitates access to the DNA by biotinylated dNTPS.
    Purity
    > 95% (reducing SDS-PAGE)
    Biological Activity Comment
    Active between 37 - 70 °C
  • Application Notes
    Optimal working dilution should be determined by the investigator.
    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    20 mg/mL
    Storage
    -20 °C
    Storage Comment
    -25°C to -15°C
    Expiry Date
    36 months
  • Molecular Weight
    29.3 kDa
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