Hydroxy-delta-5-Steroid Dehydrogenase, 3 beta and Steroid delta-Isomerase 4 (HSD3B4) (AA 1-373) protein (His tag)
custom-made
HSD3B4
Origin: Mouse
Host: HEK-293 Cells
Recombinant
> 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
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- Target
- Hydroxy-delta-5-Steroid Dehydrogenase, 3 beta and Steroid delta-Isomerase 4 (HSD3B4)
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-373
- Origin
- Mouse
- Source
- HEK-293 Cells
- Purification tag / Conjugate
- His tag
- Purpose
- Predefined custom protein recombinant Hsd3b4 Protein expressed in mammalian cells.
- Sequence
- MPGWSCLVTG AGGFLGQRIV RMLVQEEELQ EIRALFRTFG RKQEEELSKL QTKTKVTVLK GDILDAQCLK RACQGMSAVI HTAAAIDPLG AASRQTILDV NLKGTQLLLD ACVEANVPTF IYSSSVLVAG PNSYKEIILN AHEEEHHEST WSNPYPYSKK MAEKAVLAAN GSILKNGGTL HTCALRLSFI YGEECQVTST TVKTALKNNS IIKKNATFSI ANPVYVGNAA WAHILAARSL QDPKKSPSIQ GQFYYITDDT PHQSYDDLKC TLSKEWGLRL DTSWSLPLPL LYWLAFLLET VSFLLRPVYN YRPPFNRLLI TVLNSVFTFS YKKAQRDLGY EPLVSWEEAK QKTSEWIGTL VMQHREIGNK KSQ Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.
- Specificity
- If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.
- Characteristics
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Key Benefits:
- Predefined custom protein - from design to production - by highly experienced protein experts.
- Protein expressed in mammalian cells and purified in one-step affinity chromatography
- The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
If you are not interested in a full length protein, please contact us for individual protein fragments.
The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified. - Purity
- > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
- Grade
- custom-made
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- Application Notes
- We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- The buffer composition is at the discretion of the manufacturer.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C.
- Expiry Date
- 12 months
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- Target
- Hydroxy-delta-5-Steroid Dehydrogenase, 3 beta and Steroid delta-Isomerase 4 (HSD3B4)
- Alternative Name
- Hsd3b4
- Background
- NADPH-dependent 3-keto-steroid reductase Hsd3b4 (3 beta-hydroxysteroid dehydrogenase type 4) (3 beta-hydroxysteroid dehydrogenase type IV) (3 beta-HSD IV) (EC 1.1.1.270) (Dihydrotestosterone 3-ketoreductase) (EC 1.1.1.210),FUNCTION: Responsible for the reduction of the oxo group on the C-3 of 5alpha-androstane steroids. Catalyzes the conversion of dihydrotestosterone to its inactive form 5alpha-androstanediol, that does not bind androgen receptor/AR. Does not function as an isomerase. {ECO:0000269|PubMed:8145763}.
- Molecular Weight
- 41.8 kDa
- UniProt
- Q61767
- AlphaFold
- Q61767
- Pathways
- Metabolism of Steroid Hormones and Vitamin D, Steroid Hormone Biosynthesis
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