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Protease Inhibitor Cocktail

BR, InhA

Catalog No. ABIN2866133

$241.54

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Quick Overview for Protease Inhibitor Cocktail (ABIN2866133)

Application

Blocking Reagent (BR), Inhibition Assay (InhA)

Specificity

Protease Inhibitor Cocktail is a broad-spectrum cocktail designed to prevent proteolytic degradation during lysis and extraction procedures from mammalian cells or tissues.

Characteristics

The cocktail contains a broad-spectrum of inhibitors targeting cysteine proteases, serine proteases, aspartic acid proteases, and aminopeptidases. In addition to being ready-to-use, the Protease Inhibitor cocktail is also EDTA-free. The cocktail is supplied as a 100X stock solution in a liquid format that has been stabilized in dimethylsulfoxide (DMSO) for improved accuracy, solubility, and ease of use in comparison to traditional tablets.
- AEBSF, 104 mM - Serine Proteases
- Aprotinin, 80 µM - Serine Proteases
- Bestatin, 4 mM - Aminopeptidases
- E-64, 1.4 mM - Cyseine Proteases
- Leupeptine, 2 mM - Serine and Cysteine Proteases
- Pepstatin A, 1.5 mM - Asparic Acid Proteases
Geldanamycin- FITC
Inh 30562-34-6 (4E,6Z,8S,9S,10E,12S,13R,14S,16R)-13-hydroxy-8,14,19-trimethoxy-4,10,12,16-tetramethyl-3,20,22-trioxo-2-azabicyclo[16.3.1] docosa-1(21),4,6,10,18-pentaen-9-yl carbamate

Geldanamycin
Inh 30562-34-6 (4E,6Z,8S,9S,10E,12S,13R,14S,16R)-13-hydroxy-8,14,19-trimethoxy-4,10,12,16-tetramethyl-3,20,22-trioxo-2-azabicyclo[16.3.1] docosa-1(21),4,6,10,18-pentaen-9-yl carbamate

Radicicol
Inh 12772-57-5

Geldanamycin- Biotin
Inh 30562-34-6 (4E,6Z,8S,9S,10E,12S,13R,14S,16R)-13-hydroxy-8,14,19-trimethoxy-4,10,12,16-tetramethyl-3,20,22-trioxo-2-azabicyclo[16.3.1] docosa-1(21),4,6,10,18-pentaen-9-yl carbamate

Herbimycin A
Inh 70563-58-5 [(2R,3S,5S,6R,7S,8E,10S,11S,12E,14E)-2,5,6,11-tetramethoxy-3,7,9,15-tetramethyl-16,20,22-trioxo-17-azabicyclo[16.3.1]docosa-1(21),8,12,14,18-pentaen-10-yl] carbamate

17-GMB-APA-GA
Inh [(3R,5S,6R,7S,10S,11S)-21-[3-[4-(2,5-dioxopyrrol-1-yl)butanoylamino]propylamino]-6-hydroxy-5,11-dimethoxy-3,7,9,15-tetramethyl-16,20,22-trioxo-17-azabicyclo[16.3.1]docosa-1(21),8,12,14,18-pentaen-10-yl] carbamate

TEMPO
Inh 2564-83-2 2,2,6,6-Tetramethylpiperidine 1-oxyl, 2,2,6,6-Tetramethyl-1-piperidinyloxy, free radical, (2,2,6,6-Tetramethyl-1-piperidinyl)oxidanyl, 1,1,5,5-tetramethylpentamethylene nitroxide, 2,2,6,6-Tetramethylpiperidine-1-oxyl

Triacsin C
Inh 76896-80-5 (3E)-1-Oxo-3-[(2E,4E,7E)-2,4,7-undecatrien-1-ylidene]triazane

TPCK
Inh 402-71-1 N-(4-Chloro-3-oxo-1-phenyl-2-butanyl)-4-methylbenzenesulfonamide

Staurosporine
Inh 62996-74-1 (2S,3R,4R,6R)-3-Methoxy-2-methyl-4-(methylamino)-29-oxa-1,7,17-triazaoctacyclo[12.12.2.12,6.07,28.08,13.015,19.020,27.021,26]nonacosa-8,10,12,14,19,21,23,25,27-nonaen-16-one

Comment
The cocktail solution inhibits aminopeptidases and cysteine, serine and aspartic acid proteases. Protease Inhbitor Cocktail is provided EDTA-free, as a ready-to-use 100X stock solution in DMSO. The cocktail composition and inhibitor targets are listed below:
- AEBSF, 104 mM - serine proteases
- Aprotinin, 80 μM - serine protease
- Bestatin, 4 mM - aminopeptidases
- E-64, 1.4 mM - cysteine proteases
- Leupeptin, 2 mM - serine and cysteine proteases
- Pepstatin A, 1.5 mM - aspartic acid proteases
It is recommended to use 10 μl of the cocktail solution for every 1 ml of cell lysate. Since protease levels may vary among cell and tissue types, it may be necessary to increase the concentration of inhibitors.
Restrictions

For Research Use only
Format

Liquid

Storage

-20 °C

Storage Comment

The Protease Inhibitor Cocktail is provided in DMSO as a faint yellow solution. The product is shipped at room temperature and should be stored at -20 °C long-term.