Guinea Pig IgG isotype control (FITC)
Quick Overview for Guinea Pig IgG isotype control (FITC) (ABIN1043589)
Target
Fragment
Conjugate
Application
Isotype
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Host
- Guinea Pig
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Supplier Product No.
- 006-0204
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Supplier
- Rockland
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Purpose
- Guinea Pig IgG F(ab')2 Fragment Fluorescein Conjugated
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Cross-Reactivity (Details)
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Guinea Pig IgG, anti-Guinea Pig IgG F(ab')2 and anti-Guinea Pig Serum. No reaction was observed against anti-Guinea Pig IgG F(c) or anti-Pepsin.
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Characteristics
- Guinea Pig IgG F(ab')2 fragment fluorescein conjugated, Guinea Pig IgG F(ab')2 fragment FITC conjugated
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Purification
- This product was prepared from normal serum by delipidation, salt fractionation and ion change chromatography followed by pepsin digestion and extensive dialysis against the buffer stated above.
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Labeling Ratio
- 2.9
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Hamster IgG isotype control
ELISA, WB, IHC, SDS IgG unconjugated
Rabbit IgG isotype controlELISA, WB, IHC, SDS IgG unconjugated
Rabbit IgG isotype controlFACS, IHC, IP Polyclonal IgG unconjugated
Rabbit IgG isotype control (Atto 550)FACS, IHC Polyclonal IgG Atto 550
Goat IgG isotype controlIsoC, ELISA, IHC Polyclonal IgG unconjugated
Rabbit IgG isotype controlIsoC, FACS, ELISA, IHC Polyclonal IgG unconjugated
Rabbit IgG isotype controlFACS, ELISA Polyclonal IgG unconjugated
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Application Notes
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Application Note: Guinea Pig IgG F(ab')2 fragment Fluorescein conjugated has been tested in dot blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA), fluorescent western blotting, multiplex analysis, including multicolor imaging, and utilizing various commercial platforms.
Other: User Optimized
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
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Reconstitution Buffer: Restore with deionized water (or equivalent)
Reconstitution Volume: 1.0 mL
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Concentration
- 1.0 mg/mL
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Buffer
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Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Thimerosal -
Preservative
- Thimerosal (Merthiolate)
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Precaution of Use
- This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Storage
- 4 °C,-20 °C
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Storage Comment
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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Expiry Date
- 12 months
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- IgG
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Target Type
- Antibody
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Background
- Background: Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. This product possesses the F(ab')2 fragment, recognized by the two F(ab) fragments yielded from the digestion of the antibody below the disulfide bond hinge region.
Target
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