Human IgG Isotype Control

Details for Product No. ABIN1043618, Supplier: Log in to see
Isotype
Host
Human
178
174
172
139
127
116
83
67
64
63
57
52
51
47
33
22
16
15
11
7
7
4
3
Application
Isotype Control (IsoC), ELISA, Western Blotting (WB)
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Isotype IgG
Fragment Fab fragment
Characteristics Concentration Definition: by UV absorbance at 280 nm
Isotype
Background Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. The F(ab) fragment is the portion of the antibody that binds to the antigen target. The immunoglobulin Fab also possesses one constant and one variable region of both the heavy and light chain.

Synonyms: Human Immunoglobulin Fab, F(ab), Fragment antigen-binding
Application Notes Human IgG Fab Fragment can be utilized as a control or standard reagent in Western Blotting and ELISA experiments.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Restore with deionized water (or equivalent)
Concentration 2.0 mg/mL
Buffer 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Storage 4 °C
Supplier Images
 image for Human IgG isotype control (ABIN1043618) Human IgG isotype control
SDS-PAGE (SDS) image for Human IgG isotype control (ABIN1043618) SDS-Page of Human Fab. Lane 1: Human Fab - Non-Reduced. Lane 2: Human Fab - Reduced. ...
SDS-PAGE (SDS) image for Human IgG isotype control (ABIN1043618) SDS-PAGE results of Human IgG Fab Fragment. Lane 1: reduced Human IgG Fab Fragment (5...