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Mouse IgG Isotype Control

FACS, ELISA Polyclonal IgG unconjugated
Catalog No. ABIN376362

Quick Overview for Mouse IgG Isotype Control (ABIN376362)

Target

IgG

Clonality

  • 289
  • 71
Polyclonal

Application

Flow Cytometry (FACS), ELISA

Isotype

IgG
  • Host

    • 135
    • 125
    • 123
    • 84
    • 82
    • 58
    • 55
    • 52
    • 46
    • 42
    • 38
    • 33
    • 33
    • 32
    • 25
    • 18
    • 10
    • 8
    • 8
    • 7
    • 3
    • 2
    Mouse

    Characteristics

    Mouse IgG-LE/AF

    Purification

    Purified

    Endotoxin Level

    Low endotoxin
  • Application Notes

    • Applications: Quality tested applications include - FC , ELISA , FLISA
    • Other referenced applications include - IHC-PS , ICC , WB , IP , ChIP , Block , In vitro control , In vivo control
    • Working Dilutions: Flow Cytometry FITC and BIOT conjugates 1 g/106 cells PE, PE/TXRD, APC, SPRD, PE/CY5.5, PE/CY7, APC/CY5.5, and 0.1 g/106 cells APC/CY7 conjugates For flow cytometry, the suggested use of these reagents is in a final volume of 100 L ELISA AP conjugate 1:500 - 1:5,000

    Sample Volume

    1 mL

    Restrictions

    For Research Use only
  • Concentration

    0.5 mg/mL

    Buffer

    0.5 mg purified immunoglobulin in 1.0 mL of PBS

    Preservative

    Azide free

    Handling Advice

    Each reagent is stable for the period shown on the bottle label if stored as directed.

    Storage

    -20 °C

    Storage Comment

    Aliquot and freeze the low endotoxin, azide-free product at -20 °C immediately upon receipt.
  • Guerriero, Palmieri, De Marco, Cossu, Remondelli, Capunzo, Turco, Rosati: "The anti-apoptotic BAG3 protein is involved in BRAF inhibitor resistance in melanoma cells." in: Oncotarget, Vol. 8, Issue 46, pp. 80393-80404, (2017) (PubMed).

    Iorio, Festa, Rosati, Hahne, Tiberti, Capunzo, De Laurenzi, Turco: "BAG3 regulates formation of the SNARE complex and insulin secretion." in: Cell death & disease, Vol. 6, pp. e1684, (2015) (PubMed).

    Aeckerle, Drummer, Debowski, Viebahn, Behr: "Primordial germ cell development in the marmoset monkey as revealed by pluripotency factor expression: suggestion of a novel model of embryonic germ cell translocation." in: Molecular human reproduction, Vol. 21, Issue 1, pp. 66-80, (2015) (PubMed).

  • Target

    IgG

    Target Type

    Antibody
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