Mouse IgG2a Isotype Control
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- Target See all IgG2a products
- IgG2a
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Host
- Mouse
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Clonality
- Monoclonal
- Application
- Flow Cytometry (FACS), ELISA, Western Blotting (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Functional Studies (Func), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Specificity
- This mouse IgG2a monoclonal antibody (clone PPV-04) reacts with undefined epitope on a plant pathogen.
- No Cross-Reactivity
- Human, Mouse, Pig, Rat
- Purification
- Purified by protein-A affinity chromatography.
- Purity
- > 95 % (by SDS-PAGE)
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Clone
- PPV-04
- Isotype
- IgG2a
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- Application Notes
- Negative control: The reagent is intended as an isotype control to establish the amount of non-specific antibody binding. For your particular experiment, use the same concentration of this control antibody as the recommended working concentration of the antigen-specific antibody. Also, when working with prediluted antibodies, dilute the isotype control to the same concentration as is the concentration of the antigen-specific antibody in the prediluted antibody solution you are using. If under particular experimental conditions the background signal of the isotype control is too high (usually when working concentrations of used antibodies are above 10 μg/mL of incubation mixture), change the conditions of your experiment to reduce the background.
- Restrictions
- For Research Use only
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- Concentration
- 1 mg/mL
- Buffer
- Phosphate buffered saline (PBS), pH 7.4
- Preservative
- Azide free
- Handling Advice
- Do not freeze.
- Storage
- 4 °C
- Storage Comment
- Store at 2-8°C. Do not freeze.
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- Target
- IgG2a
- Abstract
- IgG2a Products
- Synonyms
- gamma-2a immunoglobulin heavy chain Isotype Control, IgG-2a Isotype Control
- Target Type
- Antibody
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