Rat IgG2a isotype control (PE)
Quick Overview for Rat IgG2a isotype control (PE) (ABIN5608810)
Target
Clonality
Conjugate
Application
Clone
Isotype
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Host
- Rat
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Supplier Product No.
- 012-008-041
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Supplier
- Rockland
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Purpose
- Rat IgG2a Isotype Control Phycoerythrin Conjugated
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Cross-Reactivity (Details)
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rat IgG and anti-Rat serum. Isotyping assay resulted non-reactive with antisera to Rat IgG1, IgG2b, IgG3, IgM, and IgA. Phycoerythrin Conjugated Rat IgG2a confirmed by Dot Blot.
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Characteristics
- Rat Isotype Control, Rat IgG2a PE isotype, RAT IgG2a subclass isotype control
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Purification
- RAT IgG2a isotype control has been prepared from concentrated cell culture supernatant by immunoaffinity chromatography using protein G.
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Labeling Ratio
- 1-2
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Mouse IgG2a isotype control (APC)
FACS, IsoC Monoclonal MOPC-173 IgG2a APC
Mouse IgG2a isotype control (PerCP)FACS, IsoC Monoclonal MOPC-173 IgG2a PerCP
Rat IgG2a isotype control (FITC)FACS, IsoC Monoclonal RTG2A1-1 IgG2a FITC
Mouse IgG2a isotype control (FITC)FACS, IsoC Monoclonal MOPC-173 IgG2a FITC
Mouse IgG2a isotype control (PE)FACS, IsoC Monoclonal MOPC-173 IgG2a PE
Mouse IgG2a isotype control (Biotin)FACS, IsoC, ELISA, WB, IP, ICC, IHC (fro), IHC (p) Monoclonal MOPC-173 IgG2a Biotin
Mouse IgG2a isotype controlFACS, IsoC, ELISA, WB, IP, ICC, IHC (fro), Func, IHC (p) Monoclonal MOPC-173 IgG2a unconjugated
Mouse IgG2a isotype controlFACS, IsoC, ELISA, WB, IP, ICC, IHC (fro), IHC (p) Monoclonal MOPC-173 IgG2a unconjugated
Mouse IgG2a isotype controlFACS, IsoC, ELISA, IHC Monoclonal HOPC-1 IgG2a unconjugated
Mouse IgG2a isotype control (Cy5)FACS, ELISA Monoclonal HOPC-1 IgG2a Cy5
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Application Notes
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Flow Cytometry Dilution: 1:1000-1:5000
Application Note: RAT IgG2a isotype control can be utilized as a control or standard reagent in Western Blotting, Flow Cytometry, and ELISA experiments where determination of sample isotype is important. Specific conditions should be optimized by user.
FLISA Dilution: User Optimized
ELISA Dilution: 1:2000 - 1:20,000
IF Microscopy Dilution: User Optimized
Other: User Optimized
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
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Reconstitution Buffer: Restore with deionized water (or equivalent)
Reconstitution Volume: 1.0 mL
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Concentration
- 0.5 mg/mL
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Buffer
- Optional[Buffer]: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free Preservative: 0.01 % (w/v) Sodium Azide
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Preservative
- Sodium azide
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Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Storage
- 4 °C
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Storage Comment
- Store vial at 4° C prior to restoration. Restore with deionized water (or equivalent). This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Centrifuge product if not completely clear after standing at room temperature. Do not freeze after reconstitution. Store reagent in the dark. Use subdued lighting during handling and incubation of cells prior to analysis.
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Expiry Date
- 12 months
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- IgG2a
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Target Type
- Antibody
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Background
- Background: RAT IgG2a isotype control is used in flow cytometry, western blot and ELISA and differentiate between immunoglobulin classes and subclasses. Isotype controls allow for the genetic variations or differences in the constant regions of the heavy and light chains. In Rat there are six relevant heavy chain isotypes and two light chain isotypes: heavy chain alpha - IgA, gamma - IgG 1, 2a, 2b, 2c and μ - IgM, light chain kappa and lambda.
Target
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