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Human IgG isotype control (PE)

FACS, WB, IHC IgG PE

Catalog No. ABIN5633236

Supplier Product No.: 009-008-002

$142.00

Plus shipping costs $50.00

0.5 mL

Shipping to: United States

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Quick Overview for Human IgG isotype control (PE) (ABIN5633236)

Target

IgG

Conjugate

PE

Application

Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (IHC)

Isotype

IgG

Host
137

136

135

94

92

60

53

51

44

40

37

33

32

31

23

18

14

14

11

8

3

2
Human

Supplier Product No.

009-008-002

Supplier

Rockland

Cross-Reactivity (Details)

Human IgG whole molecule Phycoerythrin conjugated was assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Phycoerythrin, anti-Human IgG and anti-Human Serum.

Purification

Human IgG whole molecule Phycoerythrin conjugated was prepared from normal serum delipidation, salt fractionation, ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Labeling Ratio

1-2
Rabbit IgG isotype control
IsoC Polyclonal IgG unconjugated

Hamster IgG isotype control
ELISA, WB, IHC, SDS IgG unconjugated

Rabbit IgG isotype control
FACS, ELISA, WB, IF (p), IF (cc), IHC (p), IHC (fro) Polyclonal IgG unconjugated

Rabbit IgG isotype control
ELISA, WB, IHC, SDS IgG unconjugated

Rabbit IgG isotype control
FACS, IHC, IP Polyclonal IgG unconjugated

Rabbit IgG isotype control (Atto 550)
FACS, IHC Polyclonal IgG Atto 550

Mouse IgG isotype control
FACS, ELISA, WB, IF (p), IF (cc), IHC (p), IHC (fro) Polyclonal IgG unconjugated

Goat IgG isotype control
IsoC, ELISA, IHC Polyclonal IgG unconjugated

Rabbit IgG isotype control
IsoC, FACS, ELISA, IHC Polyclonal IgG unconjugated

Rabbit IgG isotype control
FACS, ELISA Polyclonal IgG unconjugated

Application Notes

Flow Cytometry Dilution: 1:500-1:2500
Immunohistochemistry Dilution: 1:500-1:2500
Application Note: Human IgG whole molecule Phycoerythrin conjugated can be utilized as a control reagent in Flow Cytometry, Immunohistochemistry, and Western Blotting. Researchers should determine optimal titers for applications that are not stated.
Western Blot Dilution: 1:1,000-1:5,000

Restrictions

For Research Use only
Format

Lyophilized

Reconstitution

Reconstitution Volume: 1.0 mL
Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration

1.0 mg/mL

Buffer

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative

Sodium azide

Precaution of Use

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

4 °C

Storage Comment

Store vial at 4° C prior to opening. Dilute only prior to immediate use. Do not freeze after reconstitution. Store reagent in the dark. This product is stable at 4° C as an undiluted liquid. Use subdued lighting during handling and incubation of cells prior to analysis.

Expiry Date

12 months
Target

IgG

Target Type

Antibody

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