Rabbit IgG Isotype Control

Details for Product No. ABIN5633247
Isotype
Host
Rabbit
150
136
128
103
98
70
60
50
48
45
42
41
34
34
31
26
12
11
9
9
7
4
3
Application
ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
Options
Isotype IgG
Fragment Fab fragment
Cross-Reactivity (Details) Rabbit IgG Fab fragment was assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, anti-Rabbit IgG and anti-Rabbit IgG F(ab')2. No reaction was observed against anti-Rabbit IgG F(c) or anti-Papain.
Purification Rabbit IgG Fab fragment was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation, ion exchange chromatography and papain digestion followed by chromatographic separation and extensive dialysis against the buffer stated above.
Sterility Sterile filtered
Isotype
Application Notes

Immunohistochemistry Dilution: User Optimized

Application Note: Rabbit IgG Fab Fragment can be utilized as a control or standard reagent in Western Blotting and ELISA experiments.

Western Blot Dilution: User Optimized

ELISA Dilution: User Optimized
Restrictions For Research Use only
Format Liquid
Concentration 2.0 mg/mL
Buffer

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment Store vial at 4° C prior to opening. This product is stable 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
Expiry Date 12 months
Supplier Images
Image no. 1 for Rabbit IgG isotype control (ABIN5633247) SDS-PAGE of Rabbit IgG F(ab) Fragment . Lane M: 3 µL Opal Prestained Marker . Lane 1:...