Sheep IgG Isotype Control

Details for Product No. ABIN5633249
Isotype
Host
Sheep
150
136
129
103
98
70
59
50
48
45
42
41
34
34
31
26
12
11
9
9
7
4
3
Application
Isotype Control (IsoC), ELISA, Western Blotting (WB)
Options
Isotype IgG
Characteristics Concentration Definition: by UV absorbance at 280 nm
Isotype
Background Secreted as part of the adaptive immune response by plasma B cells, Sheep immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-afinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both heavy and light chains of the antibody molecule are present.

Synonyms: Sheep Immunoglobulin Gamma, Immunoglobulin G
Application Notes Sheep IgG whole molecule can be utilized as a control or standard reagent in Western Blotting and ELISA experiments.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Restore with deionized water (or equivalent)
Concentration 10.0 mg/mL
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Storage 4 °C
Supplier Images
Image no. 1 for Sheep IgG isotype control (ABIN5633249) SDS-Page of Sheep IgG Lane 1: Sheep IgG Non-Reduced Lane 2: Sheep IgG Reduced Load: 1...