Human IgG isotype control (Biotin)
-
- Target See all IgG products
- IgG
- Antibody Type
- Native
- Fragment
- Fc fragment
-
Host
- Human
- Conjugate
- Biotin
- Application
- Isotype Control (IsoC), ELISA, Western Blotting (WB)
- Characteristics
- Concentration Definition: by UV absorbance at 280 nm
- Isotype
- IgG
-
Rabbit IgG isotype control
IsoC, ELISA, WB IgG Native unconjugated
Mouse IgG isotype controlELISA, FACS Polyclonal IgG unconjugated
Goat IgG isotype controlELISA, WB, IHC IgG unconjugated
Rabbit IgG isotype control (PE)ELISA, FACS Polyclonal IgG PE
Goat IgG isotype controlELISA, FACS Polyclonal IgG unconjugated
Rabbit IgG isotype controlELISA, FACS Polyclonal IgG unconjugated
Goat IgG isotype controlIsoC, ELISA, IHC Polyclonal IgG unconjugated
Rat IgG isotype controlELISA, WB IgG unconjugated
-
- Application Notes
- Human IgG F(c) fragment Biotin conjugated can be utilized as a control reagent in both Western Blotting and ELISA format experiments.
- Restrictions
- For Research Use only
-
- Format
- Lyophilized
- Reconstitution
- Restore with deionized water (or equivalent)
- Concentration
- 1.0 mg/mL
- Buffer
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
- Preservative
- Sodium azide
- Precaution of Use
- WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
- Handling Advice
- Aliquot to Avoid repeated freezing and thawing.
- Storage
- 4 °C
-
- Target
- IgG
- Abstract
- IgG Products
- Target Type
- Antibody
- Background
-
Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. The F(c) fragment binds with very high affinity to the Fc receptor proteins on phagocytic leukocytes. When digested from the whole antibody molecule, the F(c) fragment no longer posses the epitope recognition site. This Human IgG whole molecule is conjugated to biotin (Vitamin H), a small biomolecule that has a large affinity for avidin and streptavidin.
Synonyms: Human Immunoglobulin G F(c) fragment, IgG Fc, Vitamin H, Coenzyme R
-
